Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I2 R1 766 55.6 2631458 94.7% 2491990 109.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,664,986 C→T *275* (TAG→TAA)  gadX ← acid resistance regulon transcriptional activator, autoactivator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,664,9860CT100.0% 183.3 / NA 58*275* (TAG→TAA) gadXacid resistance regulon transcriptional activator, autoactivator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/26);  total (32/26)

GTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTCTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTCCTGATACTCTGTGGGCGTCATCCCA  >  NC_000913/3664850‑3665115
                                                                                                                                         |                                                                                                                                 
gTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTta                                                                                                                                 <  2:443635/139‑1 (MQ=255)
   atatTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTta                                                                                                                                 >  1:721218/1‑136 (MQ=255)
       ttAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCtt                                                                                                                          <  2:1153440/139‑1 (MQ=255)
       ttAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCtt                                                                                                                          <  2:503002/139‑1 (MQ=255)
         aaCTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTAt                                                                                                                        >  1:820346/1‑139 (MQ=255)
           cTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTAttc                                                                                                                      >  1:1029780/1‑139 (MQ=255)
              aCTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGGTTTTTTTTTATGTCTGAGTAAAACTTTATAATCTTAttcct                                                                                                                    >  1:417620/1‑139 (MQ=255)
                    agCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAg                                                                                                             >  2:792159/1‑139 (MQ=255)
                    agCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAg                                                                                                             >  2:1081203/1‑139 (MQ=255)
                            gTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCa                                                                                                     >  1:1292881/1‑139 (MQ=255)
                            gTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCa                                                                                                     <  2:793933/139‑1 (MQ=255)
                              ttCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGt                                                                                                   >  1:533865/1‑139 (MQ=255)
                                cccGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGc                                                                                                 >  1:430192/1‑139 (MQ=255)
                                 ccGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGcc                                                                                                <  2:430192/139‑1 (MQ=255)
                                     gctaaCAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAACCTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTaa                                                                                            <  1:152181/136‑1 (MQ=255)
                                       cAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTaaaa                                                                                          >  1:358004/1‑139 (MQ=255)
                                                gtgtTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTg                                                                                 <  2:358004/139‑1 (MQ=255)
                                                         cGGCTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAACACTTTATAATCTTATTCGTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAAt                                                                        >  2:844431/1‑139 (MQ=255)
                                                             ttattaGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACtt                                                                    >  1:960262/1‑139 (MQ=255)
                                                             ttattaGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACtt                                                                    >  1:287189/1‑139 (MQ=255)
                                                              tattaGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTg                                                                   >  1:1118837/1‑139 (MQ=255)
                                                                  aGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTgccgc                                                               <  2:820346/139‑1 (MQ=255)
                                                                      tGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAg                                                           >  2:1227294/1‑139 (MQ=255)
                                                                           aCGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACg                                                      <  2:1029780/139‑1 (MQ=255)
                                                                             ggCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGt                                                    >  1:859940/1‑139 (MQ=255)
                                                                                  aCTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGAc                                               <  1:792159/139‑1 (MQ=255)
                                                                                       ccTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAAtct                                          >  2:482606/1‑139 (MQ=255)
                                                                                           ttCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTgc                                      >  1:585847/1‑139 (MQ=255)
                                                                                            tCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTg                                                                                 <  2:468972/95‑1 (MQ=255)
                                                                                            tCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTg                                                                                 >  1:468972/1‑95 (MQ=255)
                                                                                                ggTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGa                                 <  2:859940/139‑1 (MQ=255)
                                                                                                ggTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGa                                 >  2:913494/1‑139 (MQ=255)
                                                                                                ggTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGa                                 >  2:99719/1‑139 (MQ=255)
                                                                                                  tCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATc                               <  2:1228942/139‑1 (MQ=255)
                                                                                                  tCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATc                               <  1:1227294/139‑1 (MQ=255)
                                                                                                  tCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCCCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATc                               <  1:913494/139‑1 (MQ=255)
                                                                                                   ccccTATGCCGGGTTTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATc                               <  2:417620/139‑1 (MQ=255)
                                                                                                    cccTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGc                             <  2:533865/139‑1 (MQ=255)
                                                                                                              gg‑TTTTTTTTATGTCTGATTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGcaa                                                                            <  2:855859/82‑1 (MQ=255)
                                                                                                              gg‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTAttcctt                                                                                                                   <  1:1223732/43‑1 (MQ=255)
                                                                                                              gg‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTAttcctt                                                                                                                   >  2:1223732/1‑43 (MQ=255)
                                                                                                              gg‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGc                             >  2:224554/1‑129 (MQ=255)
                                                                                                              gg‑TTTTTTTTATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGc                             <  1:224554/129‑1 (MQ=255)
                                                                                                              gg‑TTTTTTATATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGcaa                                                                            >  1:855859/1‑82 (MQ=255)
                                                                                                                    tttttATGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTCCTGATACTCtgtg              >  1:296757/1‑139 (MQ=255)
                                                                                                                         aTGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTCCTGATACTCTGTGGGCGt         <  2:287189/139‑1 (MQ=255)
                                                                                                                          tGTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTCCTGATACTCTGTGGGCGTc        >  1:510833/1‑139 (MQ=255)
                                                                                                                           gTCTGAGTAAAACTTTATAATCTTTTTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAGTACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTCCTGATACTCTGTGGGCGTCa       <  1:1076089/139‑1 (MQ=255)
                                                                                                                           gTCTGAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTCCTGATACTCTGTGGGCGTCa       <  2:1292881/139‑1 (MQ=255)
                                                                                                                              tGAGTAAAACTTTATATACTTATTTCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGAAACAATACTTGCAGCCGAGTCACGGTTCGACAATCTCTGCTCGCATCGCTCCTGATACTCTGTGGGCGTCAtac    >  2:702392/1‑137 (MQ=255)
                                                                                                                               gAGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTCCTGATACTCTGTGGGCGTCATccc   >  2:1245359/1‑139 (MQ=255)
                                                                                                                                aGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTg                                                                                 <  1:188588/60‑1 (MQ=255)
                                                                                                                                aGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTg                                                                                 >  2:188588/1‑60 (MQ=255)
                                                                                                                                aGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACg                                                      >  1:187262/1‑87 (MQ=255)
                                                                                                                                aGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACg                                                      <  2:187262/87‑1 (MQ=255)
                                                                                                                                aGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTCCTGATACTCTGTGGGCGt         >  1:916086/1‑132 (MQ=255)
                                                                                                                                aGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTCCTGATACTCTGTGGGCGt         <  2:916086/132‑1 (MQ=255)
                                                                                                                                aGTAAAACTTTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTCCTGATACTCTGTGGGCGTCATCCCa  >  1:1066830/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                                 
GTTATATTTAACTTACTGAGAGCACAAAGTTTCCCGTGCCAACAGGGAGTGTTATAACGGTTTATTAGTCTGGAGACGGCAGACTATCCTCTTCCCGGTCCCCTATGCCGGG‑TTTTTTTTATGTCTGAGTAAAACTCTATAATCTTATTCCTTCCGCAGAACGGTCAGTGCCGTAAAAATTCCCTTGCGCAACAATACTTGCCGCCGAGTCACGGTTCGACAATCTCTGCGCCGATCGCTCCTGATACTCTGTGGGCGTCATCCCA  >  NC_000913/3664850‑3665115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A