Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	773	79.9	4112934	92.2%	3792125	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,297,157	T→C	T328A (ACA→GCA)	yraQ ←	putative inner membrane permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,297,157	0	T	C	100.0%	138.2 / NA	39	T328A (ACA→GCA)	yraQ	putative inner membrane permease
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/20); total (19/20)

TACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGTCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTAC  >  NC_000913/3297033‑3297286

tACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCtt                                                                                                                     <  2:713696/139‑1 (MQ=255)

  cAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTc                                                                                                                   >  1:1289290/1‑139 (MQ=255)

                 tAGGTCAGATAAGGCGTTTACGCCGCATCCGACATG‑TTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTgc                                                                                                   <  2:142401/139‑1 (MQ=255)

                   ggTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTgcg                                                                                                  <  1:1957741/139‑1 (MQ=255)

                                      gccgcATCCGACATGTTTTCCCTCAAATCAGAACAACCGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGc                                                                               <  1:1312697/139‑1 (MQ=255)

                                        cgcATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCaa                                                                             >  2:34848/1‑139 (MQ=255)

                                           aTCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGc                                                                                                                 >  1:975753/1‑100 (MQ=255)

                                           aTCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGc                                                                                                                 <  2:975753/100‑1 (MQ=255)

                                                aCATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTg                                                                     >  1:1679047/1‑139 (MQ=255)

                                                    gTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATa                                                                                                                           <  1:1008141/81‑1 (MQ=255)

                                                    gTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATa                                                                                                                           >  2:1008141/1‑81 (MQ=255)

                                                      tttCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGa                                                               <  1:34848/139‑1 (MQ=255)

                                                      tttCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGa                                                               <  2:1289290/139‑1 (MQ=255)

                                                      tttCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGa                                                               <  1:666620/139‑1 (MQ=255)

                                                      tttCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGa                                                               <  1:972994/139‑1 (MQ=255)

                                                       ttCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCgcagc                                                                                              >  1:559320/1‑107 (MQ=255)

                                                       ttCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCgcagc                                                                                              <  2:559320/107‑1 (MQ=255)

                                                                    gAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGa                                                               >  2:322570/1‑125 (MQ=255)

                                                                    gAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGa                                                               <  1:322570/125‑1 (MQ=255)

                                                                     aacaacAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATg                                                >  1:1990217/1‑139 (MQ=255)

                                                                     aacaacAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACACTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATg                                                >  1:1216268/1‑139 (MQ=255)

                                                                          caGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAgc                                           >  1:979034/1‑139 (MQ=255)

                                                                          caGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAgc                                           >  1:1624525/1‑139 (MQ=255)

                                                                               ccAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTgcgc                                                                                                 >  1:1293962/1‑80 (MQ=255)

                                                                               ccAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTgcgc                                                                                                 <  2:1293962/80‑1 (MQ=255)

                                                                                  gCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATATGGCTTTCGCCGGGa                                                                                                            <  2:1249229/66‑1 (MQ=255)

                                                                                  gCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGa                                                                                                            >  1:1249229/1‑66 (MQ=255)

                                                                                  gCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGc                                                             >  1:752269/1‑113 (MQ=255)

                                                                                  gCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGc                                                             <  2:752269/113‑1 (MQ=255)

                                                                                   cccGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCg                                  <  2:1624525/139‑1 (MQ=255)

                                                                                   cccGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCg                                  <  2:168475/139‑1 (MQ=255)

                                                                                                 cacCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGc                    >  1:1257973/1‑139 (MQ=255)

                                                                                                   ccAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCa                  <  1:572462/139‑1 (MQ=255)

                                                                                                        cacTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCa                           >  1:189865/1‑125 (MQ=255)

                                                                                                        cacTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCa                           <  2:189865/125‑1 (MQ=255)

                                                                                                         acTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGCTCCCATACCTGCCAGcatca            >  1:902897/1‑139 (MQ=255)

                                                                                                          cTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGcatcat           <  2:1216268/139‑1 (MQ=255)

                                                                                                           tGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCatcatc          >  1:1412122/1‑139 (MQ=255)

                                                                                                                   tCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTAc  >  1:567406/1‑139 (MQ=255)

TACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGTCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTAC  >  NC_000913/3297033‑3297286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A