Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,277,867	C→T	V267I (GTC→ATC)	agaR ←	transcriptional repressor of the aga regulon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,277,867	0	C	T	100.0%	252.5 / NA	79	V267I (GTC→ATC)	agaR	transcriptional repressor of the aga regulon
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (38/41); total (38/41)

GTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAA  >  NC_000913/3277735‑3278000

gTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAAt                                                                                                                                 <  2:1129148/139‑1 (MQ=255)

     aaaGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACtt                                                                                                                            <  1:827597/139‑1 (MQ=255)

         agGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAAc                                                                                                                        >  1:98542/1‑139 (MQ=255)

           gACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGCAACCCATGGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAAccc                                                                                                                      <  2:1147329/139‑1 (MQ=255)

           gACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAAccc                                                                                                                      <  1:885018/139‑1 (MQ=255)

               tcctccTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGc                                                                                                                  <  2:424730/139‑1 (MQ=255)

                           cTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTcc                                                                                                      >  2:1137257/1‑139 (MQ=255)

                            tGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCt                                                                                                     >  1:793123/1‑139 (MQ=255)

                                 aaCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGAAGTCCTTCCAg                                                                                                >  1:995157/1‑139 (MQ=255)

                                  aCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAgg                                                                                               <  1:1356350/139‑1 (MQ=255)

                                     cTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTa                                                                                            >  2:685600/1‑139 (MQ=255)

                                        aCCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATcc                                                                                         >  2:373446/1‑139 (MQ=255)

                                        aCCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCAATTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATcc                                                                                         >  2:979503/1‑139 (MQ=255)

                                             agaAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAgg                                                                                    >  2:679256/1‑139 (MQ=255)

                                              gaAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGa                                                                                   <  1:679256/139‑1 (MQ=255)

                                                   agaagaAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGcc                                                                              <  2:793123/139‑1 (MQ=255)

                                                      agaaACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtc                                                                           >  2:1246629/1‑139 (MQ=255)

                                                        aaaCCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                         <  1:979503/139‑1 (MQ=255)

                                                         aaCCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcatc                                                                        >  1:1017444/1‑139 (MQ=255)

                                                         aaCCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcatc                                                                        <  2:182122/139‑1 (MQ=255)

                                                             cATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATcaaca                                                                    >  2:1420153/1‑139 (MQ=255)

                                                               ttGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGCTCAGAATCACTTCAACCCCAGCCTTACGCAGTCCTTCCAGGCTATCAGAAGGAATGCCTTCATCAACAAt                                                                  >  2:991149/1‑139 (MQ=255)

                                                               ttGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAAt                                                                  >  2:413340/1‑139 (MQ=255)

                                                                    ggTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACt                                                                                                                             >  1:757230/1‑75 (MQ=255)

                                                                    ggTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACt                                                                                                                             <  2:757230/75‑1 (MQ=255)

                                                                        ttCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAgg                                                                                               <  1:1182507/101‑1 (MQ=255)

                                                                        ttCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAgg                                                                                               >  2:1182507/1‑101 (MQ=255)

                                                                         tCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATcaaca                                                                    >  2:474729/1‑127 (MQ=255)

                                                                         tCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATcaaca                                                                    <  1:474729/127‑1 (MQ=255)

                                                                          cacaATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACtt                                                                                                                            >  2:555845/1‑70 (MQ=255)

                                                                          cacaATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACtt                                                                                                                            <  1:555845/70‑1 (MQ=255)

                                                                           acaATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGAt                                                      <  1:1137257/139‑1 (MQ=255)

                                                                            caATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGa                                                                                                                                   >  1:973652/1‑61 (MQ=255)

                                                                            caATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGa                                                                                                                                   <  2:973652/61‑1 (MQ=255)

                                                                             aaTGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGT‑CTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACg                                                   <  2:45829/139‑1 (MQ=255)

                                                                               tGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGAt                                                               <  1:473615/126‑1 (MQ=255)

                                                                               tGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGAt                                                               >  2:473615/1‑126 (MQ=255)

                                                                                gCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                         >  2:1298268/1‑115 (MQ=255)

                                                                                gCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                         <  1:1298268/115‑1 (MQ=255)

                                                                                gCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTGTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACGATGATCATGTCGATACGtt                                                 <  2:460359/139‑1 (MQ=255)

                                                                                 cccGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAAccc                                                                                                                      >  1:434202/1‑69 (MQ=255)

                                                                                 cccGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTg                                                >  1:111678/1‑139 (MQ=255)

                                                                                 cccGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCGCACTCCCCGATCAGAATCACTTCAAccc                                                                                                                      <  2:434202/69‑1 (MQ=255)

                                                                                  ccgccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGa                                               <  2:403214/139‑1 (MQ=255)

                                                                                     ccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                         <  1:1032860/78‑1 (MQ=255)

                                                                                     ccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                         >  2:1032860/1‑78 (MQ=255)

                                                                                         aCGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                         <  1:373787/74‑1 (MQ=255)

                                                                                         aCGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                         >  2:373787/1‑74 (MQ=255)

                                                                                         aCGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCaa                                        <  2:111678/139‑1 (MQ=255)

                                                                                          cGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATcaaca                                                                    <  1:309955/110‑1 (MQ=255)

                                                                                          cGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATcaaca                                                                    >  2:309955/1‑110 (MQ=255)

                                                                                           gCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATg                                      <  2:1017444/139‑1 (MQ=255)

                                                                                             aTTCGTGTTGACCAGCGGGCATCTTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGAt                                    <  1:991149/139‑1 (MQ=255)

                                                                                              ttCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                         <  1:893694/101‑1 (MQ=255)

                                                                                              ttCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                         >  1:599065/1‑101 (MQ=255)

                                                                                              ttCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                         <  2:599065/101‑1 (MQ=255)

                                                                                              ttCGAGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGCCCTTCCAGGCTGTCCGCAGGAATGCCTtcat                                                                         >  2:893694/1‑101 (MQ=255)

                                                                                                   gtTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATg                              <  1:685600/139‑1 (MQ=255)

                                                                                                       aCCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACg                                                   <  2:766729/114‑1 (MQ=255)

                                                                                                       aCCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACg                                                   >  1:766729/1‑114 (MQ=255)

                                                                                                       aCCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCaa                                        <  2:749210/125‑1 (MQ=255)

                                                                                                       aCCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCaa                                        >  1:749210/1‑125 (MQ=255)

                                                                                                         cAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACt                        >  1:1320712/1‑139 (MQ=255)

                                                                                                           gCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTgg                      <  1:1420153/139‑1 (MQ=255)

                                                                                                              ggCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGa                                                       >  1:598036/1‑103 (MQ=255)

                                                                                                              ggCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGa                                                       <  2:598036/103‑1 (MQ=255)

                                                                                                                cATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAgg                                                                                               <  1:1490188/61‑1 (MQ=255)

                                                                                                                cATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAgg                                                                                               >  2:1490188/1‑61 (MQ=255)

                                                                                                                 aTCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCaa                                        <  1:1470762/115‑1 (MQ=255)

                                                                                                                 aTCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCaa                                        >  2:1470762/1‑115 (MQ=255)

                                                                                                                  tCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCCGCCATTCGCAGTCCTGCCAGGCTATCCGCAGGAACGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGAtt               <  2:929949/139‑1 (MQ=255)

                                                                                                                   ccTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTg              >  2:1350790/1‑139 (MQ=255)

                                                                                                                     tttCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGaa            <  2:995157/139‑1 (MQ=255)

                                                                                                                             tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACg                                                   <  1:1521938/92‑1 (MQ=255)

                                                                                                                             tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACg                                                   >  2:1521938/1‑92 (MQ=255)

                                                                                                                             tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTgg    >  1:1185720/1‑139 (MQ=255)

                                                                                                                               cccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGcc                                                                              >  1:1484793/1‑63 (MQ=255)

                                                                                                                               cccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGcc                                                                              <  2:1484793/63‑1 (MQ=255)

                                                                                                                               cccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGaa  >  2:378197/1‑139 (MQ=255)

GTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAA  >  NC_000913/3277735‑3278000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A