Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	760	52.7	2744488	90.2%	2475528	105.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,012,553	Δ1 bp	intergenic (+160/+61)	yqeA → / ← yqeB	putative amino acid kinase/XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,012,552	0	G	.	100.0%	216.4 / NA	52	intergenic (+159/+62)	yqeA/yqeB	putative amino acid kinase/XdhC‑CoxI family protein with NAD(P)‑binding Rossman fold
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (26/26); total (26/26)

TCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGA‑‑GCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTACG  >  NC_000913/3012419‑3012686

tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGA‑‑GCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAAtt                                                                                                                                  >  1:57598/1‑139 (MQ=255)

tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGA‑‑GCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAAtt                                                                                                                                  >  2:466975/1‑139 (MQ=255)

tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGA‑‑GCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAAtt                                                                                                                                  >  2:322921/1‑139 (MQ=255)

tCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGA‑‑GCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAAtt                                                                                                                                  >  2:222735/1‑139 (MQ=255)

     ccAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTgg                                                                                                                               >  2:441164/1‑139 (MQ=37)

             tGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGA‑‑GCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGtcat                                                                                                                     <  1:1019662/139‑1 (MQ=255)

                         gcgcTTTGTTGATGCCGGATGCGGCGCGA‑‑GCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGcc                                                                                                         >  1:193887/1‑139 (MQ=255)

                         gcgcTTTGTTGATGCCGGATGCGGCGCGA‑‑GCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGcc                                                                                                         >  2:541746/1‑139 (MQ=255)

                           gcTTTGTTGATGCCGGATGCGGCGCGA‑‑GCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  2:57598/139‑1 (MQ=255)

                           gcTTTGTTGATGCCGGATGCGGCGCGA‑‑GCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  1:541746/139‑1 (MQ=255)

                           gcTTTGTTGATGCCGGATGCGGCGCGA‑‑GCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  1:825225/139‑1 (MQ=255)

                           gcTTTGTTGATGCCGGATGCGGCGCGA‑‑GCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  2:490800/139‑1 (MQ=255)

                                           aTGCGGCGCGA‑‑GCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGtcat                                                                                                                     <  1:221333/109‑1 (MQ=255)

                                           aTGCGGCGCGA‑‑GCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGtcat                                                                                                                     >  2:221333/1‑109 (MQ=255)

                                              cggcgCGA‑‑GCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGc                                                                                    <  1:466975/139‑1 (MQ=255)

                                                   cgA‑‑GCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGAGCATCA‑GCAATTTGGCGTTTGTCATCACTCTCAGGCCGGGTGGGTAATACCATCCGGCCAttt                                                                               >  2:823936/1‑139 (MQ=255)

                                                               aTCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGc                                                                     <  1:441164/139‑1 (MQ=255)

                                                                      cTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       >  1:572288/1‑98 (MQ=255)

                                                                      cTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  2:572288/98‑1 (MQ=255)

                                                                      cTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTcc                                                              >  2:705586/1‑139 (MQ=255)

                                                                          aaaaCATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCa                                                          <  1:1361573/139‑1 (MQ=255)

                                                                            aaCATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCAct                                                        <  1:1217934/139‑1 (MQ=255)

                                                                                  gCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCtttt                                                  <  2:193887/139‑1 (MQ=255)

                                                                                   cAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGtctc                                                                                                              <  1:573991/78‑1 (MQ=255)

                                                                                   cAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGtctc                                                                                                              >  2:573991/1‑78 (MQ=255)

                                                                                   cAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  1:761832/85‑1 (MQ=255)

                                                                                   cAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       >  2:761832/1‑85 (MQ=255)

                                                                                    aaaTTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGt                                                >  2:695825/1‑139 (MQ=255)

                                                                                            tAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCa                                        <  1:222735/139‑1 (MQ=255)

                                                                                                 tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       >  1:788128/1‑71 (MQ=255)

                                                                                                 tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCgg                                                                                                       <  2:788128/71‑1 (MQ=255)

                                                                                                 tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCttt                                          >  1:305266/1‑132 (MQ=255)

                                                                                                 tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCttt                                          <  2:305266/132‑1 (MQ=255)

                                                                                                 tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGccc                                   >  2:1152205/1‑139 (MQ=255)

                                                                                                 tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGccc                                   >  1:538136/1‑139 (MQ=255)

                                                                                                 tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGccc                                   >  1:660455/1‑139 (MQ=255)

                                                                                                 tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGccc                                   >  2:387645/1‑139 (MQ=255)

                                                                                                 tGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGccc                                   >  2:1161596/1‑139 (MQ=255)

                                                                                                     aTGCACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGAt                               <  2:660455/139‑1 (MQ=255)

                                                                                                        cACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGt                                                                                                                 >  2:776733/1‑54 (MQ=255)

                                                                                                        cACTTGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGt                                                                                                                 <  1:776733/54‑1 (MQ=255)

                                                                                                           ttGTAGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCa                         <  1:823936/139‑1 (MQ=255)

                                                                                                               aGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAAcat                     <  1:705586/139‑1 (MQ=255)

                                                                                                               aGGCCTGATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAAcat                     <  2:538136/139‑1 (MQ=255)

                                                                                                                     gATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCgg                                                                                     >  2:400839/1‑69 (MQ=255)

                                                                                                                     gATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCgg                                                                                     <  1:400839/69‑1 (MQ=255)

                                                                                                                     gATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa               >  1:1030740/1‑139 (MQ=255)

                                                                                                                     gATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa               >  2:606759/1‑139 (MQ=255)

                                                                                                                     gATAAGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTaa               <  1:587914/139‑1 (MQ=255)

                                                                                                                         aGCGTAGCGCATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGcc           <  1:606759/139‑1 (MQ=255)

                                                                                                                                cgcATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTa    >  1:132446/1‑139 (MQ=255)

                                                                                                                                  cATCA‑GCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTAcg  <  2:1030740/139‑1 (MQ=255)

TCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGA‑‑GCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTGGCGTTTGTCATCAGTCTCAGGCCGGGTGGGTAATACCATCCGGCCATTTTCATTTAAGCCACTTCCAGCACTTCTTTTGTCGCTTTCACGCCCTGATGCATCAACATCATTAACGCCTCAAGTACG  >  NC_000913/3012419‑3012686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A