Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,012,553 |
Δ1 bp |
intergenic (+160/+61) |
yqeA → / ← yqeB |
putative amino acid kinase/XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,012,552 | 0 | G | . | 100.0%
| 129.8
/ NA
| 31 | intergenic (+159/+62) | yqeA/yqeB | putative amino acid kinase/XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (14/17); total (14/17) |
TCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTACG > NC_000913/3012419‑3012686
|
tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAAtt > 1:449019/1‑139 (MQ=255)
ccGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAAttt > 2:815870/1‑139 (MQ=255)
tGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGtcat < 1:105537/139‑1 (MQ=255)
gtGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAgg < 2:449019/139‑1 (MQ=255)
gtGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAgg < 1:815870/139‑1 (MQ=255)
gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg < 2:476394/139‑1 (MQ=255)
gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg < 2:723375/139‑1 (MQ=255)
gcTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg < 1:658367/139‑1 (MQ=255)
cTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg > 1:690791/1‑98 (MQ=255)
cTACAAAAAATCGCAAATTCACTAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCCATTTGGCGTTTGTCATCAGTCTCAGGCCgg < 2:690791/98‑1 (MQ=25)
cATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAgg < 1:566197/86‑1 (MQ=255)
cATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAgg > 2:566197/1‑86 (MQ=255)
cATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCActtc > 1:259390/1‑139 (MQ=255)
aTCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCActtct > 1:56025/1‑139 (MQ=255)
cGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTc < 2:207444/127‑1 (MQ=255)
cGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTc > 1:207444/1‑127 (MQ=255)
tCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGc < 1:584115/99‑1 (MQ=255)
tCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATACGGc > 2:584115/1‑99 (MQ=255)
aaTAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCttt < 2:243838/139‑1 (MQ=255)
tAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCa < 1:464607/139‑1 (MQ=255)
cACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAg > 1:30447/1‑59 (MQ=255)
cACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAg < 2:30447/59‑1 (MQ=255)
cACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCa < 2:823445/127‑1 (MQ=255)
cACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCa > 1:823445/1‑127 (MQ=255)
cACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCa > 1:573662/1‑139 (MQ=255)
gATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa < 1:665151/139‑1 (MQ=255)
tAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACg > 2:88902/1‑139 (MQ=255)
tAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgggtggg < 2:134035/48‑1 (MQ=21)
tAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgggtggg > 1:134035/1‑48 (MQ=25)
cgcATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTa > 2:227441/1‑139 (MQ=255)
cATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTAcg < 1:88902/139‑1 (MQ=255)
|
TCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTACG > NC_000913/3012419‑3012686
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
