Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	759	65.2	3386260	90.7%	3071337	101.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,852,890	C→T	33 (TAG→TAA)	ivbL ←	ilvB operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,852,890	0	C	T	100.0%	92.6 / NA	30	33 (TAG→TAA)	ivbL	ilvB operon leader peptide
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/15); total (15/15)

CCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCA‑CCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTA  >  NC_000913/3852771‑3853024

cccGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCcgacga                                                                                                                      >  2:1566149/1‑139 (MQ=255)

cccGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCcgacga                                                                                                                      >  2:396090/1‑139 (MQ=255)

  cGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGacc                                                                                                                    >  2:546968/1‑139 (MQ=255)

    aaCTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGaccac                                                                                                                  <  1:546968/139‑1 (MQ=255)

        tGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGaccacca‑cc                                                                                                             <  1:1688864/139‑1 (MQ=255)

        tGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGaccacca‑cc                                                                                                             <  1:85450/139‑1 (MQ=255)

           cATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCTCCA‑Ccaca                                                                                                          <  2:1575739/139‑1 (MQ=255)

                          ttcttcTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCcgac                                                                                                                        <  1:2335/111‑1 (MQ=255)

                          ttcttcTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCcgac                                                                                                                        >  2:2335/1‑111 (MQ=255)

                            cttctGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCA‑CCACACGCACGACGACCACTgc                                                                                         >  2:1079173/1‑139 (MQ=255)

                                  ggCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCACATTGCCGACGACCACCA‑CCAcacgc                                                                                                       >  2:403668/1‑119 (MQ=255)

                                  ggCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCACATTGCCGACGACCACCA‑CCAcacgc                                                                                                       <  1:403668/119‑1 (MQ=255)

                                                gAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCA‑CCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGgta                                                                     <  1:396090/139‑1 (MQ=255)

                                                      cTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCA‑CCACACGCACgacgac                                                                                               >  2:280617/1‑107 (MQ=255)

                                                      cTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCA‑CCACACGCACgacgac                                                                                               <  1:280617/107‑1 (MQ=255)

                                                      cTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCA‑CCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGtt                                                               >  2:171913/1‑139 (MQ=255)

                                                           aCGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCA‑CCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCg                                                          <  1:1079173/139‑1 (MQ=255)

                                                             gAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGt                                                         >  2:1060356/1‑139 (MQ=255)

                                                              aaaaaCCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCA‑CCACACGCACGACGACCACTgcggc                                                                                      >  2:1195975/1‑108 (MQ=255)

                                                              aaaaaCCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCA‑CCACACGCACGACGACCACTgcggc                                                                                      <  1:1195975/108‑1 (MQ=255)

                                                              aaaaaCCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCA‑CCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTg                                                       >  2:35231/1‑139 (MQ=255)

                                                              aaaaaCCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCA‑CCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTg                                                       >  2:840578/1‑139 (MQ=255)

                                                                   ccccGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCA‑CCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCAt                                                  <  1:1566149/139‑1 (MQ=255)

                                                                        cccgGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCA‑CCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAag                                             <  1:840578/139‑1 (MQ=255)

                                                                             tttGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAagtagt                                         <  1:1060356/139‑1 (MQ=255)

                                                                                 cgcCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCA‑CCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAagtag                                          >  2:1253588/1‑133 (MQ=255)

                                                                                 cgcCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCA‑CCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAagtag                                          <  1:1253588/133‑1 (MQ=255)

                                                                                  gcagtcggGGTCTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCA‑CCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTgg                                   >  2:981989/6‑139 (MQ=255)

                                                                                                           ttgttCCAGTCCTTACGGCGCATTGCCGACGACCACCA‑CCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAAc          <  1:873042/139‑1 (MQ=255)

                                                                                                                   gTCCTTACGGCGCATTGCCGACGACCACCA‑CCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTta  >  2:739893/1‑139 (MQ=255)

CCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCA‑CCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTA  >  NC_000913/3852771‑3853024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A