Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I2 R1
|
766 |
55.6 |
2631458 |
94.7% |
2491990 |
109.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,854,733 |
(T)5→4 |
coding (396/2079 nt) |
fhlA → |
formate hydrogenlyase transcriptional activator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,854,729 | 0 | T | . | 100.0%
| 198.7
/ NA
| 48 | coding (392/2079 nt) | fhlA | formate hydrogenlyase transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (21/27); total (21/27) |
AGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTC > NC_000913/2854593‑2854850
|
agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAttt > 2:438061/1‑139 (MQ=255)
tGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGc < 2:1191557/139‑1 (MQ=255)
gTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCt < 1:851882/139‑1 (MQ=255)
tCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCtg < 2:1247436/139‑1 (MQ=255)
gATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTAt < 1:260065/139‑1 (MQ=255)
tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTAt > 1:1239070/1‑139 (MQ=255)
cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGa > 2:130683/1‑139 (MQ=255)
cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGa > 1:1234113/1‑139 (MQ=255)
ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAttttggt > 2:68967/1‑111 (MQ=255)
ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAttttggt < 1:68967/114‑4 (MQ=255)
ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACg < 2:921483/139‑1 (MQ=255)
ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACg > 1:1154419/1‑139 (MQ=255)
gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt < 2:1234113/139‑1 (MQ=255)
gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt < 1:967917/139‑1 (MQ=255)
tgAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAttttgg > 2:963844/1‑90 (MQ=255)
tgAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATAttttgg < 1:963844/92‑3 (MQ=255)
ccTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt > 2:1079378/1‑115 (MQ=255)
ccTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt < 1:1079378/115‑1 (MQ=255)
ggCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAAt > 2:770851/1‑139 (MQ=255)
gCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGc > 2:910176/1‑139 (MQ=255)
cTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCa < 2:1154419/139‑1 (MQ=255)
gACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAAc > 2:288481/1‑139 (MQ=255)
aCGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACa < 1:130683/139‑1 (MQ=255)
aGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt > 1:757724/1‑96 (MQ=255)
aGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt < 2:757724/96‑1 (MQ=255)
ggTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGTAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTGATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTAc < 2:1239070/139‑1 (MQ=255)
ggCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAg < 1:288481/139‑1 (MQ=255)
cTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGAt > 1:780518/1‑139 (MQ=255)
ttGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTgtcgtc < 1:438061/139‑1 (MQ=255)
tGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCa < 1:910176/139‑1 (MQ=255)
ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc > 1:525542/1‑139 (MQ=255)
ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc < 1:883666/139‑1 (MQ=255)
ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTACTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc < 2:806001/139‑1 (MQ=255)
tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt > 1:613594/1‑58 (MQ=255)
tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt < 2:613594/58‑1 (MQ=255)
tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGccc < 2:939682/63‑1 (MQ=255)
tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGccc > 1:939682/1‑63 (MQ=255)
tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGt < 1:365679/88‑1 (MQ=255)
tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc > 1:685766/1‑139 (MQ=255)
tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc > 1:418182/1‑139 (MQ=255)
tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc > 2:731447/1‑139 (MQ=255)
tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc > 2:975530/1‑139 (MQ=255)
tGCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCATTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGt > 2:365679/1‑88 (MQ=255)
gCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGcc < 2:1127337/139‑1 (MQ=255)
gCCACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGcc < 1:975530/139‑1 (MQ=255)
cACTGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcgc < 2:685766/139‑1 (MQ=255)
tGGCGGCGGAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGcgtc < 2:988339/139‑1 (MQ=255)
ggcggAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt < 2:829513/48‑1 (MQ=255)
ggcggAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt > 1:829513/1‑48 (MQ=255)
ggcggAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc < 2:846752/113‑1 (MQ=255)
ggcggAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc > 1:846752/1‑113 (MQ=255)
ggAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt < 2:619260/45‑1 (MQ=255)
ggAAGGGCATA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGAt > 1:619260/1‑45 (MQ=255)
|
AGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTC > NC_000913/2854593‑2854850
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
