Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A10 F1 I1 R1 | 759 | 65.2 | 3386260 | 90.7% | 3071337 | 101.6 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,781,017 | G→A | *397* (TAG→TAA) | lldD → | L‑lactate dehydrogenase, FMN‑linked |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,781,017 | 0 | G | A | 100.0% | 204.8 / NA | 63 | *397* (TAG→TAA) | lldD | L‑lactate dehydrogenase, FMN‑linked |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/36); total (27/36) | |||||||||||
AAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGA > NC_000913/3780882‑3781140 | aaaaGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCg < 2:596598/139‑1 (MQ=255) agaTGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTgc > 1:999653/1‑139 (MQ=255) gaTGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTgcg < 1:1159165/139‑1 (MQ=255) aTGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTgcgc > 1:438381/1‑139 (MQ=255) aaGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGccccc < 2:670950/139‑1 (MQ=255) ggCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt > 2:1523018/1‑136 (MQ=255) ggCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt < 1:1523018/136‑1 (MQ=255) cGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCAccc > 2:69077/1‑139 (MQ=255) gATGACGCTGACTAGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGTCACTGGCTCCCATGGCTAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCt < 2:1117108/139‑1 (MQ=255) tGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATAGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTaa < 2:1200611/139‑1 (MQ=255) tGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCctct > 2:1401512/1‑139 (MQ=255) gcgcGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCa < 1:1398674/139‑1 (MQ=255) cgcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAg < 1:1401512/139‑1 (MQ=255) cgcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCCCCCATGGCGAAAGGGAATGCGGCATAATCGTTTgc > 1:951236/1‑111 (MQ=255) cgcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCCCCCATGGCGAAAGGGAATGCGGCATAATCGTTTgc < 2:951236/111‑1 (MQ=255) cgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTgcg > 2:1084491/1‑110 (MQ=255) cgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTgcg < 1:1084491/110‑1 (MQ=255) cGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGtt < 1:69077/139‑1 (MQ=255) tACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcgc < 1:1369750/139‑1 (MQ=255) aCGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCt < 2:1464319/139‑1 (MQ=255) gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg < 2:623137/135‑1 (MQ=255) gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg > 1:623137/1‑135 (MQ=255) gATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTc < 1:381042/127‑1 (MQ=255) gATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTc > 2:381042/1‑127 (MQ=255) cAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCctct < 1:1054759/92‑1 (MQ=255) cAGGGGCTGGGTAAAGAGCTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCctct > 2:1054759/1‑92 (MQ=255) cTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAAc < 1:1438464/139‑1 (MQ=255) gggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg > 2:150683/1‑111 (MQ=255) gggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg < 1:150683/111‑1 (MQ=255) gTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCa < 2:447230/126‑1 (MQ=255) gTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCa > 1:447230/1‑126 (MQ=255) aaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCActc > 1:1144671/1‑126 (MQ=255) aaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCActc < 2:1144671/126‑1 (MQ=255) aaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCg < 2:438381/139‑1 (MQ=255) agTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCAc < 2:999653/139‑1 (MQ=255) tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTccc < 2:1652772/76‑1 (MQ=255) tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTccc > 1:1652772/1‑76 (MQ=255) gCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtt > 2:4373/1‑139 (MQ=255) tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt > 1:1625646/1‑55 (MQ=255) tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCt < 2:1625646/55‑1 (MQ=255) gCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCg < 1:218137/139‑1 (MQ=255) gCTCCCATGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCg < 1:4373/139‑1 (MQ=255) tGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcgc > 1:1471736/1‑80 (MQ=255) tGGCGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcgc < 2:1471736/80‑1 (MQ=255) cGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGtt > 2:1329175/1‑71 (MQ=255) cGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGtt < 1:1329175/71‑1 (MQ=255) cGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCa > 2:1634940/1‑111 (MQ=255) cGAAAGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCa < 1:1634940/111‑1 (MQ=255) aaaGGGAATGTGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTc > 1:261016/1‑52 (MQ=25) aaaGGGAATGTGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTc < 2:261016/52‑1 (MQ=25) aaaGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCg > 2:1321844/1‑71 (MQ=255) aaaGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCg < 1:1321844/71‑1 (MQ=255) aGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTccc > 2:851484/1‑86 (MQ=255) aGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTccc < 1:851484/86‑1 (MQ=255) aGGGAATGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga > 1:481232/1‑139 (MQ=255) aaTGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTc < 1:763689/46‑1 (MQ=255) aaTGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTc > 2:763689/1‑46 (MQ=255) aTGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg > 1:1673092/1‑67 (MQ=255) aTGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg < 2:1673092/67‑1 (MQ=255) tGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCa > 1:952121/1‑101 (MQ=255) tGCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCa < 2:952121/101‑1 (MQ=255) gCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg > 2:509212/1‑65 (MQ=255) gCGGCATAATCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg < 1:509212/65‑1 (MQ=255) | AAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGA > NC_000913/3780882‑3781140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |