Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,078,852	T→C	N3S (AAC→AGC)	cmtA ←	putative mannitol‑specific PTS IIB and IIC components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,078,852	0	T	C	97.4%	273.9 / ‑2.9	78	N3S (AAC→AGC)	cmtA	putative mannitol‑specific PTS IIB and IIC components
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (34/42); total (35/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.71e-01

ATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCT  >  NC_000913/3078716‑3078984

aTGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGctt                                                                                                                                    >  2:377919/1‑139 (MQ=255)

    tCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCa                                                                                                                                <  1:1225667/139‑1 (MQ=255)

            cAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAAtatta                                                                                                                        <  1:797/139‑1 (MQ=255)

              gCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATc                                                                                                                      <  2:834762/139‑1 (MQ=255)

              gCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATc                                                                                                                      <  1:305177/139‑1 (MQ=255)

              gCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATc                                                                                                                      <  1:377919/139‑1 (MQ=255)

                      gTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTagag                                                                                                              <  1:617505/139‑1 (MQ=255)

                        gggAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAgagga                                                                                                            <  1:123435/139‑1 (MQ=255)

                         ggAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCataata                                                                                                                           <  1:1126206/123‑1 (MQ=255)

                         ggAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCataata                                                                                                                           >  2:1126206/1‑123 (MQ=255)

                                 taaCGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAAt                                                                                                   >  1:191974/1‑139 (MQ=255)

                                      cAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATcc                                                                                              <  1:301847/139‑1 (MQ=255)

                                                   ccAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATg                                                                                 >  2:52290/1‑139 (MQ=255)

                                                   ccAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATg                                                                                 >  1:340269/1‑139 (MQ=255)

                                                     aGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCcg                                                                                             >  1:194761/1‑125 (MQ=255)

                                                     aGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCcg                                                                                             <  2:194761/125‑1 (MQ=255)

                                                      ggCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGgaga                                                                                                          >  2:1020056/1‑111 (MQ=255)

                                                      ggCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGgaga                                                                                                          <  1:1020056/111‑1 (MQ=255)

                                                       gCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAAtatta                                                                                                                        <  2:110994/96‑1 (MQ=255)

                                                       gCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAAtatta                                                                                                                        >  1:110994/1‑96 (MQ=255)

                                                       gCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCg                                                                             <  1:829373/139‑1 (MQ=255)

                                                           tAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTaa                                                                         <  2:191974/139‑1 (MQ=255)

                                                               aGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGt                                                                     <  2:340269/139‑1 (MQ=255)

                                                                gCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTa                                                                                                                 <  2:453608/94‑1 (MQ=255)

                                                                gCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTa                                                                                                                 >  1:453608/1‑94 (MQ=255)

                                                                           ggggATGACGATTGCAGTCAAAATGCCCCCAAAAGCCTGGATCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAACTATCCGCGGCTGATAATGTCCGCTAAGTGTTTTTCTgatgat                                                         <  2:774344/139‑1 (MQ=255)

                                                                           ggggATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCcgc                                                                                            >  2:1265466/1‑104 (MQ=255)

                                                                           ggggATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCcgc                                                                                            <  1:1265466/104‑1 (MQ=255)

                                                                            gggATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCcgc                                                                                            >  1:96545/1‑103 (MQ=255)

                                                                            gggATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCcgc                                                                                            <  2:96545/103‑1 (MQ=255)

                                                                            gggATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGatgatg                                                        <  2:146559/139‑1 (MQ=255)

                                                                            gggATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGatgatg                                                        <  1:1213871/139‑1 (MQ=255)

                                                                            gggATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGatgatg                                                        >  1:146559/1‑139 (MQ=255)

                                                                              gATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCCCGAGCAGACTTGCTTTCCATAATATTATCCTGTagag                                                                                                              >  2:758682/1‑83 (MQ=255)

                                                                              gATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTagag                                                                                                              <  1:758682/83‑1 (MQ=255)

                                                                                 gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCa                                                                                                                                <  2:96365/62‑1 (MQ=255)

                                                                                 gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCa                                                                                                                                >  1:96365/1‑62 (MQ=255)

                                                                                 gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGtt                                                   <  1:1192247/139‑1 (MQ=255)

                                                                                 gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGtt                                                   >  2:596768/1‑139 (MQ=255)

                                                                                 gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGtt                                                   >  1:1191838/1‑139 (MQ=255)

                                                                                 gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGtt                                                   >  2:395297/1‑139 (MQ=255)

                                                                                  aCCATTGCAGTCAAAAAGCCCTCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCataata                                                                                                                           <  2:928336/66‑1 (MQ=255)

                                                                                  aCCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCataata                                                                                                                           >  1:928336/1‑66 (MQ=255)

                                                                                  aCCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTa                                                  >  1:524225/1‑139 (MQ=255)

                                                                                  aCCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTa                                                  >  2:298906/1‑139 (MQ=255)

                                                                                   ccATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTaa                                                 >  1:586027/1‑139 (MQ=255)

                                                                                      ttGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGatgatg                                                        <  1:1203559/129‑1 (MQ=255)

                                                                                      ttGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGatgatg                                                        >  2:1203559/1‑129 (MQ=255)

                                                                                       tGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAgt                                             >  1:1105061/1‑139 (MQ=255)

                                                                                          aGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAgttgt                                          <  1:52290/139‑1 (MQ=255)

                                                                                           gTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAgttgtt                                         >  1:1030027/1‑139 (MQ=255)

                                                                                            tCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCata                                                                                                                              >  2:1095396/1‑53 (MQ=255)

                                                                                            tCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCata                                                                                                                              <  1:1095396/53‑1 (MQ=255)

                                                                                            tCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAgagga                                                                                                            >  2:974996/1‑71 (MQ=255)

                                                                                            tCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAgagga                                                                                                            <  1:974996/71‑1 (MQ=255)

                                                                                            tCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTaa                                                                                                    >  1:447099/1‑79 (MQ=255)

                                                                                            tCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTaa                                                                                                    <  2:447099/79‑1 (MQ=255)

                                                                                             cAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCg                                       <  1:395297/139‑1 (MQ=255)

                                                                                             cAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTGTTTCTGATGATGCTGTTAAGAGTTGTTct                                       <  2:524225/139‑2 (MQ=255)

                                                                                               aaaaGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAg                                     <  1:298906/139‑1 (MQ=255)

                                                                                                      cccAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGatgatg                                                        <  1:1307321/113‑1 (MQ=255)

                                                                                                      cccAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGACAATGTCCGCTAATTGTTTTTCTGatgatg                                                        >  2:1307321/1‑113 (MQ=255)

                                                                                                              ccTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGca                      <  2:1191838/139‑1 (MQ=255)

                                                                                                                  gACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATttcttct                           <  2:477820/130‑1 (MQ=255)

                                                                                                                  gACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATttcttct                           >  1:477820/1‑130 (MQ=255)

                                                                                                                      tttGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAAtt              <  2:586027/139‑1 (MQ=255)

                                                                                                                       ttGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCg                        <  1:426073/128‑1 (MQ=255)

                                                                                                                       ttGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCg                        >  2:426073/1‑128 (MQ=255)

                                                                                                                        tGCACGAGCAGACTTTCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTGATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAg                                     <  1:879518/114‑1 (MQ=255)

                                                                                                                        tGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGatgatg                                                        <  2:759106/95‑1 (MQ=255)

                                                                                                                        tGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGatgatg                                                        >  1:759106/1‑95 (MQ=255)

                                                                                                                        tGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTaga                                                                                                               <  1:425343/40‑1 (MQ=255)

                                                                                                                        tGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTaga                                                                                                               >  2:425343/1‑40 (MQ=255)

                                                                                                                        tGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAg                                     >  2:879518/1‑114 (MQ=255)

                                                                                                                            cGAGCAGACTTACTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCt                                                                           >  2:339270/1‑72 (MQ=255)

                                                                                                                            cGAGCAGACTTACTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCt                                                                           <  1:339270/72‑1 (MQ=255)

                                                                                                                                  gACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGca                      >  1:844711/1‑119 (MQ=255)

                                                                                                                                  gACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCt  >  1:839943/1‑139 (MQ=255)

                                                                                                                                  gACTTGCTTTCCATAATATTATCCTCTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTTGca                      <  2:844711/119‑1 (MQ=255)

ATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCT  >  NC_000913/3078716‑3078984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A