Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I1 R1 760 58.2 3022526 90.8% 2744453 102.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,059,293 T→C G11G (GGA→GGG)  rpiA ← ribose 5‑phosphate isomerase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,059,2930TC100.0% 242.5 / NA 68G11G (GGA→GGG) rpiAribose 5‑phosphate isomerase, constitutive
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/34);  total (34/34)

TGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATAAAATATC  >  NC_000913/3059167‑3059427
                                                                                                                              |                                                                                                                                      
tGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCAAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCtttt                                                                                                                            <  2:860708/139‑1 (MQ=255)
           cGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATc                                                                                                                 <  1:587533/139‑1 (MQ=255)
             gCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCt                                                                                                               <  1:1460841/139‑1 (MQ=255)
               cccTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCGACCCTACTGCTTTTTTCAATTCATCCt                                                                                                               <  2:898737/137‑1 (MQ=255)
               cccTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCt                                                                                                               >  1:898737/1‑137 (MQ=255)
                           gCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGtt                                                                                                 >  2:1342371/1‑139 (MQ=255)
                            ccTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGttt                                                                                                >  1:98202/1‑139 (MQ=255)
                                        ccGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTAt                                                                                    >  1:972214/1‑139 (MQ=255)
                                           aGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGaa                                                                                 >  1:1212038/1‑139 (MQ=255)
                                              gcgTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAAtt                                                                              >  2:579386/1‑139 (MQ=255)
                                                gTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTc                                                                            >  2:150275/1‑139 (MQ=255)
                                                gTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTc                                                                            >  1:684683/1‑139 (MQ=255)
                                                  cAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                          <  2:98202/139‑1 (MQ=255)
                                                     tAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGc                                                                                             >  2:736523/1‑117 (MQ=255)
                                                     tAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGc                                                                                             <  1:736523/117‑1 (MQ=255)
                                                         gtgtGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCAtt                                                                   <  2:972214/139‑1 (MQ=255)
                                                         gtgtGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCAtt                                                                   <  1:1342371/139‑1 (MQ=255)
                                                            tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                          <  2:1298343/113‑1 (MQ=255)
                                                            tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                          >  1:1298343/1‑113 (MQ=255)
                                                            tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                          <  1:525882/113‑1 (MQ=255)
                                                            tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                          >  2:525882/1‑113 (MQ=255)
                                                            tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                          >  2:693489/1‑129 (MQ=255)
                                                            tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                          <  1:693489/129‑1 (MQ=255)
                                                            tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATtata                                                                >  1:1355509/1‑139 (MQ=255)
                                                                      gAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATg                                                                                                       <  2:859001/90‑1 (MQ=255)
                                                                      gAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATg                                                                                                       >  1:859001/1‑90 (MQ=255)
                                                                                cTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGa                                            >  1:1095650/1‑139 (MQ=255)
                                                                                cTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGa                                            <  2:684683/139‑1 (MQ=255)
                                                                                 tACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGAc                                           <  2:434328/139‑1 (MQ=255)
                                                                                  acacCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTc                                                                            >  2:654581/1‑105 (MQ=255)
                                                                                  acacCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTc                                                                            <  1:654581/105‑1 (MQ=255)
                                                                                   cacCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCttttt                                                                                                                           <  1:1466911/57‑1 (MQ=255)
                                                                                   cacCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCttttt                                                                                                                           >  2:1466911/1‑57 (MQ=255)
                                                                                   cacCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGAccc                                         <  2:938102/139‑1 (MQ=255)
                                                                                   cacCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCAGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGAccc                                         >  1:938102/1‑139 (MQ=255)
                                                                                       aacaaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGc                                                                                             >  1:1294736/1‑83 (MQ=255)
                                                                                       aacaaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGc                                                                                             <  2:1294736/83‑1 (MQ=255)
                                                                                        acaaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATcc                                                                                                                <  2:207283/63‑1 (MQ=255)
                                                                                        acaaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATcc                                                                                                                >  1:207283/1‑63 (MQ=255)
                                                                                          aaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCt                                                                                                               <  2:29672/62‑1 (MQ=255)
                                                                                          aaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCt                                                                                                               >  1:29672/1‑62 (MQ=255)
                                                                                              gTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAAtt                                                                                                                     >  1:741491/1‑52 (MQ=255)
                                                                                              gTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAAtt                                                                                                                     <  2:741491/52‑1 (MQ=255)
                                                                                               tGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                          <  2:1267214/94‑1 (MQ=255)
                                                                                               tGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                          >  1:1267214/1‑94 (MQ=255)
                                                                                               tGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGcc                             <  1:579386/139‑1 (MQ=255)
                                                                                               ggCCCGGCTGAACATACTGAAGTGTCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGcg                             <  2:1095650/138‑2 (MQ=255)
                                                                                                gCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCcg                            >  1:329623/1‑139 (MQ=255)
                                                                                                 ccGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCcgc                           <  2:1355509/139‑1 (MQ=255)
                                                                                                         aaCATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGtt                     <  2:1314340/137‑1 (MQ=255)
                                                                                                         aaCATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGtt                     >  1:1314340/1‑137 (MQ=255)
                                                                                                          aCATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAAc                  <  2:1212038/139‑1 (MQ=255)
                                                                                                              aCTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCAt              <  1:150275/139‑1 (MQ=255)
                                                                                                                   aGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                          <  1:256204/58‑1 (MQ=255)
                                                                                                                   aGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtg                                                                                          >  2:256204/1‑58 (MQ=255)
                                                                                                                       ccgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtgtg                                                                                        >  2:1277017/1‑56 (MQ=255)
                                                                                                                       ccgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCtgtg                                                                                        <  1:1277017/56‑1 (MQ=255)
                                                                                                                       ccgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCt                                                                                           <  1:1130681/53‑1 (MQ=255)
                                                                                                                       ccgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCt                                                                                           >  2:1130681/1‑53 (MQ=255)
                                                                                                                       ccgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGa                                            <  2:660219/100‑1 (MQ=255)
                                                                                                                       ccgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGa                                            >  1:660219/1‑100 (MQ=255)
                                                                                                                       ccgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATAAAat     >  2:384364/1‑139 (MQ=255)
                                                                                                                       ccgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATAAAat     >  1:977359/1‑139 (MQ=255)
                                                                                                                        cgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGTAAGCcgc                           <  1:922006/116‑1 (MQ=255)
                                                                                                                        cgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCcgc                           >  2:922006/1‑116 (MQ=255)
                                                                                                                          cccACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCcg                            >  1:1142565/1‑113 (MQ=255)
                                                                                                                          cccACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCcg                            <  2:1142565/113‑1 (MQ=255)
                                                                                                                          cccACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATAAAATATc  >  1:392987/1‑139 (MQ=255)
                                                                                                                              |                                                                                                                                      
TGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATAAAATATC  >  NC_000913/3059167‑3059427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A