Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I2 R1
|
760 |
52.7 |
2744488 |
90.2% |
2475528 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,014,287 |
C→T |
*257* (TAG→TAA) |
yqeC ← |
putative selenium‑dependent hydroxylase accessory protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,014,287 | 0 | C | T | 100.0%
| 178.2
/ NA
| 56 | *257* (TAG→TAA) | yqeC | putative selenium‑dependent hydroxylase accessory protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (27/29); total (27/29) |
GGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAA > NC_000913/3014151‑3014393
|
ggAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTaa > 2:82680/1‑139 (MQ=255)
gAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTcaca > 2:623295/1‑139 (MQ=255)
gAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTcaca > 2:233256/1‑139 (MQ=255)
cGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTg > 1:1048359/1‑139 (MQ=255)
gCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcg < 2:449735/139‑1 (MQ=255)
gCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcg > 1:340017/1‑139 (MQ=255)
tATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg < 2:45338/139‑1 (MQ=255)
tATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg < 2:1237844/139‑1 (MQ=255)
caaTTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTg < 2:1211338/139‑1 (MQ=255)
ttGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg < 1:464555/139‑1 (MQ=255)
tGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa < 2:1048359/139‑1 (MQ=255)
tGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa < 2:1133024/139‑1 (MQ=255)
gcgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGAt > 1:1036175/1‑139 (MQ=255)
cgcCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg < 2:139191/139‑1 (MQ=255)
cgcCATAGCAAACGGACAATTGTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCatgatg < 1:1175041/139‑1 (MQ=255)
aCGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTatat < 2:1036175/139‑1 (MQ=255)
aaTTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCcca < 1:233256/139‑1 (MQ=255)
aGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCctct < 1:82680/139‑1 (MQ=255)
tgtgAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGttgttg > 2:1330076/1‑139 (MQ=255)
tgAAAATATTCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCa < 1:623295/139‑1 (MQ=255)
ttCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGcc < 2:243799/112‑1 (MQ=255)
ttCATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGcc > 1:243799/1‑112 (MQ=255)
cATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg < 2:936842/69‑1 (MQ=255)
cATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg > 1:936842/1‑69 (MQ=255)
cATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCa > 1:8357/1‑129 (MQ=255)
cATACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCa < 2:8357/129‑1 (MQ=255)
aTACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAgg > 1:334817/1‑139 (MQ=255)
tACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGa > 1:575449/1‑78 (MQ=255)
tACCAACCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCAAAAATCTGCGCGCGATTGCAGGa < 2:575449/78‑1 (MQ=255)
aCCTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGAGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCg > 2:914611/1‑139 (MQ=255)
ccTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcgc > 2:573829/1‑62 (MQ=255)
ccTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcgc < 1:573829/62‑1 (MQ=255)
ccTCAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGc > 1:863981/1‑139 (MQ=255)
cAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTa > 1:46098/1‑41 (MQ=255)
cAATGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTa < 2:46098/41‑1 (MQ=255)
tGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCACCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCt < 1:452010/130‑1 (MQ=255)
tGGATAT‑CTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCACCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCt > 2:452010/1‑130 (MQ=255)
atatcTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCa < 1:510690/108‑1 (MQ=255)
atatcTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCa > 2:510690/5‑112 (MQ=255)
atatcTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCaa > 1:699157/5‑139 (MQ=255)
tatcTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGAtt < 1:1351132/53‑1 (MQ=255)
tatcTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGAtt > 2:1351132/4‑56 (MQ=255)
atcTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTg < 1:1234670/54‑1 (MQ=255)
atcTTTCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTg > 2:1234670/3‑56 (MQ=255)
tttCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg < 2:1226282/47‑1 (MQ=255)
tttCAGTAACCCGGAATACCCGGGCCGTTAATTCACAAATCTgcgcg > 1:1226282/1‑47 (MQ=255)
aCCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg < 2:443398/52‑1 (MQ=255)
aCCCGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg > 1:443398/1‑52 (MQ=255)
cccGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg < 2:605219/51‑1 (MQ=255)
cccGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg > 1:605219/1‑51 (MQ=255)
cGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg < 2:1212631/49‑1 (MQ=255)
cGGAATACCCGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATg > 1:1212631/1‑49 (MQ=255)
cGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg > 2:741732/1‑37 (MQ=255)
cGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAgg < 1:741732/37‑1 (MQ=38)
cGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGc > 2:797272/1‑101 (MQ=255)
cGGGCCGTTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGc < 1:797272/101‑1 (MQ=255)
|
GGAGTCGAGCCTCGGCTATCAACAATTTGCGCCATAGCAAACGGACAATTTTGCTCTTCGAGTTTTGCAGCCTCTGTGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTTGCAGCGGTTGAAGGAGCTCGCTTTGTGCAA > NC_000913/3014151‑3014393
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
