Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I1 R1
|
760 |
58.2 |
3022526 |
90.8% |
2744453 |
102.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,854,733 |
(T)5→4 |
coding (396/2079 nt) |
fhlA → |
formate hydrogenlyase transcriptional activator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,854,729 | 0 | T | . | 100.0%
| 94.2
/ NA
| 24 | coding (392/2079 nt) | fhlA | formate hydrogenlyase transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (12/12); total (12/12) |
TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCG > NC_000913/2854597‑2854846
|
ttttGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAttttggt < 2:1126235/139‑4 (MQ=255)
gcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGaa < 2:1159506/139‑1 (MQ=255)
tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTAt > 2:684301/1‑139 (MQ=255)
aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCtgt > 1:186053/1‑112 (MQ=255)
aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCtgt < 2:186053/112‑1 (MQ=255)
gaagaaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTg < 2:683344/139‑1 (MQ=255)
gtgAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaaaaa < 1:373962/139‑1 (MQ=255)
ccTGGCCGCAGCTGGACGCAGGTGGGCTCTACCCAAAATTTGGTCACTAATGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAAtt > 2:975736/1‑99 (MQ=255)
ccTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGCAAGGGCATA‑TTTTGGTGGCTGTGAAtt < 1:975736/99‑1 (MQ=255)
ggCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAAt > 1:1218873/1‑139 (MQ=255)
gCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATc < 2:1218873/139‑1 (MQ=255)
ccGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTGATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCg < 2:1066180/139‑1 (MQ=255)
aGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTg > 2:1439313/1‑139 (MQ=255)
gCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGc > 1:1361795/1‑139 (MQ=255)
taCCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑GTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGt > 1:1130695/1‑139 (MQ=255)
tGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCa < 1:1439313/139‑1 (MQ=255)
ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaaa > 1:669348/1‑90 (MQ=255)
ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaaa < 2:669348/90‑1 (MQ=255)
ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc < 1:684301/139‑1 (MQ=255)
ccTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt < 1:257044/63‑1 (MQ=255)
ccTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt > 2:257044/1‑63 (MQ=255)
aTGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAATGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCagag > 2:1079146/1‑139 (MQ=255)
tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc > 2:1057023/1‑139 (MQ=255)
gCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGcc < 2:1361795/139‑1 (MQ=255)
ccACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcg > 1:386756/1‑139 (MQ=255)
|
TTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCG > NC_000913/2854597‑2854846
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
