Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F1 I1 R1 778 68.7 3228504 93.4% 3015422 109.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,014,287 C→T *257* (TAG→TAA)  yqeC ← putative selenium‑dependent hydroxylase accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,014,2870CT100.0% 219.6 / NA 68*257* (TAG→TAA) yqeCputative selenium‑dependent hydroxylase accessory protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/35);  total (33/35)

GGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCG  >  NC_000913/3014150‑3014396
                                                                                                                                         |                                                                                                             
gggAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACTATTATGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCGCATTGGATAT‑CTTTCAGTAACCCGGAGTACTCGTGCCGTTa                                                                                                             <  1:259424/139‑1 (MQ=255)
       gAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTcaca                                                                                                      >  2:594521/1‑139 (MQ=255)
               gCTATCAACAATTTGCGCCGTAGCAAACGGACAATCTTGCTCTTCGGGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTCACCCGGAATACCCGGGCCGTTCATTCATAAATCTgca                                                                                              >  1:285679/1‑138 (MQ=255)
               gCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcg                                                                                              <  1:845169/139‑1 (MQ=255)
                           ttGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                                  <  1:439591/139‑1 (MQ=255)
                            tGCGCCATAGCAAATGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                                 <  1:124465/139‑1 (MQ=255)
                            tGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                                  <  2:1531484/138‑1 (MQ=255)
                            tGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg                                                                                  >  1:1531484/1‑138 (MQ=255)
                            tGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                                 <  2:1531283/139‑1 (MQ=255)
                              cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                               <  1:773554/139‑1 (MQ=255)
                              cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                               <  1:166700/139‑1 (MQ=255)
                               gcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                                            <  1:852882/125‑1 (MQ=255)
                               gcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                                            >  2:852882/1‑125 (MQ=255)
                               gcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGt                                                                              >  2:311827/1‑139 (MQ=255)
                                    aGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                                 >  2:495986/1‑131 (MQ=255)
                                    aGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa                                                                                 <  1:495986/131‑1 (MQ=255)
                                    aGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTg                                                                         >  1:920531/1‑139 (MQ=255)
                                      cAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGta                                                                       >  1:1085568/1‑139 (MQ=255)
                                           ggACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCg                                                                  <  2:911994/139‑1 (MQ=255)
                                           ggACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCg                                                                  <  1:582596/139‑1 (MQ=255)
                                             aCAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCgac                                                                >  1:324601/1‑137 (MQ=255)
                                               aaTTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCcca                                                              <  2:527158/139‑1 (MQ=255)
                                                          tCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGcc                                                   <  1:311827/139‑1 (MQ=255)
                                                           cGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTg                                                                                      <  2:216939/103‑1 (MQ=255)
                                                           cGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTg                                                                                      >  1:216939/1‑103 (MQ=255)
                                                           cGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCct                                                  >  1:14981/1‑139 (MQ=255)
                                                           cGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCct                                                  >  1:1066675/1‑139 (MQ=255)
                                                                                       cATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTACTTCACAAATCTGCGCGCGATTGCa                                                                                    >  1:932667/1‑77 (MQ=255)
                                                                                       cATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCa                                                                                    <  2:932667/77‑1 (MQ=255)
                                                                                       cATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCa                                                                                    <  1:897597/77‑1 (MQ=255)
                                                                                       cATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCa                                                                                    >  2:897597/1‑77 (MQ=255)
                                                                                       cATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGAt                                                       <  1:1237860/106‑1 (MQ=255)
                                                                                       cATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGAt                                                       >  2:1237860/1‑106 (MQ=255)
                                                                                          aCCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAg                   <  2:920531/139‑1 (MQ=255)
                                                                                           ccAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                                            <  1:241768/65‑1 (MQ=255)
                                                                                           ccAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                                            >  2:241768/1‑65 (MQ=255)
                                                                                             aaCCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                               <  1:906562/76‑1 (MQ=255)
                                                                                             aaCCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg                                                                               >  2:906562/1‑76 (MQ=255)
                                                                                              aCCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCg               >  2:604708/1‑139 (MQ=255)
                                                                                              aCCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCg               <  2:14981/139‑1 (MQ=255)
                                                                                               ccTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGc              >  2:1154937/1‑139 (MQ=255)
                                                                                                   aaTGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcg                                                                                              >  1:88723/1‑55 (MQ=255)
                                                                                                   aaTGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcg                                                                                              <  2:88723/55‑1 (MQ=255)
                                                                                                    aTGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGa                        <  2:311586/124‑1 (MQ=255)
                                                                                                    aTGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGa                        >  1:311586/1‑124 (MQ=255)
                                                                                                           atcTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCct                                                  <  1:848170/90‑1 (MQ=255)
                                                                                                           atcTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCct                                                  >  2:848170/3‑92 (MQ=255)
                                                                                                           atcTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATc   >  2:167568/3‑139 (MQ=255)
                                                                                                           atcTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATc   >  1:335059/3‑139 (MQ=255)
                                                                                                           atcTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATc   >  2:1255371/3‑139 (MQ=255)
                                                                                                            tcTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCg  <  1:167568/138‑1 (MQ=255)
                                                                                                                  aGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                                            <  1:561938/43‑1 (MQ=255)
                                                                                                                  aGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg                                                                                            >  2:561938/1‑43 (MQ=255)
                                                                                                                       cccGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGAt                                                       >  2:1563316/1‑75 (MQ=255)
                                                                                                                       cccGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGAt                                                       <  1:1563316/75‑1 (MQ=255)
                                                                                                                            aaTACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTg                                                                                      <  1:473715/39‑1 (MQ=38)
                                                                                                                            aaTACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTg                                                                                      >  2:473715/1‑39 (MQ=255)
                                                                                                                            aaTACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCg  <  2:552213/123‑1 (MQ=255)
                                                                                                                            aaTACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCg  >  1:552213/1‑123 (MQ=255)
                                                                                                                                    ggCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCg                                                                  <  2:353174/51‑1 (MQ=255)
                                                                                                                                    ggCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCg                                                                  >  1:353174/1‑51 (MQ=255)
                                                                                                                                     gCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCg                                                                  >  1:1465703/1‑50 (MQ=255)
                                                                                                                                     gCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCg                                                                  <  2:1465703/50‑1 (MQ=255)
                                                                                                                                     gCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAg                   <  2:683250/97‑1 (MQ=255)
                                                                                                                                     gCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAg                   >  1:683250/1‑97 (MQ=255)
                                                                                                                                     gCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCg               >  2:1409401/1‑101 (MQ=255)
                                                                                                                                     gCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCg               <  1:1409401/101‑1 (MQ=255)
                                                                                                                                     gCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAAt    >  2:1414538/1‑112 (MQ=255)
                                                                                                                                     gCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAAt    <  1:1414538/112‑1 (MQ=255)
                                                                                                                                         |                                                                                                             
GGGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAATCG  >  NC_000913/3014150‑3014396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A