Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F1 I2 R1
|
760 |
67.6 |
3323148 |
92.7% |
3080558 |
109.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,051,321 |
G→A |
R333C (CGT→TGT) |
ubiI ← |
2‑octaprenylphenol hydroxylase, FAD‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,051,321 | 0 | G | A | 100.0%
| 130.8
/ NA
| 41 | R333C (CGT→TGT) | ubiI | 2‑octaprenylphenol hydroxylase, FAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/22); total (19/22) |
AGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCG > NC_000913/3051185‑3051451
|
aGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGAcaca < 1:523257/139‑1 (MQ=255)
tgtCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATa < 1:805721/139‑1 (MQ=255)
gtCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATaa < 2:1545023/139‑1 (MQ=255)
gCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGt < 2:779512/139‑1 (MQ=255)
cagcagTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGa < 1:1250141/139‑1 (MQ=255)
agcagTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGAc > 1:620183/1‑139 (MQ=255)
cagTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGa > 1:506438/1‑139 (MQ=255)
agTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGAt < 1:1086926/139‑1 (MQ=255)
cGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAAt < 2:1354133/139‑1 (MQ=255)
cGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAAt < 1:1510165/139‑1 (MQ=255)
cGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAAt < 2:620183/139‑1 (MQ=255)
gAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCa > 1:1251429/1‑139 (MQ=255)
gAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCa > 1:256113/1‑139 (MQ=255)
cAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGctct < 1:1036635/139‑1 (MQ=255)
aTCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTgca > 1:1554954/1‑139 (MQ=255)
cgcgGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTgcagc > 2:14463/1‑139 (MQ=255)
gCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGcc < 1:456540/139‑1 (MQ=255)
tACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAAt > 1:321507/1‑112 (MQ=255)
tACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAAt < 2:321507/112‑1 (MQ=255)
tACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCgag > 1:1622547/1‑139 (MQ=255)
cagccagcATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGct < 2:1378501/114‑1 (MQ=255)
cagccagcATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGct > 1:1378501/1‑114 (MQ=255)
agccagcATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGAttt > 1:934201/1‑139 (MQ=255)
agcATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTAccc > 2:1660839/1‑139 (MQ=255)
cATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCtt < 2:1449731/68‑1 (MQ=255)
cATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCtt > 1:1008269/1‑68 (MQ=255)
cATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCtt < 2:1008269/68‑1 (MQ=255)
cATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCtt > 1:1449731/1‑68 (MQ=255)
cATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTAccccc > 2:577747/1‑139 (MQ=255)
cACTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAg < 1:352323/139‑1 (MQ=255)
cTGTGCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCg < 2:1251429/139‑1 (MQ=255)
tgCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGt > 1:1604257/1‑139 (MQ=255)
tgCTTGCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGt > 2:1480292/1‑139 (MQ=255)
gCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGc < 2:972801/61‑1 (MQ=255)
gCGGCTACGCTCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGc > 1:972801/1‑61 (MQ=255)
tCATAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTgcgg < 1:14463/139‑1 (MQ=255)
aTAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGAt > 1:804527/1‑98 (MQ=255)
aTAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGAt < 2:804527/98‑1 (MQ=255)
aTAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTgcggcg < 2:1309825/139‑1 (MQ=255)
aTAGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTgcggcg < 1:1660839/139‑1 (MQ=255)
aGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGtt > 2:1101265/1‑60 (MQ=255)
aGCGACACAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGtt < 1:1101265/60‑1 (MQ=255)
|
AGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTGGTACCGGAAAACAGATCGCGGAATCCCTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCG > NC_000913/3051185‑3051451
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
