Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	750	51.0	2595496	93.6%	2429384	106.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,852,890	C→T	33 (TAG→TAA)	ivbL ←	ilvB operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,852,890	0	C	T	100.0%	158.2 / NA	49	33 (TAG→TAA)	ivbL	ilvB operon leader peptide
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/29); total (20/29)

TGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTA  >  NC_000913/3852769‑3853024

tgCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCcgac                                                                                                                       <  2:826758/139‑1 (MQ=255)

tgCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCcgac                                                                                                                       >  2:757544/1‑139 (MQ=255)

   ccGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCgacgac                                                                                                                    <  1:78403/139‑1 (MQ=255)

   ccGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCgacgac                                                                                                                    <  1:771347/139‑1 (MQ=255)

   ccGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCgacgac                                                                                                                    <  2:232608/139‑1 (MQ=255)

      aaCTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGaccac                                                                                                                 <  1:281561/139‑1 (MQ=255)

         ttGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGaccaccac                                                                                                              <  1:14233/139‑1 (MQ=255)

                            ttcttcTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCAc                                                                                            >  2:1171729/1‑139 (MQ=255)

                            ttcttcTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACt                                                                                           >  2:26386/1‑139 (MQ=255)

                                 ctGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCcga                                                                                                                        >  1:1199337/1‑105 (MQ=255)

                                 ctGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCcga                                                                                                                        <  2:1199337/105‑1 (MQ=255)

                                    ggCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAA‑CGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGTGGCGGAt                                                                                  >  2:711184/1‑139 (MQ=255)

                                      ccGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATg                                                                                 <  1:26386/139‑1 (MQ=255)

                                         aCTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGgcg                                                                              >  1:12527/1‑139 (MQ=255)

                                         aCTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGgcg                                                                              >  2:706482/1‑139 (MQ=255)

                                              ccGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGtt                                                                         <  2:130336/139‑1 (MQ=255)

                                               cGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGCGGGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGtt                                                                         <  1:1171729/139‑1 (MQ=255)

                                                gggAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTgg                                                                       <  2:518410/139‑1 (MQ=255)

                                                     gTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGgtagta                                                                  >  2:23684/1‑139 (MQ=255)

                                                     gTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGgtagta                                                                  >  2:48255/1‑139 (MQ=255)

                                                       gCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGTGCAGTTGGTagtagt                                                                >  2:1234857/1‑139 (MQ=255)

                                                        cTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGtt                                                               >  2:86504/1‑139 (MQ=255)

                                                            aaCGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGc                                                           >  2:1014743/1‑139 (MQ=255)

                                                             aCGAAAAACCCCGCCTGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCg                                                          <  1:1053892/139‑1 (MQ=255)

                                                             aCGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCg                                                          <  2:1182376/139‑1 (MQ=255)

                                                             aCGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCg                                                          <  1:23684/139‑1 (MQ=255)

                                                             aCGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCg                                                          <  2:325605/139‑1 (MQ=255)

                                                               gAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGTGCAGTTGGTAGTAGTTTTGCGtt                                                        <  1:1234857/139‑1 (MQ=255)

                                                                  aaaCCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAg                                                     <  1:732112/139‑1 (MQ=255)

                                                                    aCCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTTCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCa                                                   <  2:866171/139‑1 (MQ=255)

                                                                    aCCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCa                                                   <  1:757544/139‑1 (MQ=255)

                                                                     ccccGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGc                                                                                                                           >  2:1136469/1‑66 (MQ=255)

                                                                     ccccGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGc                                                                                                                           <  1:1136469/66‑1 (MQ=255)

                                                                           ccgGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAagt                                            <  1:706482/139‑1 (MQ=255)

                                                                               tttGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTc                                        >  2:938878/1‑139 (MQ=255)

                                                                                 tGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCAt                                      <  1:938878/139‑1 (MQ=255)

                                                                                    gcCGGC‑GGGGTTTTGGAA‑CGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGTGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTggg                                  <  1:711184/139‑1 (MQ=255)

                                                                                              gTTTTGGAATCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCttgt                          >  2:1091250/1‑139 (MQ=255)

                                                                                                     aaTCGTGTGTTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGtttt                   <  1:1091250/139‑1 (MQ=255)

                                                                                                         gtgtgtTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGgcg                                                                              >  2:606190/1‑76 (MQ=255)

                                                                                                         gtgtgtTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGgcg                                                                              <  1:606190/76‑1 (MQ=255)

                                                                                                         gtgtgtTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTgtgt               >  2:9785/1‑139 (MQ=255)

                                                                                                           gtgtTGTTCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGttt             <  2:12527/139‑1 (MQ=255)

                                                                                                              ttgttCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAAc          <  1:86504/139‑1 (MQ=255)

                                                                                                               tgttCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACa         <  1:9785/139‑1 (MQ=255)

                                                                                                                 ttCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCa                                                   >  2:17392/1‑95 (MQ=255)

                                                                                                                 ttCCAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCa                                                   <  1:17392/95‑1 (MQ=255)

                                                                                                                   ccAGTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAatat     >  2:385068/1‑139 (MQ=255)

                                                                                                                      gTCCTTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTta  >  2:47301/1‑139 (MQ=255)

TGCCCGAACTTGCCATGCTCCAGTCCTTTTCTTCTGGGCCGACTTTCCGGGAGGTGCTTAAACGAAAAACCCCGCCCGGTTTGCGCCGGC‑GGGGTTTTGGAATCGTGTGTTGTTCCAGTCCCTACGGCGCATTGCCGACGACCACCACCACACGCACGACGACCACTGCGGCGGATGGCGCAGTTGGTAGTAGTTTTGCGTTGAGCATGGAAGTAGTCATTGGGGACCTTGTTGGTTTTGTGTTTAACAATATTTA  >  NC_000913/3852769‑3853024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A