Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I1 R1	770	68.5	3470224	91.7%	3182195	103.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,587,389	G→A	P242L (CCG→CTG)	ugpQ ←	glycerophosphodiester phosphodiesterase, cytosolic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,587,389	0	G	A	100.0%	107.9 / NA	34	P242L (CCG→CTG)	ugpQ	glycerophosphodiester phosphodiesterase, cytosolic
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/18); total (16/18)

CAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTT  >  NC_000913/3587260‑3587524

cAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCa                                                                                                                                >  2:950723/1‑139 (MQ=255)

  aCCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACg                                                                                                                              >  2:1270998/1‑139 (MQ=255)

          ccGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGc                                                                                                                      <  2:1218790/139‑1 (MQ=255)

                  ttCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTAc                                                                                                              >  1:709940/1‑139 (MQ=255)

                    cGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAg                                                                                                            >  1:549735/1‑139 (MQ=255)

                           tCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAAt                                                                                                     >  2:1023074/1‑139 (MQ=255)

                                    tatGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACAcccc                                                                                            >  2:414528/1‑139 (MQ=255)

                                           ccGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCgcag                                                                                     <  1:950723/139‑1 (MQ=255)

                                                gaggagaAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAAct                                                                                <  2:549735/139‑1 (MQ=255)

                                                           gCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTgcgcgc                                                                     <  1:414528/139‑1 (MQ=255)

                                                           gCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTgcgcgc                                                                     >  1:1591524/1‑139 (MQ=255)

                                                            cGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCt                                                                    <  2:1234241/139‑1 (MQ=255)

                                                                 tGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGt                                                               >  1:1698685/1‑139 (MQ=255)

                                                                        tCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                                                        <  2:1591524/139‑1 (MQ=255)

                                                                        tCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                                                        <  1:1270998/139‑1 (MQ=255)

                                                                          aGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACg                                                      <  1:1625270/139‑1 (MQ=255)

                                                                            cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGa                                                                                                                                       >  1:442062/1‑56 (MQ=255)

                                                                            cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCAt                                                                                                                    >  1:1510207/1‑75 (MQ=255)

                                                                            cccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCAt                                                                                                                    <  2:1510207/75‑1 (MQ=255)

                                                                             ccAGCACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGta                                                   <  2:686751/139‑1 (MQ=255)

                                                                                 cACGCCGCAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATaaa                                               >  1:370028/1‑139 (MQ=255)

                                                                                      cgcAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                                                        <  1:1245300/125‑1 (MQ=255)

                                                                                      cgcAGCCTGACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                                                        >  2:1245300/1‑125 (MQ=255)

                                                                                              gACATTCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAg                                  >  2:1276973/1‑139 (MQ=255)

                                                                                                   tCCGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCgg                             <  1:1276973/139‑1 (MQ=255)

                                                                                                     cGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCgcag                                                                                     <  2:627824/81‑1 (MQ=255)

                                                                                                     cGTTGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCgcag                                                                                     >  1:627824/1‑81 (MQ=255)

                                                                                                       ttGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                                                        >  1:805746/1‑108 (MQ=255)

                                                                                                       ttGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGa                                                        <  2:805746/108‑1 (MQ=255)

                                                                                                        tGAAAATTATTGGGCCGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCt                        <  2:679313/139‑1 (MQ=255)

                                                                                                                      ccGTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCAc          <  1:1023074/139‑1 (MQ=255)

                                                                                                                        gTAAAGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTc        >  2:1363205/1‑139 (MQ=255)

                                                                                                                           aaGTTCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTcgcg     <  1:1363205/139‑1 (MQ=255)

                                                                                                                              ttCAGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCtt  <  2:709940/139‑1 (MQ=255)

CAACCGTAATCCGGATAGTTCGTTGAATCAGCAGCATATGCTGCCGGAGAGGAGAAACGGCGACATGCTGAATCAGCCCAGCACGCCGCAGCCTGACATTCCGTTGAAAACTATTGGGCCGTAAAGTTCGGACCAATCACGTCAATCGCATCGGTACAGATGCAATCCACACCCCAGCGCAGCAACTCTGCTGCGCGCTGGGGTTTATTGACGGTATAAACCAGAATCCGCAGTCCGGCGTCTTTCAACTGCATCACTCGCGCTT  >  NC_000913/3587260‑3587524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A