Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	759	65.2	3386260	90.7%	3071337	101.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,789,352	G→A	R244C (CGC→TGC)	waaH ←	LPS(HepIII)‑glucuronic acid glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,789,352	0	G	A	100.0%	168.7 / NA	52	R244C (CGC→TGC)	waaH	LPS(HepIII)‑glucuronic acid glycosyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/30); total (22/30)

GTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTG  >  NC_000913/3789217‑3789420

gTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                   <  2:9793/139‑1 (MQ=255)

gTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                   <  1:1251713/139‑1 (MQ=255)

gTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                   <  1:1242653/139‑1 (MQ=255)

        gTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                   <  1:608188/131‑1 (MQ=255)

        gTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                   >  2:608188/1‑131 (MQ=255)

        gTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCt                                                              >  1:264459/1‑136 (MQ=255)

        gTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCt                                                              <  2:264459/136‑1 (MQ=255)

            gAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAAtata                                                       <  2:1486429/139‑1 (MQ=255)

              atatCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGt                                                     >  1:1208850/1‑139 (MQ=255)

                      ctctTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTg                                              >  1:740476/1‑138 (MQ=255)

                      ctctTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTg                                              <  2:740476/138‑1 (MQ=255)

                          ttGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAAt                                         >  1:890544/1‑139 (MQ=255)

                           tGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAAtt                                        <  2:1408240/139‑1 (MQ=255)

                           tGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAAtt                                        >  1:189569/1‑139 (MQ=255)

                           tGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAAtt                                        >  1:774287/1‑139 (MQ=255)

                                    cATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                   >  2:374640/1‑103 (MQ=255)

                                    cATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                   <  1:374640/103‑1 (MQ=255)

                                    cATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGttttt                               <  1:1341613/139‑1 (MQ=255)

                                       ggCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                   <  2:16804/100‑1 (MQ=255)

                                       ggCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                   >  1:16804/1‑100 (MQ=255)

                                         cAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTcc                          <  2:1495968/139‑1 (MQ=255)

                                          aaaCACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTccc                         <  2:504395/139‑1 (MQ=255)

                                            acacGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                   <  1:421229/95‑1 (MQ=255)

                                            acacGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                   >  2:421229/1‑95 (MQ=255)

                                            acacGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCtt                       <  2:189569/139‑1 (MQ=255)

                                              acGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGt                     <  1:416159/139‑1 (MQ=255)

                                                 cAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTa                  >  2:1151175/1‑139 (MQ=255)

                                                  aaTGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCGAt                 >  2:1120928/1‑139 (MQ=255)

                                                        tCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTg                                                    <  1:110806/98‑1 (MQ=255)

                                                        tCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTg                                                    >  2:110806/1‑98 (MQ=255)

                                                          gTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                   <  2:1376764/81‑1 (MQ=255)

                                                          gTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                   >  1:1376764/1‑81 (MQ=255)

                                                            aaGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACt       <  1:1151175/139‑1 (MQ=255)

                                                              gTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCa     <  2:1208850/139‑1 (MQ=255)

                                                              gTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCa     <  2:774287/139‑1 (MQ=255)

                                                              gTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCAATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCa     <  1:1120928/139‑1 (MQ=255)

                                                                tATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTTGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTa                  <  1:89477/124‑1 (MQ=255)

                                                                tATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTTGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTa                  >  2:89477/1‑124 (MQ=255)

                                                                tATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACt   >  2:1133035/1‑139 (MQ=255)

                                                                 aTTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGGATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGCTAATTTAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTg  <  1:1133035/139‑1 (MQ=255)

                                                                 aTTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                   >  1:419467/1‑74 (MQ=255)

                                                                 aTTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGt                                                                   <  2:419467/74‑1 (MQ=255)

                                                                                              aaTTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCa     <  1:1105184/107‑1 (MQ=255)

                                                                                              aaTTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACATTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCa     >  2:1105184/1‑107 (MQ=255)

                                                                                                   tGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTg                                                    <  2:724283/55‑1 (MQ=255)

                                                                                                   tGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTg                                                    >  1:724283/1‑55 (MQ=255)

                                                                                                              tttCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAAtata                                                       >  2:803886/1‑41 (MQ=255)

                                                                                                              tttCGATTTAATTTCTCCAGCAGGCAGGTAATCTTAAtata                                                       <  1:803886/41‑1 (MQ=255)

                                                                                                                   aTTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCa     >  2:402777/1‑86 (MQ=255)

                                                                                                                   aTTTAATTTCTCCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCa     <  1:402777/86‑1 (MQ=255)

                                                                                                                            ctcCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTg  <  1:1678003/80‑1 (MQ=255)

                                                                                                                            ctcCAGCAGGCAGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTg  >  2:1678003/1‑80 (MQ=255)

                                                                                                                                  caggcaggTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCtt                       >  1:1676451/1‑53 (MQ=255)

                                                                                                                                  caggcaggTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCtt                       <  2:1676451/53‑1 (MQ=255)

GTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTG  >  NC_000913/3789217‑3789420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A