Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I2 R1	758	59.3	2864096	93.9%	2689386	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,134,823	G→A	231 (TAG→TAA)	yghT →	putative ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,134,823	0	G	A	100.0%	86.0 / NA	28	231 (TAG→TAA)	yghT	putative ATP‑binding protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/14); total (14/14)

GTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGC  >  NC_000913/3134717‑3134946

gtggtgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCgg                                                                                            >  1:1383903/1‑139 (MQ=255)

     gCACAACTGGTTGATGTCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAtt                                                                                       >  1:410022/1‑139 (MQ=255)

                tGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGt                                                                            <  2:316180/139‑1 (MQ=255)

                   tgtCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTaa                                                                         <  2:410022/137‑1 (MQ=255)

                        aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                          <  1:1278523/101‑1 (MQ=255)

                        aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                          >  2:1278523/1‑101 (MQ=255)

                            cAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGa                                                                                                         <  1:1041722/98‑1 (MQ=255)

                            cAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGa                                                                                                         >  2:1041722/1‑98 (MQ=255)

                                                   tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                          <  1:1289012/74‑1 (MQ=255)

                                                   tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                          >  2:1289012/1‑74 (MQ=255)

                                                         aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                          >  2:399354/1‑68 (MQ=255)

                                                         aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                          <  2:359121/68‑1 (MQ=255)

                                                         aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                          >  1:359121/1‑68 (MQ=255)

                                                         aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                                          <  1:399354/68‑1 (MQ=255)

                                                         aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                                   >  2:282245/1‑139 (MQ=255)

                                                               aaaaaGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGa                                                                                                >  1:1150521/1‑72 (MQ=255)

                                                               aaaaaGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGa                                                                                                <  2:1150521/72‑1 (MQ=255)

                                                                       aTTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTaa                                                                         <  2:163395/87‑1 (MQ=255)

                                                                       aTTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTaa                                                                         >  1:163395/1‑87 (MQ=255)

                                                                         tACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgt                   <  1:282245/139‑1 (MQ=255)

                                                                                       ccGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCa     <  2:497831/139‑1 (MQ=255)

                                                                                           cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGAc                                                                                               >  2:1270396/5‑46 (MQ=255)

                                                                                           cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGAc                                                                                               <  1:1270396/42‑1 (MQ=255)

                                                                                           cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCgg                                                                                            >  2:1369307/5‑49 (MQ=255)

                                                                                           cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCgg                                                                                            <  1:1369307/45‑1 (MQ=255)

                                                                                           cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCa                                                               <  1:462166/74‑1 (MQ=255)

                                                                                           cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCa                                                               >  2:462166/5‑78 (MQ=255)

                                                                                           cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc  >  2:1185299/5‑139 (MQ=255)

GTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGC  >  NC_000913/3134717‑3134946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A