Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,035,546	T→C	T246A (ACG→GCG)	prfB ←	peptide chain release factor RF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,035,546	0	T	C	100.0%	253.6 / NA	71	T246A (ACG→GCG)	prfB	peptide chain release factor RF‑2
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (37/34); total (37/34)

TTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCA  >  NC_000913/3035416‑3035677

ttCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATaaa                                                                                                                             <  2:28310/139‑1 (MQ=255)

  cATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATAGGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACg                                                                                                                           >  2:795886/1‑139 (MQ=255)

  cATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACg                                                                                                                           >  2:559065/1‑139 (MQ=255)

   aTGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGt                                                                                                                          <  1:559065/139‑1 (MQ=255)

    tGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTc                                                                                                                         >  1:1087837/1‑139 (MQ=255)

    tGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTc                                                                                                                         >  2:414296/1‑139 (MQ=255)

     ggCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCa                                                                                                                        <  1:1175731/139‑1 (MQ=255)

                   tCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATccgc                                                                                                          <  1:414296/139‑1 (MQ=255)

                     ttGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATccgccg                                                                                                        <  1:1129119/139‑1 (MQ=255)

                       gtgCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGt                                                                                                                          <  1:921331/119‑1 (MQ=255)

                       gtgCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGt                                                                                                                          >  2:921331/1‑119 (MQ=255)

                       gtgCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCggg                                                                                                      >  2:573718/1‑139 (MQ=255)

                         gCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGtt                                                                                                    <  1:1194320/139‑1 (MQ=255)

                             ggAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGAtt                                                                                                >  2:390358/1‑139 (MQ=255)

                                 cGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGa                                                                                            <  1:1189300/139‑1 (MQ=255)

                                 cGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGa                                                                                            <  2:1054527/139‑1 (MQ=255)

                                 agacagTTCTGGCACTGGGTCACGATGCCGGTCGGGACGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGa                                                                                            <  2:836858/134‑1 (MQ=255)

                                  ggTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGat                                                                                           <  1:390358/139‑1 (MQ=255)

                                  ggTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGat                                                                                           <  2:1003768/139‑1 (MQ=255)

                                    tCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatat                                                                                         >  2:245107/1‑139 (MQ=255)

                                                 gggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATatcatcatc                                                                            <  2:1087837/139‑1 (MQ=255)

                                                  ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGt                                                                                                                          <  1:1061580/92‑1 (MQ=255)

                                                  ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGt                                                                                                                          >  2:1061580/1‑92 (MQ=255)

                                                  ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaatatca                                                                                 <  2:1157499/133‑1 (MQ=255)

                                                  ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaatatca                                                                                 >  1:1157499/1‑133 (MQ=255)

                                                  ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGa                                                                                            >  1:459700/1‑122 (MQ=255)

                                                  ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGa                                                                                            <  2:459700/122‑1 (MQ=255)

                                                  ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATAtcatcatca                                                                           >  1:1106207/1‑139 (MQ=255)

                                                  ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATAtcatcatca                                                                           >  1:749795/1‑139 (MQ=255)

                                                  ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCACCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATAtcatcatca                                                                           >  1:964286/1‑139 (MQ=255)

                                                  ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACAGCAGATTCGGAACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaatatcactgtct                                                                           >  1:836858/1‑133 (MQ=255)

                                                      aCGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATg                                                                                                                     <  2:1091158/93‑1 (MQ=255)

                                                      aCGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATg                                                                                                                     >  1:1091158/1‑93 (MQ=255)

                                                                  gggATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGata                                                                                          >  1:1282143/1‑108 (MQ=255)

                                                                  gggATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGata                                                                                          <  2:1282143/108‑1 (MQ=255)

                                                                    gATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCTCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATaaacaaac                                                         >  1:694550/1‑139 (MQ=255)

                                                                        tgGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCa                                                                                                                        >  2:622854/1‑72 (MQ=255)

                                                                        tgGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCa                                                                                                                        <  1:622854/72‑1 (MQ=255)

                                                                          ggTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAg                                                   >  2:438232/1‑139 (MQ=255)

                                                                              aTACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGa                                               >  2:72862/1‑139 (MQ=255)

                                                                               tACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGat                                                                                           <  1:1233301/94‑1 (MQ=255)

                                                                               tACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGat                                                                                           >  2:1233301/1‑94 (MQ=255)

                                                                                    aCCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATaaa                                                                                                                             >  1:370765/1‑55 (MQ=255)

                                                                                    aCCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATaaa                                                                                                                             <  2:370765/55‑1 (MQ=255)

                                                                                    aCCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAacgacg                                         >  1:261871/1‑139 (MQ=255)

                                                                                     ccGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGAtt                                                                                                >  2:1099731/1‑83 (MQ=255)

                                                                                     ccGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGAtt                                                                                                <  1:1099731/83‑1 (MQ=255)

                                                                                         aGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCa                                                                                                                        >  2:711889/1‑55 (MQ=255)

                                                                                         aGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCa                                                                                                                        <  1:711889/55‑1 (MQ=255)

                                                                                                gTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCgacgac                             >  1:431499/1‑139 (MQ=255)

                                                                                                   cGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGAccgc                          <  1:245107/139‑1 (MQ=255)

                                                                                                       tAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGa                      >  1:707294/1‑139 (MQ=255)

                                                                                                           gTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCggg                                                                                                      >  1:1280701/1‑55 (MQ=255)

                                                                                                           gTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCggg                                                                                                      <  2:1280701/55‑1 (MQ=255)

                                                                                                             gCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTc                                                                                              <  1:574281/61‑1 (MQ=255)

                                                                                                             gCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTc                                                                                              >  2:574281/1‑61 (MQ=255)

                                                                                                             gCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCaaa                <  2:707294/139‑1 (MQ=255)

                                                                                                              cTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaata                                                                                    <  1:1053261/70‑1 (MQ=255)

                                                                                                              cTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaata                                                                                    >  2:1053261/1‑70 (MQ=255)

                                                                                                              cTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCaaaa               >  2:126782/1‑138 (MQ=255)

                                                                                                              cTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAAc               >  1:1180653/1‑139 (MQ=255)

                                                                                                              cTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAAc               >  2:1207688/1‑139 (MQ=255)

                                                                                                               tGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGCTCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAg                                                   >  2:81088/1‑102 (MQ=255)

                                                                                                               tGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAg                                                   <  1:81088/102‑1 (MQ=255)

                                                                                                                gACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGat                                                                                           >  1:24403/1‑61 (MQ=255)

                                                                                                                gACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGat                                                                                           <  2:24403/61‑1 (MQ=255)

                                                                                                                 aCCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACggg            <  1:675025/139‑1 (MQ=255)

                                                                                                                     cccgcGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCttt        <  2:1106207/139‑1 (MQ=255)

                                                                                                                           ccGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCa  <  1:573718/139‑1 (MQ=255)

                                                                                                                           ccGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCACCATCAACTTCCGGATAAACAAAcgcg                                                      >  1:86568/1‑87 (MQ=255)

                                                                                                                           ccGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCACCATCAACTTCCGGATAAACAAAcgcg                                                      <  2:86568/87‑1 (MQ=255)

TTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCA  >  NC_000913/3035416‑3035677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A