Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	750	51.0	2595496	93.6%	2429384	106.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,804,068	T→C	I24V (ATT→GTT)	waaB ←	lipopolysaccharide 1,6‑galactosyltransferase, UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,804,068	0	T	C	97.3%	264.6 / ‑0.7	75	I24V (ATT→GTT)	waaB	lipopolysaccharide 1,6‑galactosyltransferase, UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (42/31); minor base A (1/1); total (43/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.48e-01

CGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCTATAAAATCAGAAA  >  NC_000913/3803935‑3804203

cGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACAt                                                                                                                                    >  1:1218994/1‑139 (MQ=255)

        aCCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATg                                                                                                                            <  2:599047/139‑1 (MQ=255)

        aCCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATg                                                                                                                            <  1:271643/139‑1 (MQ=255)

             aaCGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGt                                                                                                                       >  1:1069824/1‑139 (MQ=255)

                   tatTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCAt                                                                                                                 >  2:735076/1‑139 (MQ=255)

                   tatTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCAt                                                                                                                 >  1:713257/1‑139 (MQ=255)

                            aTGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCaaa                                                                                                        <  1:735076/139‑1 (MQ=255)

                                     aGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAAt                                                                                                       >  2:922022/1‑131 (MQ=255)

                                     aGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAAt                                                                                                       <  1:922022/131‑1 (MQ=255)

                                         tttAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACg                                                                                                                                >  1:1161329/1‑102 (MQ=255)

                                         tttAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACg                                                                                                                                <  2:1161329/102‑1 (MQ=255)

                                                 cTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGAt                                                                                   >  2:993476/1‑139 (MQ=255)

                                                   tCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATaa                                                                                 >  1:333538/1‑139 (MQ=255)

                                                   tCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATaa                                                                                 >  2:355823/1‑139 (MQ=255)

                                                   tCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATaa                                                                                 >  2:453736/1‑139 (MQ=255)

                                                   tCCAGCCAACCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATaa                                                                                 >  1:842365/1‑139 (MQ=255)

                                                    ccAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAAAAACATCACGGATGa                                                                                                                           >  2:448668/1‑96 (MQ=255)

                                                    ccAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAAAAACATCACGGATGa                                                                                                                           <  1:448668/96‑1 (MQ=255)

                                                     cAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAAc                                                                               <  1:659746/139‑1 (MQ=255)

                                                           tCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCa                                                                                                          >  2:613167/1‑106 (MQ=255)

                                                           tCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCa                                                                                                          <  1:613167/106‑1 (MQ=255)

                                                           tCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAg                                                                                             <  1:332507/119‑1 (MQ=255)

                                                           tCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAg                                                                                             >  2:332507/1‑119 (MQ=255)

                                                            cccTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACg                                                                                                                                <  2:604577/83‑1 (MQ=255)

                                                            cccTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACg                                                                                                                                >  1:604577/1‑83 (MQ=255)

                                                            cccTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTAttt                                                                        >  2:815656/1‑139 (MQ=255)

                                                             ccTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCa                                                                                                          >  1:438084/1‑104 (MQ=255)

                                                             ccTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCa                                                                                                          <  2:438084/104‑1 (MQ=255)

                                                                  tCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAg                                                                  <  1:815656/139‑1 (MQ=255)

                                                                     aTTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGaaa                                                               <  2:1218994/139‑1 (MQ=255)

                                                                      ttttGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAAc                                                              >  1:127890/1‑139 (MQ=255)

                                                                       tttGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATa                                                                                  >  2:303548/1‑118 (MQ=255)

                                                                       tttGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATa                                                                                  <  1:303548/118‑1 (MQ=255)

                                                                       tttGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGaaa                                                               >  1:540947/1‑137 (MQ=255)

                                                                       tttGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGaaa                                                               <  2:540947/137‑1 (MQ=255)

                                                                       tttGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAAct                                                             >  1:619301/1‑139 (MQ=255)

                                                                         tGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGaaa                                                               >  2:621404/1‑135 (MQ=255)

                                                                         tGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGaaa                                                               <  1:621404/135‑1 (MQ=255)

                                                                            cATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAAct                                                             >  1:596321/1‑134 (MQ=255)

                                                                            cATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAAct                                                             <  2:596321/134‑1 (MQ=255)

                                                                            cATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCAt                                                        >  2:243335/1‑139 (MQ=255)

                                                                             aTTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCAtt                                                       >  1:1290449/1‑139 (MQ=255)

                                                                                aCGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACtctc                                                          <  1:132631/133‑1 (MQ=255)

                                                                                aCGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACtctc                                                          >  2:132631/1‑133 (MQ=255)

                                                                                   aCAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGa                                                 <  2:1069824/139‑1 (MQ=255)

                                                                                       aaGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTaa                                             >  2:679157/1‑139 (MQ=255)

                                                                                              aCATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCAc                                      <  2:417455/139‑1 (MQ=255)

                                                                                                 tttCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGt                                   >  1:1076168/1‑139 (MQ=255)

                                                                                                  ttCACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGtt                                  >  2:405046/1‑139 (MQ=255)

                                                                                                    cACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCa                                       >  1:973388/1‑132 (MQ=255)

                                                                                                    cACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCa                                       <  2:973388/132‑1 (MQ=255)

                                                                                                    cACTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGtttt                                >  1:684669/1‑139 (MQ=255)

                                                                                                     aCTTTGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTa                               <  2:713257/139‑1 (MQ=255)

                                                                                                       tttGGATATGTTGTTGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAAc                             <  1:355823/139‑1 (MQ=255)

                                                                                                                gttgttGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCt                                                                            >  1:690265/1‑83 (MQ=255)

                                                                                                                gttgttGCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCt                                                                            <  2:690265/83‑1 (MQ=255)

                                                                                                                  tgttgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTg                  <  2:1290449/139‑1 (MQ=255)

                                                                                                                   gttgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGt                 >  1:1296207/1‑139 (MQ=255)

                                                                                                                     tgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTaa                              >  2:34564/1‑124 (MQ=255)

                                                                                                                     tgCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTaa                              <  1:34564/124‑1 (MQ=255)

                                                                                                                      gCCTGAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCta              >  1:559317/1‑139 (MQ=255)

                                                                                                                         tGAAGGTAGTAACAACATCACGGATGACAGTTTCCAt                                                                                                                 <  1:1001370/37‑1 (MQ=255)

                                                                                                                         tGAAGGTAGTAACAACATCACGGATGACAGTTTCCAt                                                                                                                 >  2:1001370/1‑37 (MQ=255)

                                                                                                                          gAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCg                                                                                        >  2:134964/1‑61 (MQ=255)

                                                                                                                          gAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCg                                                                                        <  1:134964/61‑1 (MQ=255)

                                                                                                                          gAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTaa                              >  2:237602/1‑119 (MQ=255)

                                                                                                                          gAAGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTaa                              <  1:237602/119‑1 (MQ=255)

                                                                                                                           aaGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCt                                                                                           <  2:483323/57‑1 (MQ=255)

                                                                                                                           aaGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCt                                                                                           >  1:483323/1‑57 (MQ=255)

                                                                                                                           aaGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGaaa                                                               <  1:651938/85‑1 (MQ=255)

                                                                                                                           aaGGTAGTAACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGaaa                                                               >  2:651938/1‑85 (MQ=255)

                                                                                                                                agtaACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATa                                                                   >  1:989870/1‑76 (MQ=255)

                                                                                                                                agtaACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATa                                                                   <  2:989870/76‑1 (MQ=255)

                                                                                                                                agtaACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCTATAAAATCAGa    >  1:661305/1‑139 (MQ=255)

                                                                                                                                agtaACAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCTATAAAATCAGa    >  1:255725/1‑139 (MQ=255)

                                                                                                                                  taacaaCATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCTATAAAATCAGaaa  <  2:661305/139‑1 (MQ=255)

CGCAAAAAACCTAAACGAATATTGGAAAATGAACATGAGTATTTAATACCTTCCAGCCATCCCTTATCCATTTTGTCATTACGACAAAAGAAAAACATTTCACTTTGGATATGTTGTTGCCTGAAGGTAGTAATAACATCACGGATGACAGTTTCCATGCCGCCAAATCCAGAAACAGCTTCGCCGATAAACGCTATTTTCATAGAAACTCTCATTAATTGAGTAAAACTCACTGTTTTAACGGTTTTATTTGTCTATAAAATCAGAAA  >  NC_000913/3803935‑3804203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A