Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	772	77.0	3769570	91.6%	3452926	105.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,282,504	A→G	T177A (ACC→GCC)	agaS →	tagatose‑6‑phosphate ketose/aldose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,282,504	0	A	G	100.0%	206.1 / NA	59	T177A (ACC→GCC)	agaS	tagatose‑6‑phosphate ketose/aldose isomerase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (30/29); total (30/29)

AATGCTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGAAGGTGTGTTTGGTTACG  >  NC_000913/3282370‑3282639

aaTGCTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTgccac                                                                                                                                     >  2:330467/1‑139 (MQ=255)

  tGCTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCGCGATCGCGGCTTTGCGATGACCAGCAGCATTGccacca                                                                                                                                   <  2:786415/139‑1 (MQ=255)

             gCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATTGCCAGc                                                                                                                        >  2:1004422/1‑139 (MQ=255)

             gCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGc                                                                                                                        >  1:1667963/1‑139 (MQ=255)

                 aTCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGcc                                                                                                                    >  2:62309/1‑139 (MQ=255)

                      cTGCAACGAAGCGGGCGCTCTTTACCAACACGCACTCAACAGCGATAACCCGTTTGCCCTTCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCTTGATGGCCAGCTGCCTcgcg                                                                                                               <  1:1004422/139‑1 (MQ=255)

                        gCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGt                                                                                                             >  1:844425/1‑139 (MQ=255)

                           aCGAAGCGGGCGCTCTTTACCAAAACGCGATGAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGAAGAAACGCACGATCGCGGCTTTGCGATAACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                          <  1:501460/139‑1 (MQ=255)

                           aCGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                          <  1:486921/139‑1 (MQ=255)

                              aaGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGc                                                                                                       >  2:1413819/1‑139 (MQ=255)

                                  gggCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCt                                                                                                   <  2:1714097/139‑1 (MQ=255)

                                   ggCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTACCTCGCGGTTTTCGCACCTg                                                                                                  >  2:1558391/1‑139 (MQ=255)

                                       ctctTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGAc                                                                                              <  1:62309/139‑1 (MQ=255)

                                           ttACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATc                                                                                          <  2:580638/139‑1 (MQ=255)

                                           ttACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATc                                                                                          <  2:1314794/139‑1 (MQ=255)

                                                  aaCGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAg                                                                                                                         <  2:1470121/101‑1 (MQ=255)

                                                  aaCGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAg                                                                                                                         >  1:1470121/1‑101 (MQ=255)

                                                  aaCGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATc                                                                                          >  1:800652/1‑132 (MQ=255)

                                                  aaCGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATc                                                                                          <  2:800652/132‑1 (MQ=255)

                                                  aaCGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACATcc                                                                                   >  1:1344277/1‑139 (MQ=255)

                                                   aCGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTACCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCa                                                                                  <  1:1558391/139‑1 (MQ=255)

                                                    cgcgATCAACAGCGATAACGCGTTTGTCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCaa                                                                                 >  2:686042/1‑139 (MQ=255)

                                                    cgcgATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCaa                                                                                 >  2:1044826/1‑139 (MQ=255)

                                                            aCAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCcg                                                                         <  1:1413819/139‑1 (MQ=255)

                                                               gCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGa                                                                      >  1:1740824/1‑139 (MQ=255)

                                                                   tAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                          >  2:785383/1‑99 (MQ=255)

                                                                   tAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                          <  1:785383/99‑1 (MQ=255)

                                                                   tAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTg                                                                  >  1:12310/1‑139 (MQ=255)

                                                                   tAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTCGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTg                                                                  >  2:1705707/1‑139 (MQ=255)

                                                                     aCGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTcgcg                                                                                                               >  1:850139/1‑92 (MQ=255)

                                                                     aCGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTcgcg                                                                                                               <  2:850139/92‑1 (MQ=255)

                                                                     aCGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGc                                                                <  2:694203/139‑1 (MQ=255)

                                                                          tttGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATc                                                           >  2:1296908/1‑139 (MQ=255)

                                                                             gCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCg                                                                                                        >  1:1430596/1‑91 (MQ=255)

                                                                             gCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCg                                                                                                        <  2:1430596/91‑1 (MQ=255)

                                                                                   ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGc                                                                <  1:1666997/125‑1 (MQ=255)

                                                                                   ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGc                                                                >  2:1666997/1‑125 (MQ=255)

                                                                                   ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                                  <  1:1296908/139‑1 (MQ=255)

                                                                                   ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                                  >  2:201963/1‑139 (MQ=255)

                                                                                   ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                                  <  1:330467/139‑1 (MQ=255)

                                                                                   ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                                  <  2:1667963/139‑1 (MQ=255)

                                                                                       tGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGt                                                                                                             <  1:1751034/76‑1 (MQ=255)

                                                                                       tGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGt                                                                                                             >  2:1751034/1‑76 (MQ=255)

                                                                                             cAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGAc                                        >  2:557790/1‑139 (MQ=255)

                                                                                                  aCGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCAc                                   >  1:1233978/1‑139 (MQ=255)

                                                                                                          tCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                                  <  2:1565866/116‑1 (MQ=255)

                                                                                                          tCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGACAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                                  >  1:1565866/1‑116 (MQ=255)

                                                                                                             cgGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCC‑GGCGATCCTGACCTCACTGGGCGATTTCa                       >  1:455341/1‑139 (MQ=255)

                                                                                                                cTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGc                     >  2:894867/1‑139 (MQ=255)

                                                                                                                   tGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                          <  2:1027445/51‑1 (MQ=255)

                                                                                                                   tGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                          >  1:1027445/1‑51 (MQ=255)

                                                                                                                    gCGATGACCAGGAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGAt                                              <  1:1182416/110‑1 (MQ=255)

                                                                                                                    gCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                          >  2:1799213/1‑50 (MQ=255)

                                                                                                                    gCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                          <  1:1799213/50‑1 (MQ=255)

                                                                                                                    gCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGAt                                              >  2:1182416/1‑110 (MQ=255)

                                                                                                                          aCCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCC‑GGCGATCCTGACCTCACTGGGCGATTTCAGCGAAGGTGTGtt          <  2:455341/139‑1 (MQ=255)

                                                                                                                               cagcaTTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCa                                                                                                      >  2:1690544/1‑43 (MQ=255)

                                                                                                                               cagcaTTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCa                                                                                                      <  1:1690544/43‑1 (MQ=255)

                                                                                                                                 gcaTTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGAAGGTGTGTTTGGTTa    <  1:557790/139‑1 (MQ=255)

                                                                                                                                   aTTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGAAGGTGTGTTTGGTTACg  <  1:686042/139‑1 (MQ=255)

AATGCTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGAAGGTGTGTTTGGTTACG  >  NC_000913/3282370‑3282639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A