Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,059,293	T→C	G11G (GGA→GGG)	rpiA ←	ribose 5‑phosphate isomerase, constitutive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,059,293	0	T	C	100.0%	246.3 / NA	68	G11G (GGA→GGG)	rpiA	ribose 5‑phosphate isomerase, constitutive
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (33/35); total (33/35)

GAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATAAAATATCG  >  NC_000913/3059159‑3059428

gAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTa                                                                                                                                     <  2:1586383/139‑1 (MQ=255)

gAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTa                                                                                                                                     <  2:470618/139‑1 (MQ=255)

        tGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCtttt                                                                                                                             >  2:831365/1‑139 (MQ=255)

         gAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCttttt                                                                                                                            <  1:831365/139‑1 (MQ=255)

               gAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCttttt                                                                                                                            >  1:371574/1‑133 (MQ=255)

               gAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCttttt                                                                                                                            <  2:371574/133‑1 (MQ=255)

                   cGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATc                                                                                                                  <  1:314407/139‑1 (MQ=255)

                    ggCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATcc                                                                                                                 >  2:163610/1‑139 (MQ=255)

                                   gCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGtt                                                                                                  >  1:1415266/1‑139 (MQ=255)

                                       ttCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGc                                                                                              <  2:332297/139‑1 (MQ=255)

                                       ttCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGc                                                                                              <  1:809905/139‑1 (MQ=255)

                                        tCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGcc                                                                                             >  1:763659/1‑139 (MQ=255)

                                        tCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGcc                                                                                             >  1:1161762/1‑139 (MQ=255)

                                        tCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGcc                                                                                             <  1:163610/139‑1 (MQ=255)

                                                   aGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATcc                                                                                                                 <  1:386203/108‑1 (MQ=255)

                                                   aGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATcc                                                                                                                 >  2:386203/1‑108 (MQ=255)

                                                   aGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGaa                                                                                  >  1:86340/1‑139 (MQ=255)

                                                   aGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGaa                                                                                  >  2:30006/1‑139 (MQ=255)

                                                   aGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGaa                                                                                  >  2:1627853/1‑139 (MQ=255)

                                                    gcgcGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGaaa                                                                                 >  1:853412/1‑139 (MQ=255)

                                                         tCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTca                                                                            <  1:1347203/139‑1 (MQ=255)

                                                               aaGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCa                                                                      <  1:1351762/139‑1 (MQ=255)

                                                                  tgtgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCa                                                                      >  1:323341/1‑136 (MQ=255)

                                                                  tgtgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCa                                                                      <  2:323341/136‑1 (MQ=255)

                                                                    tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCTTCCTGAGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                         <  1:1107572/131‑1 (MQ=255)

                                                                    tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTa                                                                                      <  1:937888/118‑1 (MQ=255)

                                                                    tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTa                                                                                      >  2:937888/1‑118 (MQ=255)

                                                                    tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                         >  2:1107572/1‑131 (MQ=255)

                                                                    tgCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATtata                                                                 <  1:1061002/139‑1 (MQ=255)

                                                                              gAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                         >  1:434762/1‑121 (MQ=255)

                                                                              gAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                         <  2:434762/121‑1 (MQ=255)

                                                                                   tGTACCTACACCAACAATGGTGCGGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATg                                                                                                        <  2:1068954/85‑1 (MQ=255)

                                                                                   tGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATg                                                                                                        >  1:1068954/1‑85 (MQ=255)

                                                                                   tGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATtata                                                                 <  1:899241/124‑1 (MQ=255)

                                                                                   tGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATtata                                                                 >  2:899241/1‑124 (MQ=255)

                                                                                   tGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGAt                                                  <  2:763659/139‑1 (MQ=255)

                                                                                      aCCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATtata                                                                 >  1:715669/1‑121 (MQ=255)

                                                                                      aCCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATtata                                                                 <  2:715669/121‑1 (MQ=255)

                                                                                       ccTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAg                                              <  2:853412/139‑1 (MQ=255)

                                                                                            acCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGAcccc                                         >  2:1354902/1‑139 (MQ=255)

                                                                                               aacaaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGtt                                      >  1:986409/1‑139 (MQ=255)

                                                                                                acaaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGAATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTg                                     >  1:1050233/1‑139 (MQ=255)

                                                                                                 caaTGGTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATGCAAAAAAAGCGATTCAGACCCCGTTgg                                    >  2:954948/1‑139 (MQ=255)

                                                                                                      gTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                         >  1:425777/1‑97 (MQ=255)

                                                                                                      gTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                         <  2:425777/97‑1 (MQ=255)

                                                                                                      gTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGAc                                            >  1:1633505/1‑126 (MQ=255)

                                                                                                      gTGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGAc                                            <  2:1633505/126‑1 (MQ=255)

                                                                                                       tGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcaca                                                                          >  1:238879/1‑95 (MQ=255)

                                                                                                       tGCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcaca                                                                          <  2:238879/95‑1 (MQ=255)

                                                                                                        gCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCcg                             <  2:1161762/139‑1 (MQ=255)

                                                                                                        gCCGGGCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCcg                             <  1:954948/139‑1 (MQ=255)

                                                                                                            ggCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                         >  2:1587445/1‑91 (MQ=255)

                                                                                                            ggCTGAACATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                         <  1:1587445/91‑1 (MQ=255)

                                                                                                                 aaCATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                           >  1:82707/1‑84 (MQ=255)

                                                                                                                 aaCATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcac                                                                           <  2:82707/84‑1 (MQ=255)

                                                                                                                 aaCATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTaa                    >  2:510482/1‑139 (MQ=255)

                                                                                                                   cATACTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACt                  <  1:510482/139‑1 (MQ=255)

                                                                                                                      aCTGAAGTGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCAt               <  2:86340/139‑1 (MQ=255)

                                                                                                                             tGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGt                                                                                                   <  2:1209916/48‑1 (MQ=255)

                                                                                                                             tGCCGCCCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGt                                                                                                   >  1:1209916/1‑48 (MQ=255)

                                                                                                                              gccgccCACCCTACTGCTTTTTTCAATTCTTCCTGCGTCATGATCGTTTCGCCTGTAGTATGAAATTTcaca                                                                          <  1:21270/72‑1 (MQ=255)

                                                                                                                              gccgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcaca                                                                          >  2:21270/1‑72 (MQ=255)

                                                                                                                               ccgccCACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATAAAat      >  2:1031980/1‑139 (MQ=255)

                                                                                                                                  cccACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcaca                                                                          >  2:1361386/1‑68 (MQ=255)

                                                                                                                                  cccACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTcaca                                                                          <  1:1361386/68‑1 (MQ=255)

                                                                                                                                  cccACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                         >  2:103510/1‑69 (MQ=255)

                                                                                                                                  cccACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCacac                                                                         <  1:103510/69‑1 (MQ=255)

                                                                                                                                   ccACCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATAAAATATcg  <  2:1050233/139‑1 (MQ=255)

GAAGCATCTGAACTGGAAACGGCCCCTTCAATCTGGCCTTTCATTGTACCGAGCGCGTCAATAAAGTGTGCGGCGGTGGAACCTGTACCTACACCAACAATGGTGCCGGGCTGAACATACTGAAGTGCCGCCCATCCTACTGCTTTTTTCAATTCATCCTGCGTCATGATCGTTTCGCCTGTGGTATGAAATTTCACACGCATTATATACAAAAAAAGCGATTCAGACCCCGTTGGCAAGCCGCGTGGTTAACTCATCCATAAAATATCG  >  NC_000913/3059159‑3059428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A