Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	770	51.4	2439994	93.8%	2288714	110.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,282,504	A→G	T177A (ACC→GCC)	agaS →	tagatose‑6‑phosphate ketose/aldose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,282,504	0	A	G	100.0%	192.4 / NA	53	T177A (ACC→GCC)	agaS	tagatose‑6‑phosphate ketose/aldose isomerase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/26); total (27/26)

AATGCTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGAAGGTGTGTTTGGT  >  NC_000913/3282370‑3282635

aaTGCTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTgccac                                                                                                                                 >  2:1211145/1‑139 (MQ=255)

aaTGCTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTgccac                                                                                                                                 >  1:383007/1‑139 (MQ=255)

    cTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCatg                                                                                                                             >  1:748954/1‑139 (MQ=255)

    cTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCatg                                                                                                                             >  2:128271/1‑139 (MQ=255)

     tATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCatga                                                                                                                            <  2:383007/139‑1 (MQ=255)

              ccGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGCTAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCt                                                                                                                   >  2:875754/1‑139 (MQ=255)

              ccGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCt                                                                                                                   >  1:732635/1‑139 (MQ=255)

                 aTCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCATCAGCATTGCCACCATGATGGCCAGCTGcc                                                                                                                >  1:786767/1‑139 (MQ=255)

                    aCCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTcg                                                                                                             <  1:128271/139‑1 (MQ=255)

                     ccTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTcgc                                                                                                            >  1:1011556/1‑139 (MQ=255)

                        gCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGt                                                                                                         <  2:1011556/139‑1 (MQ=255)

                              aaGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGc                                                                                                   >  2:794566/1‑139 (MQ=255)

                              aaGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGc                                                                                                   >  1:130668/1‑139 (MQ=255)

                                   ggCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTg                                                                                              >  2:373718/1‑139 (MQ=255)

                                   ggCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTg                                                                                              <  2:74281/139‑1 (MQ=255)

                                            tACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCa                                                                                     <  2:732635/139‑1 (MQ=255)

                                                    cgcgATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                      >  1:480686/1‑114 (MQ=255)

                                                    cgcgATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                      <  2:480686/114‑1 (MQ=255)

                                                    cgcgATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCaa                                                                             >  2:888889/1‑139 (MQ=255)

                                                        aTCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCt                                                                         >  1:298545/1‑139 (MQ=255)

                                                         tCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                      >  2:986189/1‑109 (MQ=255)

                                                         tCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                      <  1:986189/109‑1 (MQ=255)

                                                              aGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCcgcg                                                                   <  2:130668/139‑1 (MQ=255)

                                                               gCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGa                                                                  <  2:298545/139‑1 (MQ=255)

                                                               gCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGa                                                                  <  1:373718/139‑1 (MQ=255)

                                                                  aTAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTg                                                                                              >  2:591791/1‑108 (MQ=255)

                                                                  aTAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTg                                                                                              <  1:591791/108‑1 (MQ=255)

                                                                          tttGCCCTGCTGATGCCCGCTGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGGCCCGCGGTTCTCGCACCTGAGACGATCAACAGCCAAACCTTCAGCCACGTGGCGGagc                                                       >  1:716155/1‑137 (MQ=255)

                                                                              cccTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTg                                                   <  1:794566/139‑1 (MQ=255)

                                                                                ctgctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGGGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGcc                                                 <  1:1211145/139‑1 (MQ=255)

                                                                                   ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                              <  2:1093112/139‑1 (MQ=255)

                                                                                   ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                              <  1:19191/139‑1 (MQ=255)

                                                                                   ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                              <  1:875754/139‑1 (MQ=255)

                                                                                   ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                              <  1:801089/139‑1 (MQ=255)

                                                                                     gATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCg                                                                                                    >  2:78654/1‑83 (MQ=255)

                                                                                     gATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCg                                                                                                    <  1:78654/83‑1 (MQ=255)

                                                                                        gCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATc                                         <  1:335547/139‑1 (MQ=255)

                                                                                          ccGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTgaga                                                                                           <  1:257566/87‑1 (MQ=255)

                                                                                          ccGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTgaga                                                                                           >  2:257566/1‑87 (MQ=255)

                                                                                           cGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTg                                      >  2:533892/1‑139 (MQ=255)

                                                                                             cAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGAc                                    >  2:519881/1‑139 (MQ=255)

                                                                                             cAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGAc                                    >  2:1098351/1‑139 (MQ=255)

                                                                                                          tCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGAt                       <  1:1114335/139‑1 (MQ=255)

                                                                                                             cgGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCa                                                                                     <  1:53009/74‑1 (MQ=255)

                                                                                                             cgGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCa                                                                                     >  2:53009/1‑74 (MQ=255)

                                                                                                             cgGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCa                                                                                     >  2:1101146/1‑74 (MQ=255)

                                                                                                             cgGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCa                                                                                     <  1:1101146/74‑1 (MQ=255)

                                                                                                                  ttGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                      <  2:416903/52‑1 (MQ=255)

                                                                                                                  ttGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                      >  1:416903/1‑52 (MQ=255)

                                                                                                                   tGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGaa              >  1:323499/1‑139 (MQ=255)

                                                                                                                    gCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGAAg             >  1:828693/1‑139 (MQ=255)

                                                                                                                    gCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGAAg             <  2:828693/139‑1 (MQ=255)

                                                                                                                          aCCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                              >  1:954645/1‑100 (MQ=255)

                                                                                                                          aCCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                              <  2:954645/100‑1 (MQ=255)

                                                                                                                               cagcaTTGCCACAATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGAAGGTGTGTTTGGt  >  1:1047852/1‑139 (MQ=255)

AATGCTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGAAGGTGTGTTTGGT  >  NC_000913/3282370‑3282635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A