Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A7 F1 I1 R1 | 776 | 60.0 | 3074904 | 90.1% | 2770488 | 104.4 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,238,562 | (T)7→6 | intergenic (+265/‑18) | ygjR → / → alx | putative NAD(P)‑dependent dehydrogenase/putative membrane‑bound redox modulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,238,556 | 0 | T | . | 100.0% | 172.5 / NA | 42 | intergenic (+259/‑24) | ygjR/alx | putative NAD(P)‑dependent dehydrogenase/putative membrane‑bound redox modulator |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (17/25); total (17/25) | |||||||||||
TGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGCCATGA > NC_000913/3238418‑3238688 | tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt > 1:805187/1‑139 (MQ=255)tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt > 1:1487474/1‑139 (MQ=255)tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTCTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt > 1:921910/1‑139 (MQ=255) aaaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttttt < 2:1254287/139‑1 (MQ=255) ccGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAgg < 2:1299032/139‑1 (MQ=255) ccGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAgg < 2:805187/139‑1 (MQ=255) ggTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGaa < 2:316349/139‑1 (MQ=255) ggTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGaa < 1:417426/139‑1 (MQ=255) ggTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGaa < 2:1487474/139‑1 (MQ=255) ggTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTCTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGaa < 2:921910/139‑1 (MQ=255) tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACt > 1:1183624/1‑139 (MQ=255) cGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGTGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTg > 2:1311549/1‑139 (MQ=255) gCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCGGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGt > 2:1277343/1‑139 (MQ=255) tgttgtAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACt < 2:195781/96‑1 (MQ=255) tgttgtAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACt > 1:195781/1‑96 (MQ=255) gaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt > 2:345500/1‑70 (MQ=255) gaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtttttt < 1:345500/70‑1 (MQ=255) cTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCa > 2:298584/1‑139 (MQ=255) cTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCa > 1:9013/1‑139 (MQ=255) tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGTCGtttttt < 1:200342/65‑1 (MQ=255) tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGTCGtttttt > 2:200342/1‑65 (MQ=255) tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAAt > 1:327715/1‑88 (MQ=255) tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAAt < 2:327715/88‑1 (MQ=255) ggAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGTCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGc < 1:270397/139‑1 (MQ=255) gAAGGCGAGGTCTATGCATAAAAAGCAGTGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCt < 1:1311549/139‑1 (MQ=255) cGAGGTCTATGCATAAAAAGCAGC‑GCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGc > 1:1063288/1‑90 (MQ=255) cGAGGTCTATGCATAAAAAGCAGC‑GCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGc < 2:1063288/90‑1 (MQ=255) aGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGAGGGAGGCCG‑TTGATTGTGTGTAAGGAACTTCTATGAATACTGTCGGCACGGAGTTGCTATGGGGCGGAGTCGCTGTTGTTGTCGCCATTATGCTGGCTATc > 2:270397/1‑139 (MQ=255) aGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGaa > 2:1008690/1‑65 (MQ=255) aGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGaa < 1:1008690/65‑1 (MQ=255) tGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCtgt < 2:1183624/139‑1 (MQ=255) aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAgg > 2:985726/1‑50 (MQ=39) aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAgg > 1:1308253/1‑50 (MQ=39) aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAgg < 2:1308253/50‑1 (MQ=39) aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAgg < 1:985726/50‑1 (MQ=39) aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGt < 2:932452/68‑1 (MQ=255) aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGt > 1:932452/1‑68 (MQ=255) aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg > 2:1398839/1‑78 (MQ=255) aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg < 1:1398839/78‑1 (MQ=255) aaaGCAGCGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCa > 1:44891/1‑139 (MQ=255) cagcGGCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAgggg < 1:298584/139‑1 (MQ=255) gCTGACGTCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGcgtcg < 1:1372855/139‑1 (MQ=255) tCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGca < 1:893043/139‑1 (MQ=255) tCTTCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGca < 1:1277343/139‑1 (MQ=255) ttCCGACGTTGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGcaca < 2:1408772/139‑1 (MQ=255) ttGGCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccatga < 2:1330470/139‑1 (MQ=255) ggCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCt < 1:1404119/102‑1 (MQ=255) ggCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCt > 2:1404119/1‑102 (MQ=255) ggCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGca < 2:1312610/127‑1 (MQ=255) ggCCG‑TTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGca > 1:1312610/1‑127 (MQ=255) | TGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGCCATGA > NC_000913/3238418‑3238688 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |