Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I2 R1 765 53.2 2691310 93.4% 2513683 103.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,507,942 A→G Y217H (TAC→CAC)  php ← phosphotriesterase homology protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,507,9420AG98.2% 190.4 / ‑3.3 55coding (649/879 nt)phpphosphotriesterase homology protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (28/26);  minor base . (1/0);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

TAAATAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTGGGCTTG  >  NC_000913/3507808‑3508074
                                                                                                                                      |                                                                                                                                    
tAAATAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTaa                                                                                                                                  >  1:770151/1‑139 (MQ=255)
       tCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCt                                                                                                                           >  2:340357/1‑139 (MQ=255)
          tAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGc                                                                                                                        >  2:649657/1‑139 (MQ=255)
             ccATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGa                                                                                                                     <  2:660929/139‑1 (MQ=255)
                tAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATgg                                                                                                                  >  1:1215844/1‑139 (MQ=255)
                 aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGgt                                                                                                                 >  1:470936/1‑139 (MQ=255)
                   ccaccGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGgtgt                                                                                                               <  1:160743/139‑1 (MQ=255)
                      ccGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCg                                                                                                             <  2:858133/138‑1 (MQ=255)
                      ccGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCg                                                                                                             >  1:858133/1‑138 (MQ=255)
                      ccGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGa                                                                                                            >  1:399954/1‑139 (MQ=255)
                          tGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTg                                                                                                        <  1:649657/139‑1 (MQ=255)
                           ggCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGgtggta                                                                                                                                   >  1:1117871/1‑111 (MQ=255)
                           ggCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGgtggta                                                                                                                                   <  2:1117871/111‑1 (MQ=255)
                           ggCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGa                                                                                                                     <  2:733237/125‑1 (MQ=255)
                           ggCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGa                                                                                                                     >  1:733237/1‑125 (MQ=255)
                            gCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAATTGCa                                                                                                      <  1:340357/139‑1 (MQ=255)
                                ttAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                                  <  2:519546/139‑1 (MQ=255)
                                ttAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                                  <  2:770151/139‑1 (MQ=255)
                                     tGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACgcgc                                                                                             <  2:470936/139‑1 (MQ=255)
                                           cggcgCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgat                                                                                       >  2:450604/1‑139 (MQ=255)
                                             gcgcGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgatcg                                                                                     <  2:399954/139‑1 (MQ=255)
                                                  tAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTACACGTACGCGCCGAGATCGatcat                                                                                <  1:1047210/139‑1 (MQ=255)
                                                          aTCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGACGAGATCGATCATCTTCaaaa                                                                        >  1:792125/1‑139 (MQ=255)
                                                           tCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAt                                                                       >  1:215793/1‑139 (MQ=255)
                                                            cGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCaaa                                                                         <  1:907356/136‑1 (MQ=255)
                                                            cGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCaaa                                                                         >  2:907356/1‑136 (MQ=255)
                                                                 gCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                                  >  2:528625/1‑106 (MQ=255)
                                                                 gCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                                  <  1:528625/106‑1 (MQ=255)
                                                                 gCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTc                                                                 >  1:1195496/1‑139 (MQ=255)
                                                                  cATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTaa                                                                                                                                  <  1:1123605/73‑1 (MQ=255)
                                                                  cATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTaa                                                                                                                                  >  2:1123605/1‑73 (MQ=255)
                                                                  cATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCaaaa                                                                        >  1:968428/1‑131 (MQ=255)
                                                                  cATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCaaaa                                                                        <  2:968428/131‑1 (MQ=255)
                                                                    tGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTaa                                                                                                                                  <  1:167389/71‑1 (MQ=255)
                                                                    tGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTaa                                                                                                                                  >  2:167389/1‑71 (MQ=255)
                                                                     gACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAgg                                                             <  2:792125/139‑1 (MQ=255)
                                                                       cgcgGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGtt                                                           <  1:527018/139‑1 (MQ=255)
                                                                       cgcgGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGtt                                                           >  2:90875/1‑139 (MQ=255)
                                                                            ttCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGatcat                                                                                >  1:519767/1‑113 (MQ=255)
                                                                            ttCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGatcat                                                                                <  2:519767/113‑1 (MQ=255)
                                                                                 cAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGa                                                 >  1:57850/1‑139 (MQ=255)
                                                                                      cACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCaaaa                                                                        <  1:360314/111‑1 (MQ=255)
                                                                                      cACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCaaaa                                                                        >  2:360314/1‑111 (MQ=255)
                                                                                              cgTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCaa                                    <  2:57850/139‑1 (MQ=255)
                                                                                              cgTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCaa                                    >  1:855825/1‑139 (MQ=255)
                                                                                                             aTCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGtt                                                           >  2:339018/1‑101 (MQ=255)
                                                                                                             aTCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGtt                                                           <  1:339018/101‑1 (MQ=255)
                                                                                                             aTCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGa                                                 >  2:121323/1‑111 (MQ=255)
                                                                                                             aTCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGa                                                 <  1:121323/111‑1 (MQ=255)
                                                                                                              tCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAg                    >  1:711912/1‑139 (MQ=255)
                                                                                                              tCGCAATACGCTTTTCGTCCGG‑‑‑GTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATc                 >  1:164769/1‑139 (MQ=255)
                                                                                                                  aaTACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCga                                                                                          >  2:418217/1‑65 (MQ=255)
                                                                                                                  aaTACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCga                                                                                          <  1:418217/65‑1 (MQ=255)
                                                                                                                    tACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGa                                                                                                                     <  1:853997/36‑1 (MQ=255)
                                                                                                                    tACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGa                                                                                                                     >  2:853997/1‑36 (MQ=255)
                                                                                                                         ttttCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCa                                            <  2:1338285/104‑1 (MQ=255)
                                                                                                                         ttttCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCa                                            >  1:1338285/1‑104 (MQ=255)
                                                                                                                                ccGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTGGGCTTg  >  1:1205182/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                    
TAAATAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTGGGCTTG  >  NC_000913/3507808‑3508074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A