Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I2 R1 758 59.3 2864096 93.9% 2689386 105.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,804,068 T→C I24V (ATT→GTT)  waaB ← lipopolysaccharide 1,6‑galactosyltransferase, UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,804,0680TC100.0% 226.5 / NA 62I24V (ATT→GTT) waaBlipopolysaccharide 1,6‑galactosyltransferase, UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/31);  total (31/31)

ACGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCTATAAAATCAGA  >  NC_000913/3803934‑3804201
                                                                                                                                      |                                                                                                                                     
aCGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAacaaca                                                                                                                                   >  2:1101333/1‑139 (MQ=255)
aCGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAacaaca                                                                                                                                   >  1:751699/1‑139 (MQ=255)
        aaCCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGAt                                                                                                                           <  2:63224/139‑1 (MQ=255)
         aCCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATg                                                                                                                          <  1:1101333/139‑1 (MQ=255)
         aCCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCA‑CCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGa                                                                                                                         <  2:463139/139‑1 (MQ=255)
                    tatTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCAt                                                                                                               >  2:812534/1‑139 (MQ=255)
                                             aaTACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCg                                                                                      <  2:1050253/139‑1 (MQ=255)
                                                aCCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCa                                                                                                                                >  1:437858/1‑94 (MQ=255)
                                                aCCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCa                                                                                                                                <  2:437858/94‑1 (MQ=255)
                                                aCCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCg                                                                                   >  1:76910/1‑139 (MQ=255)
                                                  cTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGAt                                                                                 >  2:304432/1‑139 (MQ=255)
                                                    tCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATaa                                                                               >  1:343897/1‑139 (MQ=255)
                                                      cAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCAt                                                                                                               <  1:585851/105‑1 (MQ=255)
                                                      cAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCAt                                                                                                               >  2:585851/1‑105 (MQ=255)
                                                      cAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAAc                                                                             <  2:578943/139‑1 (MQ=255)
                                                       aGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACg                                                                            <  1:812534/139‑1 (MQ=255)
                                                        gCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGc                                                                           <  2:343897/139‑1 (MQ=255)
                                                        gCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGc                                                                           >  1:701140/1‑139 (MQ=255)
                                                         ccATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCtt                                                                                        <  1:236136/125‑1 (MQ=255)
                                                         ccATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCtt                                                                                        >  2:236136/1‑125 (MQ=255)
                                                         ccATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCt                                                                          >  2:98519/1‑139 (MQ=255)
                                                                  aTCCATTTTGTCATTACGACAAAAGAAAAACATTTCGCTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCACCGATAAACGCTATTTTCATa                                                                 >  1:839785/1‑139 (MQ=255)
                                                                  aTCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATa                                                                 <  2:1342162/139‑1 (MQ=255)
                                                                   tCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAg                                                                >  2:1354388/1‑139 (MQ=255)
                                                                    ccATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGa                                                               <  1:1354388/139‑1 (MQ=255)
                                                                      aTTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGaaa                                                             <  1:34386/139‑1 (MQ=255)
                                                                      aTTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGaaa                                                             <  2:751699/139‑1 (MQ=255)
                                                                       ttttGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAAc                                                            >  2:1210525/1‑139 (MQ=255)
                                                                        tttGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCa                                                                                                        >  2:476305/1‑94 (MQ=255)
                                                                        tttGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCa                                                                                                        <  1:476305/94‑1 (MQ=255)
                                                                        tttGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCTCCGATAAACGCTATTTTCATAGAAAct                                                           >  1:1131779/1‑139 (MQ=255)
                                                                          tGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGa                                                                                                                            >  1:1026013/1‑72 (MQ=255)
                                                                          tGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGa                                                                                                                            <  2:1026013/72‑1 (MQ=255)
                                                                             cATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCAt                                                      >  1:263538/1‑139 (MQ=255)
                                                                              aTTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGaaa                                                             <  2:1092316/131‑1 (MQ=255)
                                                                              aTTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGaaa                                                             >  1:1092316/1‑131 (MQ=255)
                                                                              aTTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCAtt                                                     >  1:749532/1‑139 (MQ=255)
                                                                              aTTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCAtt                                                     >  1:531827/1‑139 (MQ=255)
                                                                                    aCAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGa                                               >  2:671/1‑139 (MQ=255)
                                                                                      aaaaGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGt                                             <  2:1288083/139‑1 (MQ=255)
                                                                                             aaaCATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTc                                      <  1:304432/139‑1 (MQ=255)
                                                                                               aCATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCAc                                    <  2:749532/139‑1 (MQ=255)
                                                                                               aCATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCAc                                    <  1:672435/139‑1 (MQ=255)
                                                                                                  tttCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTaa                                                   >  2:10956/1‑121 (MQ=255)
                                                                                                  tttCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTaa                                                   <  1:10956/121‑1 (MQ=255)
                                                                                                      gcTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATCGAGTAAAACTCACTGTTTTa                             <  2:839785/138‑1 (MQ=255)
                                                                                                       cTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTaa                            <  2:531827/139‑1 (MQ=255)
                                                                                                                 gttgttGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCGGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTAtt                  >  2:486607/1‑139 (MQ=255)
                                                                                                                  ttgttgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTAttt                 <  2:263538/139‑1 (MQ=255)
                                                                                                                   tgttgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTg                <  2:701140/139‑1 (MQ=255)
                                                                                                                      tgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCa                                                                                                        >  1:720872/1‑48 (MQ=255)
                                                                                                                      tgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCa                                                                                                        <  2:720872/48‑1 (MQ=255)
                                                                                                                      tgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAAc                           >  1:1009448/1‑125 (MQ=255)
                                                                                                                      tgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCt             >  1:432813/1‑139 (MQ=255)
                                                                                                                      tgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATCTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAAc                           <  2:1009448/125‑1 (MQ=255)
                                                                                                                      tgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCACCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGt               >  1:1324407/1‑137 (MQ=255)
                                                                                                                      tgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCACCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGt               <  2:1324407/137‑1 (MQ=255)
                                                                                                                       gCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTa                             <  1:39733/122‑1 (MQ=255)
                                                                                                                       gCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTa                             >  2:39733/1‑122 (MQ=255)
                                                                                                                            aaGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCAc                                    >  1:877919/1‑110 (MQ=255)
                                                                                                                            aaGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCAc                                    <  2:877919/110‑1 (MQ=255)
                                                                                                                            aaGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCTATaaaa       >  1:742818/1‑139 (MQ=255)
                                                                                                                                 agtaACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCTATAAAATCAGa  >  2:685068/1‑139 (MQ=255)
                                                                                                                                 agtaACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCTATAAAATCAGa  >  2:146423/1‑139 (MQ=255)
                                                                                                                                   taacaaCATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTAtt                                                                       <  1:1303556/68‑1 (MQ=255)
                                                                                                                                   taacaaCATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTAtt                                                                       >  2:1303556/1‑68 (MQ=255)
                                                                                                                                      |                                                                                                                                     
ACGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCTATAAAATCAGA  >  NC_000913/3803934‑3804201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A