Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I2 R1
|
774 |
49.2 |
2112398 |
96.9% |
2046913 |
118.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,773,602 |
T→C |
V441A (GTT→GCT) |
mtlA → |
mannitol‑specific PTS enzyme: IIA, IIB and IIC components |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,773,602 | 0 | T | C | 100.0%
| 131.2
/ NA
| 37 | V441A (GTT→GCT) | mtlA | mannitol‑specific PTS enzyme: IIA, IIB and IIC components |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/20); total (17/20) |
TAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTT > NC_000913/3773477‑3773737
|
tAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAg > 1:1010436/1‑139 (MQ=255)
cAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACt < 2:25417/130‑1 (MQ=255)
cAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACt > 1:25417/1‑130 (MQ=255)
cAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACa < 2:129483/139‑1 (MQ=255)
cAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACa < 2:1010436/139‑1 (MQ=255)
gATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGc < 1:243183/139‑1 (MQ=255)
ttCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGc < 1:276980/139‑1 (MQ=255)
aaCAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCa > 1:516695/1‑139 (MQ=255)
cgcgATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTg > 2:1043909/1‑139 (MQ=255)
cgATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGAc > 2:34315/1‑139 (MQ=255)
aTCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCg > 1:385103/1‑139 (MQ=255)
aTCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCg > 2:642182/1‑139 (MQ=255)
tCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGa < 2:832154/139‑1 (MQ=255)
acaacCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACg < 1:922688/139‑1 (MQ=255)
cGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACg > 2:919314/1‑139 (MQ=255)
gTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCaa < 1:419305/129‑1 (MQ=255)
gTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCaa > 2:419305/1‑129 (MQ=255)
gTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACgg > 2:657439/1‑139 (MQ=255)
gTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACgg < 1:348130/139‑1 (MQ=255)
catcaCTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCa < 2:298738/139‑1 (MQ=255)
ccGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGc < 1:110890/91‑1 (MQ=255)
ccGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGc > 2:110890/1‑91 (MQ=255)
cGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAgaga < 2:575508/139‑1 (MQ=255)
cGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAgaga < 2:618120/139‑1 (MQ=255)
gTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGaa > 1:427567/1‑139 (MQ=255)
acctgaccGAACGCGCTATGCGCCAGGCTCCGCAgg > 1:637182/1‑36 (MQ=255)
acctgaccGAACGCGCTATGCGCCAGGCTCCGCAgg < 2:637182/36‑1 (MQ=255)
accGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTaaaa > 2:261837/1‑138 (MQ=255)
accGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTaaaa < 1:261837/138‑1 (MQ=255)
ccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAgaga < 2:243990/129‑1 (MQ=255)
ccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAgaga > 1:243990/1‑129 (MQ=255)
aCGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGc > 1:55124/1‑64 (MQ=255)
aCGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGc < 2:55124/64‑1 (MQ=255)
cgcCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTac > 1:941555/1‑60 (MQ=255)
cgcCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTac < 2:941555/60‑1 (MQ=255)
cAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCt < 1:34315/139‑1 (MQ=255)
aGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCtt < 2:29633/139‑1 (MQ=255)
|
TAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCTT > NC_000913/3773477‑3773737
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
