Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I1 R1 750 51.0 2595496 93.6% 2429384 106.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,507,942 A→G Y217H (TAC→CAC)  php ← phosphotriesterase homology protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,507,9420AG100.0% 228.4 / NA 65Y217H (TAC→CAC) phpphosphotriesterase homology protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/30);  total (35/30)

AGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTGG  >  NC_000913/3507813‑3508069
                                                                                                                                  |                                                                                                                               
aGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGtt                                                                                                                        >  2:699192/1‑139 (MQ=255)
  tCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCt                                                                                                                      >  1:1254567/1‑139 (MQ=255)
    aTAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTg                                                                                                                    >  1:328024/1‑139 (MQ=255)
     tAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGc                                                                                                                   >  1:916859/1‑139 (MQ=255)
        ccATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGa                                                                                                                <  1:1295329/139‑1 (MQ=255)
            aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGgt                                                                                                            >  1:1137453/1‑139 (MQ=255)
            aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGgt                                                                                                            >  2:910325/1‑139 (MQ=255)
            aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGgt                                                                                                            >  2:706252/1‑139 (MQ=255)
             accaccGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGgtg                                                                                                           <  1:706252/139‑1 (MQ=255)
                accGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCg                                                                                                        <  2:328024/139‑1 (MQ=255)
                accGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCCATGGTGTCg                                                                                                        >  1:832160/1‑139 (MQ=255)
                  cGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATg                                                                                                              >  1:510383/1‑131 (MQ=255)
                  cGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATg                                                                                                              <  2:510383/131‑1 (MQ=255)
                  cGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGaa                                                                                                      >  1:239429/1‑139 (MQ=255)
                    ttGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACt                                                                                                    >  1:16935/1‑139 (MQ=255)
                       gCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGGCGATGGTGTCGAACTGCa                                                                                                 >  1:871233/1‑139 (MQ=255)
                       gCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCa                                                                                                 <  2:239429/139‑1 (MQ=255)
                         ttttAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACg                                                                                               <  2:1254567/139‑1 (MQ=255)
                           ttAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                             <  2:452387/139‑1 (MQ=255)
                           ttAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                             <  2:1291745/139‑1 (MQ=255)
                                tGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACgcgc                                                                                        <  2:16935/139‑1 (MQ=255)
                                 gggAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGcc                                                                                       >  2:664987/1‑139 (MQ=255)
                                   gAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCga                                                                                     >  2:496734/1‑139 (MQ=255)
                                      cggcgCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgat                                                                                  <  1:699192/139‑1 (MQ=255)
                                         cgcgTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGa                                                                                                                <  1:205891/106‑1 (MQ=255)
                                         cgcgTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGa                                                                                                                >  2:205891/1‑106 (MQ=255)
                                           cgtaagagtcATCGAGAGCATGACCCGGTTCAGCAACACACTGTCGCGGAGCACATGAAAGCA‑CCCAATACGCTTTTCGTACCGGGGCTAACTGTTCTTGCCGATAGTGTCAAGATTCACGTACGCGCCGAgatcgagc                                                                             <  2:776136/130‑3 (MQ=255)
                                           cgTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgatcgatc                                                                             <  1:496734/139‑1 (MQ=255)
                                                     aTCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTaa                                                                                                                             <  2:713814/81‑1 (MQ=255)
                                                     aTCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTaa                                                                                                                             >  1:713814/1‑81 (MQ=255)
                                                                gACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAgg                                                        <  1:664987/139‑1 (MQ=255)
                                                                gACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAgg                                                        >  2:780590/1‑139 (MQ=255)
                                                                  cgcgGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGtt                                                      <  1:910325/139‑1 (MQ=255)
                                                                  cgcgGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGtt                                                      >  1:446331/1‑139 (MQ=255)
                                                                       ttCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                             <  2:1082978/95‑1 (MQ=255)
                                                                       ttCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                             >  1:1082978/1‑95 (MQ=255)
                                                                            cAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGa                                            <  2:871233/139‑1 (MQ=255)
                                                                               cccACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCg                                         >  1:309580/1‑139 (MQ=255)
                                                                                 cACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCa                                       <  1:155293/139‑1 (MQ=255)
                                                                                    ggTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTTGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAgg                                                        <  1:260213/119‑1 (MQ=255)
                                                                                    ggTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTTGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAgg                                                        >  2:260213/1‑119 (MQ=255)
                                                                                         cgTAGCGCATG‑AAGCATCGTAATACGCTTTTCGTCCGTGTGGTAACTGTTCTTGCCGATGGTGTCGaa                                                                                                      <  2:1274807/68‑1 (MQ=255)
                                                                                         cgTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGaa                                                                                                      >  1:1274807/1‑68 (MQ=255)
                                                                                         cgTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                             <  2:402164/77‑1 (MQ=255)
                                                                                         cgTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                             >  1:402164/1‑77 (MQ=255)
                                                                                         cgTAGCGCATG‑AAGCATCGCAATACGCTTTTCGACCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAATTGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCaa                               >  1:283582/1‑139 (MQ=255)
                                                                                           tAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACg                             >  1:1118913/1‑139 (MQ=255)
                                                                                                cATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGAc                        >  1:154250/1‑139 (MQ=255)
                                                                                                 aTG‑AAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGAcg                       <  1:538821/139‑1 (MQ=255)
                                                                                                     aaGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGGTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGAcgcgc                    <  2:916859/139‑1 (MQ=255)
                                                                                                       gCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGa                  <  2:309580/139‑1 (MQ=255)
                                                                                                          tCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                             <  1:347201/61‑1 (MQ=255)
                                                                                                          tCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                             >  2:347201/1‑61 (MQ=255)
                                                                                                          tCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAg               >  2:613469/1‑139 (MQ=255)
                                                                                                                tACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCg                                                                                                        <  2:159236/44‑1 (MQ=255)
                                                                                                                tACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCg                                                                                                        >  1:159236/1‑44 (MQ=255)
                                                                                                                tACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCtt                                                 <  2:459672/99‑1 (MQ=255)
                                                                                                                tACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCtt                                                 >  1:459672/1‑99 (MQ=255)
                                                                                                                tACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAAc         >  1:511104/1‑139 (MQ=255)
                                                                                                                     ttttCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgatcgatc                                                                             >  2:1229815/1‑66 (MQ=255)
                                                                                                                     ttttCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgatcgatc                                                                             <  1:1229815/66‑1 (MQ=255)
                                                                                                                     ttttCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAgg                                                        <  2:505985/87‑1 (MQ=255)
                                                                                                                     ttttCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAgg                                                        >  1:505985/1‑87 (MQ=255)
                                                                                                                       ttCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgat                                                                                  <  1:827934/59‑1 (MQ=255)
                                                                                                                       ttCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgat                                                                                  >  2:827934/1‑59 (MQ=255)
                                                                                                                       ttCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTgg  >  1:924120/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                               
AGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATG‑AAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTGG  >  NC_000913/3507813‑3508069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A