Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,297,157 |
T→C |
T328A (ACA→GCA) |
yraQ ← |
putative inner membrane permease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,297,157 | 0 | T | C | 100.0%
| 91.6
/ NA
| 27 | T328A (ACA→GCA) | yraQ | putative inner membrane permease |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/12); total (15/12) |
TCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGTCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATC > NC_000913/3297026‑3297290
|
tCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCACCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCAt > 1:976325/1‑139 (MQ=255)
aTACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCgg > 2:87605/1‑139 (MQ=255)
aGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCgcagc > 1:138943/1‑139 (MQ=255)
aTCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACt > 1:744362/1‑139 (MQ=255)
tttCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGa < 1:1098798/139‑1 (MQ=255)
ttCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAg > 2:892698/1‑139 (MQ=255)
ttCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAg > 1:372990/1‑139 (MQ=255)
cTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGa > 1:538542/1‑89 (MQ=255)
cTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGa < 2:538542/89‑1 (MQ=255)
aaTCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGtcatca > 1:326432/1‑139 (MQ=255)
aTCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCa < 1:1083000/114‑1 (MQ=255)
aTCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCa > 2:1083000/1‑114 (MQ=255)
gCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGc < 2:1112875/139‑1 (MQ=255)
cccGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGATGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCg > 1:147707/1‑139 (MQ=255)
cccGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCg < 2:744362/139‑1 (MQ=255)
cacCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGc < 1:813139/139‑1 (MQ=255)
ccAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCa < 2:326432/139‑1 (MQ=255)
cacTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGcatc < 2:372990/139‑1 (MQ=255)
cacTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGcatc > 1:552868/1‑139 (MQ=255)
caTCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAg < 1:688394/99‑1 (MQ=255)
caTCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAg > 2:688394/1‑99 (MQ=255)
caTCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGc < 2:521368/108‑1 (MQ=255)
caTCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGc > 1:521368/1‑108 (MQ=255)
tCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCgg > 1:866145/1‑76 (MQ=255)
tCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCgg < 2:866145/76‑1 (MQ=255)
tCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTAc > 1:257408/1‑139 (MQ=255)
cccTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGATGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATc < 2:147707/139‑1 (MQ=255)
|
TCAGGCCTACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGTCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTACAATC > NC_000913/3297026‑3297290
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
