Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I1 R1
|
760 |
58.2 |
3022526 |
90.8% |
2744453 |
102.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,285,690 |
C→T |
V71V (GTC→GTT) |
agaD → |
N‑acetylgalactosamine‑specific enzyme IID component of PTS |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,285,690 | 0 | C | T | 100.0%
| 168.7
/ NA
| 54 | V71V (GTC→GTT) | agaD | N‑acetylgalactosamine‑specific enzyme IID component of PTS |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (29/25); total (29/25) |
CGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACT > NC_000913/3285555‑3285816
|
cGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGa > 2:1322465/1‑139 (MQ=255)
cGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGa > 1:1470546/1‑139 (MQ=255)
gAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGa > 2:1200162/1‑138 (MQ=255)
gAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGa < 1:1200162/138‑1 (MQ=255)
gAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGAt < 1:373258/139‑1 (MQ=255)
ggcgggcgGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATgggg > 1:1493671/1‑139 (MQ=255)
aCCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGa < 1:1322465/139‑1 (MQ=255)
cTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATg < 2:1493671/139‑1 (MQ=255)
ggCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGAt > 2:302637/1‑117 (MQ=255)
ggCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGAt < 1:302637/117‑1 (MQ=255)
gCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGa < 1:414259/133‑1 (MQ=255)
gCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGa > 2:414259/1‑133 (MQ=255)
tGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGaaaa > 2:1070365/1‑139 (MQ=255)
ccGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATg > 1:18466/1‑108 (MQ=255)
ccGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATg < 2:18466/108‑1 (MQ=255)
cGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATg > 2:845069/1‑125 (MQ=255)
cGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATg < 1:845069/125‑1 (MQ=255)
tCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGaaaa > 1:1167486/1‑139 (MQ=255)
ccTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATggg < 1:1233888/105‑1 (MQ=255)
ccTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATggg > 2:1233888/1‑105 (MQ=255)
tGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACcg < 2:1048264/139‑1 (MQ=255)
tGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACcg > 1:1048264/1‑139 (MQ=255)
aGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGacac < 2:1167486/139‑1 (MQ=255)
gacgacAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGgaaga < 2:116756/107‑1 (MQ=255)
gacgacAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGgaaga > 1:116756/1‑107 (MQ=255)
acgacAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGa < 2:458104/99‑1 (MQ=255)
acgacAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGa > 1:458104/1‑99 (MQ=255)
gggCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCa < 1:1070365/139‑1 (MQ=255)
aGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTg > 2:263686/1‑139 (MQ=255)
cggcgATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTAGTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAGGGCCTCAAAGTGACACTGTTTGGccg > 2:331415/1‑138 (MQ=255)
gATAACCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGa < 1:331415/139‑1 (MQ=255)
aCCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCa > 2:163400/1‑113 (MQ=255)
aCCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCa < 1:163400/113‑1 (MQ=255)
aCCTCGAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTgg < 2:1470546/139‑1 (MQ=255)
gAATTTATTAATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGgcgatg < 1:263686/139‑1 (MQ=255)
attaATACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGAttt > 1:1266915/1‑139 (MQ=255)
tACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAAc > 2:7323/1‑67 (MQ=255)
tACCCACCCGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAAc < 1:7323/67‑1 (MQ=255)
acccacccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGac < 1:268782/72‑1 (MQ=255)
acccacccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGac > 2:268782/1‑72 (MQ=255)
gcccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGtt > 1:225049/2‑136 (MQ=255)
gcccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGtt < 2:225049/135‑1 (MQ=255)
acccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGc < 2:165674/109‑1 (MQ=255)
acccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGc > 1:165674/1‑109 (MQ=255)
acccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGAttt > 2:1393854/1‑129 (MQ=255)
acccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGAttt < 1:1393854/129‑1 (MQ=255)
acccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTAc > 1:657282/1‑139 (MQ=255)
acccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTAc > 2:452345/1‑139 (MQ=255)
cccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGc < 2:469331/108‑1 (MQ=255)
cccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGc > 1:469331/1‑108 (MQ=255)
cccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACt > 2:621697/1‑139 (MQ=255)
cccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTCGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACt > 2:903156/1‑139 (MQ=255)
ccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCa > 2:306135/1‑90 (MQ=255)
ccGAATCTGGTTGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCa < 1:306135/90‑1 (MQ=255)
|
CGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGAAAAAGGAGAAAACCGCGACACCATTAAAGGCCTCAAAGTGGCACTGTTTGGCCCAATCGCCGGGATTGGCGATGCGATTTTCTGGTTTACT > NC_000913/3285555‑3285816
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
