Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	757	76.1	3598070	93.4%	3360597	108.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,773,602	T→C	V441A (GTT→GCT)	mtlA →	mannitol‑specific PTS enzyme: IIA, IIB and IIC components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,773,602	0	T	C	100.0%	170.6 / NA	47	V441A (GTT→GCT)	mtlA	mannitol‑specific PTS enzyme: IIA, IIB and IIC components
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/24); total (23/24)

TTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCT  >  NC_000913/3773470‑3773736

ttCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCa                                                                                                                                  >  2:1225072/1‑139 (MQ=255)

ttCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCa                                                                                                                                  <  1:857698/139‑1 (MQ=255)

      gTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGAAGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGcaca                                                                                                                            <  1:1225072/139‑1 (MQ=255)

         aGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGCCCGAACGCGCTATGCGCCAGGCTCCGc                                                                                                                                   <  1:1767732/129‑1 (MQ=255)

         aGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGc                                                                                                                                   >  2:1767732/1‑129 (MQ=255)

              aTTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATAttt                                                                                                                    <  2:838878/139‑1 (MQ=255)

                              tGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATA‑TTCGCTGACCAACTTCCTc                                                                                                   >  2:891382/1‑139 (MQ=255)

                                tCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCg                                                                                                  >  1:1173880/1‑139 (MQ=255)

                                tCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCg                                                                                                  >  1:913230/1‑139 (MQ=255)

                                      aTTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCg                                                                                            <  2:976024/139‑1 (MQ=255)

                                      aTTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCg                                                                                            <  2:501115/139‑1 (MQ=255)

                                        ttCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGc                                                                                          <  1:1216692/139‑1 (MQ=255)

                                          cTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCCCT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCt                                                                                        <  2:590280/139‑1 (MQ=255)

                                                  aaCAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCg                                                                                            <  1:1060991/127‑1 (MQ=255)

                                                  aaCAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCg                                                                                            >  2:1060991/1‑127 (MQ=255)

                                                     aGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGcc                                                                             >  1:2761/1‑139 (MQ=255)

                                                       cgcgATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGcc                                                                             >  2:389929/1‑139 (MQ=255)

                                                       cgcgATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTg                                                                           >  2:1510195/1‑139 (MQ=255)

                                                           aTCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGAc                                                                         <  1:389929/139‑1 (MQ=255)

                                                                 aacCTGCGGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGaa                                                                     <  2:1772938/135‑1 (MQ=255)

                                                                 aacCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGaa                                                                     >  1:1772938/1‑135 (MQ=255)

                                                                 aacCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTc                                                                 <  2:1173880/139‑1 (MQ=255)

                                                                      gccgccAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGtt                                                            >  1:32720/1‑139 (MQ=255)

                                                                        cgccAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGc                                                                                             <  1:959208/104‑1 (MQ=255)

                                                                        cgccAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGc                                                                                             >  2:959208/1‑104 (MQ=255)

                                                                        cgccAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTtgc                                                          >  1:306980/1‑139 (MQ=255)

                                                                              aTGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCa                                                    >  2:888267/1‑139 (MQ=255)

                                                                                        cGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCg                                                                                                                                    <  1:221796/49‑1 (MQ=255)

                                                                                        cGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCg                                                                                                                                    >  2:221796/1‑49 (MQ=255)

                                                                                         gTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACgg                                         <  2:913230/139‑1 (MQ=255)

                                                                                         gTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACgg                                         >  1:149789/1‑139 (MQ=255)

                                                                                         gTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACgg                                         >  2:470352/1‑139 (MQ=255)

                                                                                         gTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACgg                                         >  1:949824/1‑139 (MQ=255)

                                                                                                    cacCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATA‑TTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAaga                               <  1:891382/139‑1 (MQ=255)

                                                                                                     acCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAaga                               <  1:554/139‑1 (MQ=255)

                                                                                                       cGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAgaga                             <  2:306980/139‑1 (MQ=255)

                                                                                                         tgacctgaccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGaaa                           >  2:960062/1‑139 (MQ=255)

                                                                                                               gaccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAaga                               >  2:387703/1‑129 (MQ=255)

                                                                                                               gaccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAaga                               <  1:387703/129‑1 (MQ=255)

                                                                                                                 ccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCg                                                                                            <  1:1318578/66‑1 (MQ=255)

                                                                                                                 ccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCg                                                                                            >  2:1318578/1‑66 (MQ=255)

                                                                                                                 ccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGa                   <  2:149789/139‑1 (MQ=255)

                                                                                                                   gAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACa                 <  1:333228/139‑1 (MQ=255)

                                                                                                                     aCGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGc               >  2:1003223/1‑139 (MQ=255)

                                                                                                                            aTGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAgaga                             >  2:74346/1‑118 (MQ=255)

                                                                                                                            aTGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAgaga                             <  1:74346/118‑1 (MQ=255)

                                                                                                                                  cAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCt  <  2:949824/139‑1 (MQ=255)

TTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCT  >  NC_000913/3773470‑3773736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A