Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	760	52.7	2744488	90.2%	2475528	105.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,766,316	G→A	1378 (TAG→TAA)	rhsA →	Rhs protein with putative toxin 55 domain, putative polysaccharide synthesis/export protein, putative neighboring cell growth inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,766,316	0	G	A	100.0%	207.7 / NA	64	1378 (TAG→TAA)	rhsA	Rhs protein with putative toxin 55 domain, putative polysaccharide synthesis/export protein, putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/36); total (28/36)

AAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGA  >  NC_000913/3766187‑3766410

aaaaCCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTgg                                                                                       <  2:832246/139‑1 (MQ=255)

      cGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTc                                                                                 >  2:1173183/1‑139 (MQ=255)

         cAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCGAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTg                                                                              <  2:1270996/139‑1 (MQ=255)

           gttgtgACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                            <  2:1049740/137‑1 (MQ=255)

           gATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                            <  1:899174/139‑1 (MQ=255)

           gATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                            <  1:1188457/139‑1 (MQ=255)

                  cGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCaa                                                                     <  1:735542/139‑1 (MQ=255)

                   gTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCaaa                                                                    >  2:274838/1‑139 (MQ=255)

                            gAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTc                                                                       >  1:1224194/1‑127 (MQ=255)

                            gAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTc                                                                       <  2:1224194/127‑1 (MQ=255)

                               cTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACAagaa                                                        >  1:368275/1‑139 (MQ=255)

                                 aaTTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                            <  2:1231645/117‑1 (MQ=255)

                                 aaTTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                            >  1:1231645/1‑117 (MQ=255)

                                 aaTTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCagaaaa                                                      >  1:1208881/1‑139 (MQ=255)

                                 aaTTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCagaaaa                                                      >  1:91675/1‑139 (MQ=255)

                                   ttgattgTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCagaaaaga                                                    <  1:1173183/139‑1 (MQ=255)

                                       ttgTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAgg                                                <  2:10609/139‑1 (MQ=255)

                                              gATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTAAAATACATACCAGAAAAGAAAGGCAAGTaa                                         >  1:1321652/1‑139 (MQ=255)

                                               atatTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTaaa                                        <  2:1208881/139‑1 (MQ=255)

                                                   taatGCTAAAGATTCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAATCCGTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                    <  1:904326/136‑1 (MQ=21)

                                                   tAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                    <  2:1101219/139‑1 (MQ=255)

                                                    aGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGa                                                                                      >  1:995666/1‑88 (MQ=255)

                                                    aGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGa                                                                                      <  2:131398/88‑1 (MQ=255)

                                                    aGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGa                                                                                      <  2:995666/88‑1 (MQ=255)

                                                    aGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGa                                                                                      >  1:131398/1‑88 (MQ=255)

                                                     gTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                    <  1:130816/137‑1 (MQ=255)

                                                     gTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                    >  2:130816/1‑137 (MQ=255)

                                                      tGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAataca                                                                <  1:897134/108‑1 (MQ=255)

                                                      tGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAataca                                                                >  2:897134/1‑108 (MQ=255)

                                                      tGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTaaa                                        >  2:1129737/1‑132 (MQ=255)

                                                      tGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTaaa                                        <  1:1129737/132‑1 (MQ=255)

                                                       gCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGa                                                                                      <  1:316937/85‑1 (MQ=255)

                                                       gCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGa                                                                                      >  2:316937/1‑85 (MQ=255)

                                                        cTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGt                               <  2:1130327/139‑1 (MQ=255)

                                                           aaGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTtgtg                                                                                        <  1:472160/79‑1 (MQ=255)

                                                           aaGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTtgtg                                                                                        >  2:472160/1‑79 (MQ=255)

                                                                gCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                          >  2:34652/1‑120 (MQ=255)

                                                                gCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTa                                          <  1:34652/120‑1 (MQ=255)

                                                                 cAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAAt                      <  1:205639/139‑1 (MQ=255)

                                                                 cAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAAt                      <  1:274838/139‑1 (MQ=255)

                                                                        cacacaGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                            <  2:589244/78‑1 (MQ=255)

                                                                        cacacaGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                            >  1:589244/1‑78 (MQ=255)

                                                                         acacaGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                    >  1:396730/1‑117 (MQ=255)

                                                                         acacaGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGt                                    <  2:396730/117‑1 (MQ=255)

                                                                          cacaGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAg             <  2:1321652/139‑1 (MQ=255)

                                                                           acaGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                            >  2:1005189/1‑75 (MQ=255)

                                                                           acaGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                            <  1:1005189/75‑1 (MQ=255)

                                                                           acaGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCagaaaaga                                                    <  1:949927/99‑1 (MQ=255)

                                                                           acaGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCagaaaaga                                                    >  2:949927/1‑99 (MQ=255)

                                                                               aaaGCCTGGAATTGTCGTCACTCCAGACAGTCAAACTATAAAAAAAGTTAACCCTTGTGGa                                                                                      >  2:869904/1‑61 (MQ=37)

                                                                               aaaGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGa                                                                                      <  1:869904/61‑1 (MQ=255)

                                                                                   ccTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAAt                      >  1:413122/1‑121 (MQ=255)

                                                                                   ccTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAAt                      <  2:413122/121‑1 (MQ=255)

                                                                                     tGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAatga  >  1:694734/1‑139 (MQ=255)

                                                                                            gtcgtcACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGt                                           <  2:1369674/91‑1 (MQ=255)

                                                                                            gtcgtcACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGt                                           >  1:1369674/1‑91 (MQ=255)

                                                                                                 cACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTaaa                                        >  2:785947/1‑89 (MQ=255)

                                                                                                 cACTCCAGACAGTCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTaaa                                        <  1:785947/89‑1 (MQ=255)

                                                                                                             tCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAAt                                                                          <  1:1165186/43‑1 (MQ=255)

                                                                                                             tCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAAt                                                                          >  2:1165186/1‑43 (MQ=255)

                                                                                                             tCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTa                                   <  1:1235149/82‑1 (MQ=255)

                                                                                                             tCAAACGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTa                                   >  2:1235149/1‑82 (MQ=255)

                                                                                                                  cGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                            <  2:399680/36‑1 (MQ=255)

                                                                                                                  cGATAAAAAAAGATAACCCTTGTGGAGGTTCCTGCa                                                                            >  1:399680/1‑36 (MQ=255)

AAAACCCGACAGATGTGACGTACTTCAGGAACTAATTGATTGTGGTGATATTAGTGCTAAAGATGCAAAAAGCACACAGAAAGCCTGGAATTGTCGTCACTCCAGACAGTCAAACGATAAAAAAAGATAGCCCTTGTGGAGGTTCCTGCAATGTCAAATACATACCAGAAAAGAAAGGCAAGTAAAGAGTATGGTTTATATAATCAATGTAAGAAACTAAATGA  >  NC_000913/3766187‑3766410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A