Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,766,316	G→A	1378 (TAG→TAA)	rhsA →	Rhs protein with putative toxin 55 domain, putative polysaccharide synthesis/export protein, putative neighboring cell growth inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,766,316	0	G	A	99.1%	353.0 / ‑4.1	109	coding (4134/4134 nt)	rhsA	Rhs protein with putative toxin 55 domain, putative polysaccharide synthesis/export protein, putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (47/61); minor base . (0/1); total (47/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

Only 100 of 109 total aligned reads displayed.

ATGGGAAAAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAATTATTTCGCTTACTTGATGATCACAATTCC  >  NC_000913/3766180‑3766447

aTGGGAAAAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAAcc                                                                                                                                    >  1:90020/1‑139 (MQ=255)

 tGGGAAAAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAAccc                                                                                                                                   >  2:806774/1‑139 (MQ=255)

      aaaaaCCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTtgt‑g                                                                                                                             <  1:451396/139‑1 (MQ=255)

       aaaaCCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑gg                                                                                                                            <  2:1352298/139‑1 (MQ=255)

               aCAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCt                                                                                                                    >  2:96217/1‑139 (MQ=255)

                 aGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAG‑TGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCa                                                                                                                 <  1:1000613/139‑1 (MQ=255)

                  gATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCa                                                                                                                 <  2:1616228/139‑1 (MQ=255)

                  gATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCa                                                                                                                 <  2:1112924/139‑1 (MQ=255)

                   aTGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCaa                                                                                                                >  2:1076148/1‑139 (MQ=255)

                    tgtgACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAAt                                                                                                               <  1:825935/139‑1 (MQ=255)

                     gtgACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGTCAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCa                                                                                                                 >  1:710136/1‑136 (MQ=255)

                      tgACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGt                                                                                                             >  2:829542/1‑139 (MQ=255)

                       gACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCATACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTc                                                                                                            <  1:1080502/139‑1 (MQ=255)

                       gACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTc                                                                                                            <  2:287190/139‑1 (MQ=255)

                       gACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTc                                                                                                            <  2:466279/139‑1 (MQ=255)

                             cTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAGTTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAatac                                                                                                      >  1:1031307/1‑139 (MQ=255)

                             cTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCa                                                                                                                 <  2:571160/128‑1 (MQ=255)

                             cTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCa                                                                                                                 <  2:653280/128‑1 (MQ=255)

                             cTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCa                                                                                                                 >  1:653280/1‑128 (MQ=255)

                             cTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCa                                                                                                                 >  1:571160/1‑128 (MQ=255)

                             cTTCAGGAACTAATTGATTGGGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAACGTCAAatcc                                                                                                      >  2:974979/1‑137 (MQ=255)

                               tCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAatacat                                                                                                    >  2:571196/1‑139 (MQ=255)

                                cAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAatacata                                                                                                   >  1:476357/1‑139 (MQ=255)

                                     aCTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCaga                                                                                              >  2:1247210/1‑139 (MQ=255)

                                      cTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCa                                                                                                                 <  2:1453657/119‑1 (MQ=255)

                                      cTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCa                                                                                                                 >  1:1453657/1‑119 (MQ=255)

                                         attgattgTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCagaaaag                                                                                          >  1:1507241/1‑139 (MQ=255)

                                            gattgTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCagaaaagaaa                                                                                       <  1:258888/139‑1 (MQ=255)

                                            gattgTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCagaaaagaaa                                                                                       <  2:1507241/139‑1 (MQ=255)

                                               tgtgGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGc                                                                                    <  2:1511121/139‑1 (MQ=255)

                                                 tggtgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCGATGTCAAATACATACCAGAAAAGAAAGGCaa                                                                                  <  1:449214/139‑1 (MQ=255)

                                                 tggtgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGc                                                                                    <  1:837778/137‑1 (MQ=255)

                                                 tggtgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGc                                                                                    >  2:837778/1‑137 (MQ=255)

                                                 tggtgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCaa                                                                                  <  2:846989/139‑1 (MQ=255)

                                                   gtgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCa                                                                                                                 >  2:4955/1‑106 (MQ=255)

                                                   gtgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCa                                                                                                                 <  1:4955/106‑1 (MQ=255)

                                                   gtgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAataca                                                                                                     >  1:593860/1‑118 (MQ=255)

                                                   gtgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAataca                                                                                                     <  2:593860/118‑1 (MQ=255)

                                                    tgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                                               <  1:1586914/139‑1 (MQ=255)

                                                    tgATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                                               <  1:1355319/139‑1 (MQ=255)

                                                      atatTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTaaa                                                                             <  1:96217/139‑1 (MQ=255)

                                                      atatTAGTGCTAAAGATGCAAAAAGCACAAAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTaaa                                                                             <  2:1013441/139‑1 (MQ=255)

                                                          tAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                         <  2:1031307/139‑1 (MQ=255)

                                                            gTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTAt                                                                       <  2:270877/139‑1 (MQ=255)

                                                             tGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTtgt‑g                                                                                                                             <  1:3127/84‑1 (MQ=255)

                                                             tGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTtgt‑g                                                                                                                             >  2:3127/1‑84 (MQ=255)

                                                             tGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCa                                                                                                                 >  1:941606/1‑96 (MQ=255)

                                                             tGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCa                                                                                                                 <  2:941606/96‑1 (MQ=255)

                                                             tGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCagaaaagaaa                                                                                       >  2:544315/1‑122 (MQ=255)

                                                              gCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATgg                                                                     <  1:1247210/139‑1 (MQ=255)

                                                               cTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGt                                                                    <  1:196418/139‑1 (MQ=255)

                                                                  aaGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTta                                                                 >  2:1299958/1‑139 (MQ=255)

                                                                       gCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                                               >  2:301148/1‑120 (MQ=255)

                                                                       gCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                                                               <  1:301148/120‑1 (MQ=255)

                                                                       gCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                         >  1:54174/1‑126 (MQ=255)

                                                                       gCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                         <  2:54174/126‑1 (MQ=255)

                                                                       gCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTtatata                                                             >  2:296568/1‑138 (MQ=255)

                                                                       gCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTtatata                                                             <  1:296568/138‑1 (MQ=255)

                                                                       gCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATaa                                                            >  1:1445877/1‑139 (MQ=255)

                                                                       gCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATaa                                                            >  1:126758/1‑139 (MQ=255)

                                                                        cAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAAt                                                           <  2:1445877/139‑1 (MQ=255)

                                                                        cAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAAt                                                           <  2:48450/139‑1 (MQ=255)

                                                                        cAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAAt                                                           <  1:1486905/139‑1 (MQ=255)

                                                                               cacacaGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGt                                                     >  2:1275396/1‑139 (MQ=255)

                                                                                 cacaGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAg                                                  <  1:129085/139‑1 (MQ=255)

                                                                                  acaGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTtgt‑g                                                                                                                             <  2:1625942/63‑1 (MQ=255)

                                                                                  acaGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTtgt‑g                                                                                                                             >  1:1625942/1‑63 (MQ=255)

                                                                                  acaGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                         <  2:115061/115‑1 (MQ=255)

                                                                                  acaGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                         >  1:115061/1‑115 (MQ=255)

                                                                                         gCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTaaa                                          >  1:332991/1‑139 (MQ=255)

                                                                                         gCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGAAA‑CCCTTGT‑GGAGGTTCCTGCa                                                                                                                 <  1:741813/67‑1 (MQ=25)

                                                                                         gCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGA‑AACCCTTGT‑GGAGGTTCCTGCa                                                                                                                 >  2:741813/1‑67 (MQ=25)

                                                                                         gCCTGGAAATGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTAGGGTTTATATAATCAATGTAAGAAACTaaa                                          <  1:410217/139‑1 (MQ=255)

                                                                                              gAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTaaa                                                                             <  2:647433/99‑1 (MQ=255)

                                                                                              gAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTaaa                                                                             >  1:647433/1‑99 (MQ=255)

                                                                                                  tGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCagaaaaga                                                                                         >  2:1069967/1‑83 (MQ=255)

                                                                                                  tGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCagaaaaga                                                                                         <  1:1069967/83‑1 (MQ=255)

                                                                                                   gtcgtcACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCGTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                         <  2:1172209/98‑1 (MQ=255)

                                                                                                   gtcgtcACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                         >  1:1172209/1‑98 (MQ=255)

                                                                                                   gtcgtcACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTtatata                                                             <  2:1316370/110‑1 (MQ=255)

                                                                                                   gtcgtcACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTtatata                                                             >  1:1316370/1‑110 (MQ=255)

                                                                                                      gtcACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAatta                             <  2:328079/139‑1 (MQ=255)

                                                                                                         aCTCCAGACAGTCAAACGGTAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAATTAttt                          >  2:919765/1‑139 (MQ=255)

                                                                                                           tCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCa                                                                                                                 <  2:1567058/50‑1 (MQ=255)

                                                                                                           tCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCa                                                                                                                 >  1:1567058/1‑50 (MQ=255)

                                                                                                             cAGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAATTATTTCGCt                      <  2:665046/139‑1 (MQ=255)

                                                                                                              aGACAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAATTATTTCGCtt                     >  2:1391482/1‑139 (MQ=255)

                                                                                                                aCAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAATTATTTCGCTTAc                   <  1:1386681/139‑1 (MQ=255)

                                                                                                                aCAGTCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGAAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAATTATTTCGCTTAc                   <  2:332991/139‑1 (MQ=255)

                                                                                                                    tCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAATTATTTCGCTTACTtga               >  2:1114856/1‑139 (MQ=255)

                                                                                                                    tCAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAATTATTTCGCTTACTtga               >  2:887906/1‑139 (MQ=255)

                                                                                                                     cAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCa                                                                                                                 >  1:994436/1‑40 (MQ=255)

                                                                                                                     cAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCagaaaa                                                                                           >  1:264354/1‑62 (MQ=255)

                                                                                                                     cAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCagaaaa                                                                                           <  2:264354/62‑1 (MQ=255)

                                                                                                                     cAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                         <  1:761897/80‑1 (MQ=255)

                                                                                                                     cAAACGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                                                         >  2:761897/1‑80 (MQ=255)

                                                                                                                         cGATAAAAAAAGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAATTATTTCGCTTACTTGATGATc          >  1:302742/1‑139 (MQ=255)

                                                                                                                             aaaaaaaGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAATTATTTCGCTTACTTGATGATCACaa      <  1:571196/139‑1 (MQ=255)

                                                                                                                              aaaaaaGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAATTATTTCGCTTACTTGATGATCACAAt     >  1:1422922/1‑139 (MQ=255)

                                                                                                                                 aaaGATAACCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAATTATTTCGCTTACTTGATGATCACAATTcc  <  1:829542/139‑1 (MQ=255)

ATGGGAAAAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGT‑GGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGATGATGAATTATTTCGCTTACTTGATGATCACAATTCC  >  NC_000913/3766180‑3766447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A