Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I1 R1
|
759 |
65.2 |
3386260 |
90.7% |
3071337 |
101.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,158,370 |
G→A |
V241I (GTT→ATT) |
yqhG → |
DUF3828 family putative periplasmic protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,158,370 | 0 | G | A | 97.8%
| 141.6
/ ‑4.0
| 46 | V241I (GTT→ATT) | yqhG | DUF3828 family putative periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (25/20); total (26/20) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01 |
TTAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCGTTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAA > NC_000913/3158248‑3158496
|
ttAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTc < 1:900277/135‑1 (MQ=255)
ttAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTc > 2:900277/1‑135 (MQ=255)
cTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTc > 1:1108571/1‑125 (MQ=255)
cTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGaaa > 1:321966/1‑139 (MQ=255)
cTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGAAGAAATTc < 2:1108571/125‑1 (MQ=255)
caccTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGAt < 2:321966/139‑1 (MQ=255)
ccTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTc < 2:234207/119‑1 (MQ=255)
ccTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTc > 1:234207/1‑119 (MQ=255)
ccTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTc > 2:294358/1‑139 (MQ=255)
ccTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTc > 2:217265/1‑139 (MQ=255)
ttCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTccg > 2:676536/1‑139 (MQ=255)
aCGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCa < 1:217265/139‑1 (MQ=255)
gCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGa < 2:1669862/105‑1 (MQ=255)
gCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGa > 1:1669862/1‑105 (MQ=255)
ccGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTccgc > 2:1381774/1‑121 (MQ=255)
ccGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTccgc < 1:1381774/121‑1 (MQ=255)
gATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGa > 1:844044/1‑139 (MQ=255)
gCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAAt < 1:294358/139‑1 (MQ=255)
aGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGcaac > 2:857301/1‑139 (MQ=255)
aaCGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGaa > 2:1421087/1‑85 (MQ=255)
aaCGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGaa < 1:1421087/85‑1 (MQ=255)
ggCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGa < 1:857301/139‑1 (MQ=255)
tttatttaCGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCt > 1:1445153/1‑116 (MQ=255)
tttatttaCGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCt < 2:1445153/116‑1 (MQ=255)
cAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGttt > 1:32038/1‑139 (MQ=255)
gATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAg < 1:676536/139‑1 (MQ=255)
aTCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAg > 1:186222/1‑49 (MQ=255)
aTCTGTTTTGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAg < 2:186222/49‑1 (MQ=255)
ttGGCGAAGAGTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGa > 2:270722/1‑139 (MQ=255)
gagTCGCTATTTTCACGCGTTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAGCAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGt > 1:107752/1‑139 (MQ=255)
gagTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGAt > 2:968768/1‑54 (MQ=255)
gagTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGAt < 1:968768/54‑1 (MQ=255)
gagTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTccgcc > 2:245215/1‑60 (MQ=255)
gagTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTccgcc < 1:245215/60‑1 (MQ=255)
gagTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCAt > 2:200657/1‑65 (MQ=255)
gagTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCAt < 1:200657/65‑1 (MQ=255)
gagTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGca < 1:936157/94‑1 (MQ=255)
gagTCGCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGca > 2:936157/1‑94 (MQ=255)
gCTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGaa > 1:1399781/1‑139 (MQ=255)
cTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAgg < 2:141785/128‑1 (MQ=255)
cTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAgg > 1:141785/1‑128 (MQ=255)
cTATTTTCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGaaa > 2:1435156/1‑139 (MQ=255)
ttCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGa > 1:370093/1‑90 (MQ=255)
ttCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGa < 2:370093/90‑1 (MQ=255)
tCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGa < 1:704522/98‑1 (MQ=255)
tCACGCATTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGa > 2:704522/1‑98 (MQ=255)
|
TTAATGCCTTCTATAAAACCACCTTCAAGACGGCCAATAATCCCGATGAAGATATGCAAATAGAACGGCAATTTATTTACGACAATCCGATCTGTTTTGGCGAAGAGTCGCTATTTTCACGCGTTGATGAAATTCGAGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAA > NC_000913/3158248‑3158496
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
