Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	765	53.2	2691310	93.4%	2513683	103.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,897,439	G→A	222 (TAG→TAA)	yieH →	phosphoenolpyruvate and 6‑phosphogluconate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,897,439	0	G	A	100.0%	283.2 / NA	89	222 (TAG→TAA)	yieH	phosphoenolpyruvate and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (46/43); total (46/43)

GAAGTGTTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAGTTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGT  >  NC_000913/3897314‑3897573

gAAGTGTTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTcc                                                                                                                           >  2:690862/1‑139 (MQ=255)

 aaGTGTTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTccc                                                                                                                          <  1:690862/139‑1 (MQ=255)

     gtTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCa                                                                                                                      >  1:974818/1‑139 (MQ=255)

     gtTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCa                                                                                                                      >  1:1188231/1‑139 (MQ=255)

      ttCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCAc                                                                                                                     >  2:257105/1‑139 (MQ=255)

      ttCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCAc                                                                                                                     >  1:40166/1‑139 (MQ=255)

       tCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACt                                                                                                                    <  1:240407/139‑1 (MQ=255)

           cTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTAcc                                                                                                                <  2:931384/139‑1 (MQ=255)

              cTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCg                                                                                                             >  2:1305352/1‑139 (MQ=255)

                gcgcCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCt                                                                                                           >  2:320479/1‑139 (MQ=255)

                gcgcCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCt                                                                                                           >  1:146115/1‑139 (MQ=255)

                   ccGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTaa                                                                                                        >  2:575314/1‑139 (MQ=255)

                      aCCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCacac                                                                                                                              >  2:639408/1‑114 (MQ=255)

                      aCCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCacac                                                                                                                              <  1:639408/114‑1 (MQ=255)

                      aCCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTAc                                                                                                                 >  1:617149/1‑127 (MQ=255)

                      aCCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTAc                                                                                                                 <  2:617149/127‑1 (MQ=255)

                      aCCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAAtt                                                                                                     >  1:494792/1‑139 (MQ=255)

                      aCCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAAtt                                                                                                     >  1:10286/1‑139 (MQ=255)

                            acaATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCt                                                                                               <  2:1188231/139‑1 (MQ=255)

                            acaATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCt                                                                                               <  2:417765/139‑1 (MQ=255)

                             caATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTc                                                                                              <  2:10286/139‑1 (MQ=255)

                              aaTAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCa                                                                                             <  2:146115/139‑1 (MQ=255)

                                  aGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAgg                                                                                         <  1:257105/139‑1 (MQ=255)

                                  aGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAgg                                                                                         <  1:213053/139‑1 (MQ=255)

                                  aGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAgg                                                                                         <  1:1305352/139‑1 (MQ=255)

                                        tCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGgtaagta                                                                                   >  2:472296/1‑139 (MQ=255)

                                        tCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGgtaagta                                                                                   >  1:1041626/1‑139 (MQ=255)

                                        tCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGgtaagta                                                                                   >  1:160936/1‑139 (MQ=255)

                                            tCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCt                                                                                               <  2:249112/123‑1 (MQ=255)

                                            tCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCt                                                                                               >  1:249112/1‑123 (MQ=255)

                                              aCCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGa                                                                             >  1:1161824/1‑139 (MQ=255)

                                                   aaaGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTccc                                                                                                                          >  2:362063/1‑89 (MQ=255)

                                                   aaaGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTccc                                                                                                                          <  1:362063/89‑1 (MQ=255)

                                                         accaccTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGt                                                                  <  1:320479/139‑1 (MQ=255)

                                                              cTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGtt                                                             <  2:40166/139‑1 (MQ=255)

                                                                ttACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAggg                                                                              <  2:780760/120‑1 (MQ=255)

                                                                ttACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAggg                                                                              >  1:780760/1‑120 (MQ=255)

                                                                   cccATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTTACg                                                        <  2:1161824/139‑1 (MQ=255)

                                                                   cccATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACg                                                        <  2:1041626/139‑1 (MQ=255)

                                                                        cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAAttctt                                                                                                                                   <  1:953304/59‑1 (MQ=255)

                                                                        cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAAttc                                                                                                                                     >  1:625671/1‑57 (MQ=255)

                                                                        cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAAttc                                                                                                                                     <  2:625671/57‑1 (MQ=255)

                                                                        cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTcaca                                                                                                                               >  2:287233/1‑63 (MQ=255)

                                                                        cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTcaca                                                                                                                               <  1:659458/63‑1 (MQ=255)

                                                                        cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTcaca                                                                                                                               <  1:287233/63‑1 (MQ=255)

                                                                        cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTcaca                                                                                                                               >  2:659458/1‑63 (MQ=255)

                                                                        cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAg                                                                                >  2:1167175/1‑110 (MQ=255)

                                                                        cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAg                                                                                <  1:1167175/110‑1 (MQ=255)

                                                                        cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCggg                                                   >  2:1197974/1‑139 (MQ=255)

                                                                        cTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATGACGGCATAAttctt                                                                                                                                   >  2:953304/1‑59 (MQ=255)

                                                                           tCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTaa                                                <  2:232294/139‑1 (MQ=255)

                                                                            cGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAAt                                               <  2:494792/139‑1 (MQ=255)

                                                                                 ttACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGa                                                                       <  1:893077/110‑1 (MQ=255)

                                                                                 ttACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGa                                                                       >  2:893077/1‑110 (MQ=255)

                                                                                        aaCTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACt                                   >  2:233194/1‑139 (MQ=255)

                                                                                         aCTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACg                                                        >  2:1234983/1‑117 (MQ=255)

                                                                                         aCTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACg                                                        <  1:1234983/117‑1 (MQ=255)

                                                                                             tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTgg                                                                                                   >  2:1341068/1‑70 (MQ=255)

                                                                                             tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTgg                                                                                                   <  1:1341068/70‑1 (MQ=255)

                                                                                             tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGttt                                                            <  2:1119732/109‑1 (MQ=255)

                                                                                             tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGttt                                                            >  1:1119732/1‑109 (MQ=255)

                                                                                             tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTc                                                      <  2:730453/115‑1 (MQ=255)

                                                                                             tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTc                                                      >  1:730453/1‑115 (MQ=255)

                                                                                             tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTAtt                              >  2:981037/1‑139 (MQ=255)

                                                                                             tgGAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTAtt                              >  2:1290195/1‑139 (MQ=255)

                                                                                               gAAAGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTAtttt                            <  2:160936/139‑1 (MQ=255)

                                                                                                 aaGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGc                          <  1:472296/139‑1 (MQ=255)

                                                                                                 aaGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGc                          <  1:575314/139‑1 (MQ=255)

                                                                                                 aaGCGCGTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGc                          <  1:1197974/139‑1 (MQ=255)

                                                                                                       gTGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGtttt                    >  1:346685/1‑139 (MQ=255)

                                                                                                       atGGTTGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGtttt                    >  1:458240/2‑139 (MQ=255)

                                                                                                           ttGGGATATTACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGa                <  2:346685/139‑1 (MQ=255)

                                                                                                                    tACGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCTCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAAtt       >  1:631830/1‑139 (MQ=255)

                                                                                                                     aCGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTaaa                                                                                                       >  1:48488/1‑42 (MQ=255)

                                                                                                                     aCGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTaaa                                                                                                       <  2:48488/42‑1 (MQ=255)

                                                                                                                     aCGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAg                                                                                >  1:557579/1‑65 (MQ=255)

                                                                                                                     aCGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAg                                                                                <  2:557579/65‑1 (MQ=255)

                                                                                                                     aCGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACg                                                        <  2:1071080/89‑1 (MQ=255)

                                                                                                                     aCGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACg                                                        >  1:1071080/1‑89 (MQ=255)

                                                                                                                     aCGGCATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTg      <  1:1290195/139‑1 (MQ=255)

                                                                                                                         cATAATTCTTCACACTCCCTTCACTTACTCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGgtgt  >  1:834445/1‑139 (MQ=255)

                                                                                                                         cATAATTCTTCACACTCCCTTCACTTACCCGGCTTAAATTGGCGCTCAAAGGTAGGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAGTACATCACGGACTTTATTTGGCAGTTTTAGGACAGTGAATTGgtgg  >  2:150239/1‑138 (MQ=255)

                                                                                                                         cATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGgta                                                                                       <  2:652926/54‑1 (MQ=255)

                                                                                                                         cATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGgta                                                                                       >  1:652926/1‑54 (MQ=255)

                                                                                                                         cATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGtt                                                                          <  1:1082459/67‑1 (MQ=255)

                                                                                                                         cATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGtt                                                                          >  2:1082459/1‑67 (MQ=255)

                                                                                                                         cATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACg                                                        <  2:1057301/85‑1 (MQ=255)

                                                                                                                         cATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACg                                                        >  1:1057301/1‑85 (MQ=255)

                                                                                                                         cATAATTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGgtgt  >  2:713033/1‑139 (MQ=255)

GAAGTGTTCTACTTCTGCGCCGACCCGCACAATAAGCCGATCGTTCACCCGAAAGTCACCACCTTTACCCATCTTTCGCAGTTACCTGAACTGTGGAAAGCGCGTGGTTGGGATATTACGGCATAGTTCTTCACACTCCCTTCACTTACCCCGCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGT  >  NC_000913/3897314‑3897573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A