Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I2 R1 765 53.2 2691310 93.4% 2513683 103.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,035,546 T→C T246A (ACG→GCG)  prfB ← peptide chain release factor RF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,035,5460TC100.0% 146.5 / NA 41T246A (ACG→GCG) prfBpeptide chain release factor RF‑2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/20);  total (21/20)

GTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGC  >  NC_000913/3035433‑3035676
                                                                                                                 |                                                                                                                                  
gTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATcc                                                                                                           >  2:305805/1‑139 (MQ=255)
     tgtgCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATgcg                                                                                                                  >  2:199913/1‑127 (MQ=255)
     tgtgCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATgcg                                                                                                                  <  1:199913/127‑1 (MQ=255)
             gAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGAttt                                                                                              <  2:386256/139‑1 (MQ=255)
                 ggTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATgcg                                                                                                                  <  2:469294/115‑1 (MQ=255)
                 ggTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATgcg                                                                                                                  >  1:469294/1‑115 (MQ=255)
                   tCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCa                                                                                                                >  1:832065/1‑115 (MQ=255)
                   tCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCa                                                                                                                <  2:832065/115‑1 (MQ=255)
                   tCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCggg                                                                                                     >  2:1300079/1‑126 (MQ=255)
                   tCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCggg                                                                                                     <  1:1300079/126‑1 (MQ=255)
                   tCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatat                                                                                        <  1:305805/139‑1 (MQ=255)
                        cTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATccgcc                                                                                                        >  1:1261410/1‑118 (MQ=255)
                        cTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATccgcc                                                                                                        <  2:1261410/118‑1 (MQ=255)
                        cTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTc                                                                                             <  1:517813/129‑1 (MQ=255)
                        cTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTc                                                                                             >  2:517813/1‑129 (MQ=255)
                        cTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaata                                                                                   <  1:708687/139‑1 (MQ=255)
                                 ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGa                                                                                           <  2:950657/122‑1 (MQ=255)
                                 ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGa                                                                                           >  1:950657/1‑122 (MQ=255)
                                 ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATAtcatcatca                                                                          >  1:1339208/1‑139 (MQ=255)
                                 ggTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGCACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATAtcatcatca                                                                          >  2:962475/1‑139 (MQ=255)
                                            cGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCggg                                                                                                     <  1:375256/101‑1 (MQ=255)
                                            cGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCggg                                                                                                     >  2:375256/1‑101 (MQ=255)
                                                         ggTAATACGCACCGCAGATTTGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAg                                                  >  2:976368/1‑139 (MQ=255)
                                                         ggTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCa                                                                                                                       <  1:752297/70‑1 (MQ=255)
                                                         ggTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCa                                                                                                                       >  2:752297/1‑70 (MQ=255)
                                                         ggTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatca                                                                                      >  2:508388/1‑103 (MQ=255)
                                                         ggTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatca                                                                                      <  1:508388/103‑1 (MQ=255)
                                                                cGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAacg                                           <  2:1339208/139‑1 (MQ=255)
                                                                   aCCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCa                                                                                                                       <  1:1306119/60‑1 (MQ=255)
                                                                   aCCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCa                                                                                                                       >  2:1306119/1‑60 (MQ=255)
                                                                               gTACGGTTAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCgacgac                            >  1:951782/1‑139 (MQ=255)
                                                                                     ttAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGat                                                                                          >  1:815801/1‑71 (MQ=255)
                                                                                     ttAACGTGCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGat                                                                                          <  2:815801/71‑1 (MQ=255)
                                                                                            gCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATg                                                                                                                    >  1:895272/1‑38 (MQ=255)
                                                                                            gCTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATg                                                                                                                    <  2:895272/38‑1 (MQ=255)
                                                                                             cTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGAt                                                                                                             >  1:635271/1‑44 (MQ=255)
                                                                                             cTGACCGCCCGCGCCGGACGCGCGATAAACGTCAATGCGCAGAt                                                                                                             <  2:635271/44‑1 (MQ=255)
                                                                                                   gcccgcGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaat                                                                                    >  2:546599/1‑63 (MQ=255)
                                                                                                   gcccgcGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGatatcaat                                                                                    <  1:546599/63‑1 (MQ=255)
                                                                                                     ccgcGCCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCtttt      >  1:1105332/1‑139 (MQ=255)
                                                                                                         gcCGGACGCGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGc  <  1:976368/139‑1 (MQ=255)
                                                                                                                 |                                                                                                                                  
GTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGATCCGCCGGGTTGATTTCGATATCAATATCATCATCAACTTCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGC  >  NC_000913/3035433‑3035676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A