Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I1 R1	770	68.5	3470224	91.7%	3182195	103.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,134,823	G→A	231 (TAG→TAA)	yghT →	putative ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,134,823	0	G	A	100.0%	195.5 / NA	61	231 (TAG→TAA)	yghT	putative ATP‑binding protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (31/30); total (31/30)

TTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAA  >  NC_000913/3134690‑3134931

ttGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCaaaa                                                                                                        >  1:1261732/1‑139 (MQ=255)

ttGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCaaaa                                                                                                        >  1:993232/1‑139 (MQ=255)

             acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                           <  2:1561995/139‑1 (MQ=255)

             acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                           <  2:1261732/139‑1 (MQ=255)

             acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGATTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                           <  2:993232/139‑1 (MQ=255)

                   aaCCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGcc                                                                                     >  1:1192295/1‑139 (MQ=255)

                               tgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                           <  2:352412/121‑1 (MQ=255)

                               tgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                           >  1:352412/1‑121 (MQ=255)

                                      cTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                  >  2:602332/1‑139 (MQ=255)

                                      cTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                  >  2:1184429/1‑139 (MQ=255)

                                      cTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                  <  1:602332/139‑1 (MQ=255)

                                         gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                               >  2:123082/1‑139 (MQ=255)

                                              tatCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                  <  2:357492/131‑1 (MQ=255)

                                              tatCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                  >  1:357492/1‑131 (MQ=255)

                                                 cGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATc                                                       <  2:45590/139‑1 (MQ=255)

                                                        gaTCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCa                                                >  2:1082729/2‑139 (MQ=255)

                                                         aTCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                  <  2:666736/120‑1 (MQ=255)

                                                         aTCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                  >  1:666736/1‑120 (MQ=255)

                                                           cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                             <  1:123082/139‑1 (MQ=255)

                                                              ccACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                  <  1:1398652/115‑1 (MQ=255)

                                                              ccACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                  >  2:1398652/1‑115 (MQ=255)

                                                                aCTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATc                                        >  1:528578/1‑139 (MQ=255)

                                                                  tGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCa                                      >  2:1419971/1‑139 (MQ=255)

                                                                           tgtTGGTTGATGCAGAATAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGc                                                                           >  2:30901/1‑93 (MQ=255)

                                                                           tgtTGGTTGATGCAGAAAAAGCGATTACGGATTGTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGc                                                                           <  1:30901/93‑1 (MQ=255)

                                                                            gttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCggg                                                                            >  2:221599/1‑91 (MQ=255)

                                                                            gttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCggg                                                                            <  1:221599/91‑1 (MQ=255)

                                                                            gttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCgg                                                                             >  2:441957/1‑90 (MQ=255)

                                                                            gttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCgg                                                                             <  1:441957/90‑1 (MQ=255)

                                                                             ttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGcctg                                                                                           >  1:1061340/1‑73 (MQ=255)

                                                                             ttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                           <  2:1061340/75‑1 (MQ=255)

                                                                              tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccacc                          >  2:694719/1‑139 (MQ=255)

                                                                               ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATc                                                                                                            <  2:1637156/56‑1 (MQ=255)

                                                                               ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATc                                                                                                            >  1:1637156/1‑56 (MQ=255)

                                                                                    aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                           <  2:156156/68‑1 (MQ=255)

                                                                                    aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                           >  1:156156/1‑68 (MQ=255)

                                                                                    aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGc                                                                                      <  2:1363530/73‑1 (MQ=255)

                                                                                    aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGc                                                                                      >  1:1363530/1‑73 (MQ=255)

                                                                                    aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGc                                                                           >  2:1394263/1‑84 (MQ=255)

                                                                                    aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGc                                                                           <  1:1394263/84‑1 (MQ=255)

                                                                                    aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                    >  1:898396/1‑139 (MQ=255)

                                                                                         gAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                  <  1:401516/104‑1 (MQ=255)

                                                                                         gAAAAAGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGCTAAATCAACTg                                                  >  2:401516/1‑104 (MQ=255)

                                                                                           aaaaGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAAtt             <  2:1192295/139‑1 (MQ=255)

                                                                                             aaGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgc           <  2:898396/139‑1 (MQ=255)

                                                                                              aGCGATTACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgcc          <  1:138910/139‑1 (MQ=255)

                                                                                                    tACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGc                                                                           <  1:284721/68‑1 (MQ=255)

                                                                                                    tACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGc                                                                           >  2:284721/1‑68 (MQ=255)

                                                                                                    tACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATc                                        <  1:646274/103‑1 (MQ=255)

                                                                                                    tACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATc                                        >  2:646274/1‑103 (MQ=255)

                                                                                                    tACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATc                                        <  1:1729650/103‑1 (MQ=255)

                                                                                                    tACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATc                                        >  2:1729650/1‑103 (MQ=255)

                                                                                                    tACGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgt    >  1:879951/1‑139 (MQ=255)

                                                                                                      cGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaa  <  1:694719/139‑1 (MQ=255)

                                                                                                      cGGATTTTATGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaa  <  2:528578/139‑1 (MQ=255)

                                                                                                              aTGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                           <  1:1678240/42‑1 (MQ=255)

                                                                                                              aTGACCGCGCG‑GGTTATCACTAATCAAAAATGGAAATGCCCg                                                                                           >  2:1678240/1‑42 (MQ=255)

                                                                                                                      cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgt    >  2:476130/5‑122 (MQ=255)

                                                                                                                      cgcgggTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgt    <  1:476130/118‑1 (MQ=255)

                                                                                                                                 aCTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                             <  1:713635/70‑1 (MQ=255)

                                                                                                                                 aCTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                             >  2:713635/1‑70 (MQ=255)

TTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAA  >  NC_000913/3134690‑3134931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A