Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A10 F1 I1 R1 | 759 | 65.2 | 3386260 | 90.7% | 3071337 | 101.6 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,965,557 | (T)8→7 | coding (74/1266 nt) | rhlB ← | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,965,550 | 0 | T | . | 100.0% | 190.2 / NA | 47 | coding (81/1266 nt) | rhlB | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (22/25); total (22/25) | |||||||||||
AGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGTG > NC_000913/3965416‑3965680 | agaagaTAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCttttt > 1:298946/1‑139 (MQ=255) aTGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAggg < 2:260500/139‑1 (MQ=255) aTGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAggg < 2:298946/139‑1 (MQ=255) aTGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAggg < 1:334740/139‑1 (MQ=255) tGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGc > 1:215154/1‑139 (MQ=255) ttGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACa > 2:1122733/1‑139 (MQ=255) aCGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTa > 1:1659701/1‑134 (MQ=255) aCGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTa < 2:1659701/134‑1 (MQ=255) gAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGa > 2:1106264/1‑139 (MQ=255) cGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCa > 1:105473/1‑139 (MQ=255) tCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCg < 2:1392516/139‑1 (MQ=255) cGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGa < 2:105473/139‑1 (MQ=255) cGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGaa < 2:215154/139‑1 (MQ=255) gTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctg > 1:160528/1‑139 (MQ=255) tttGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgt < 2:712374/139‑1 (MQ=255) cgcCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCtttttttcaag < 2:1442243/89‑5 (MQ=255) cgcCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCtttttttcaag > 1:1442243/1‑85 (MQ=255) cgcCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCt > 1:119881/1‑96 (MQ=255) cgcCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCt < 2:119881/96‑1 (MQ=255) cgcCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCg > 1:250106/1‑106 (MQ=255) cgcCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCg < 2:250106/106‑1 (MQ=255) gcCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTtctgttctgt < 1:1122733/139‑1 (MQ=255) cccGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACtt < 2:1339904/125‑1 (MQ=255) cccGGCTACGTCACGACCCCCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACtt > 1:1339904/1‑125 (MQ=255) tCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCt > 1:1370639/1‑139 (MQ=255) aCGACCCGCCGGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCa < 2:702252/139‑1 (MQ=255) cGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCa < 1:437389/133‑1 (MQ=255) cGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCa > 2:437389/1‑133 (MQ=255) cGCCAGCGTCACCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAgtgt < 1:1106264/139‑1 (MQ=255) gCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTa < 2:428590/72‑1 (MQ=255) gCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTa > 1:428590/1‑72 (MQ=255) aGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAggg < 1:1632129/57‑1 (MQ=255) aGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAggg > 2:1632129/1‑57 (MQ=255) aGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGt > 2:138567/1‑81 (MQ=255) aGGGACAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGt < 1:138567/81‑1 (MQ=255) gggCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTa > 1:889949/1‑139 (MQ=255) gggCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTa > 2:219818/1‑139 (MQ=255) gCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTa < 2:1329468/48‑1 (MQ=255) gCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTa > 1:1329468/1‑48 (MQ=255) aaTGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACg < 2:160528/139‑1 (MQ=255) gTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATa < 2:839559/98‑1 (MQ=255) gTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATa > 1:839559/1‑98 (MQ=255) aCAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCtt < 1:563673/41‑1 (MQ=255) aCAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCtt > 2:563673/1‑41 (MQ=255) cAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATc < 1:458377/139‑1 (MQ=255) aTGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATg < 2:1022446/41‑1 (MQ=255) aTGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATg > 1:1022446/1‑41 (MQ=255) aTGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACtt < 2:1684960/62‑1 (MQ=255) aTGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACtt > 1:1684960/1‑62 (MQ=255) aTGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGTg > 1:1094352/1‑139 (MQ=255) | AGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGTG > NC_000913/3965416‑3965680 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |