Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I2 R1	760	52.7	2744488	90.2%	2475528	105.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,992,054	C→T	P301L (CCA→CTA)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,992,054	0	C	T	100.0%	150.4 / NA	47	P301L (CCA→CTA)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (25/22); total (25/22)

CCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGTAGGC  >  NC_000913/3991924‑3992187

ccATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                               >  2:1352234/1‑139 (MQ=255)

ccATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGACATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                               <  1:1203545/139‑1 (MQ=255)

                    aaCACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACt                                                                                                           <  2:777036/139‑1 (MQ=255)

                    aaCACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACt                                                                                                           <  2:569484/139‑1 (MQ=255)

                                 gAAGCCTATTCCTGGGAATGCCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACgg                                                                                              >  2:913156/1‑139 (MQ=255)

                                        aTTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGa                                                                                       >  1:1181006/1‑139 (MQ=255)

                                               ggAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCgtgt                                                                                                               >  1:166346/1‑108 (MQ=255)

                                               ggAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGAtttt                                                                                >  1:134616/1‑139 (MQ=255)

                                               ggAATACCCGAACCCACGTCGGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCgtgt                                                                                                               <  2:166346/108‑1 (MQ=255)

                                                   tACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCtata                                                                                                                                    <  2:975189/83‑1 (MQ=255)

                                                   tACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCtata                                                                                                                                    >  1:975189/1‑83 (MQ=255)

                                                   tACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTAccc                                                                            >  2:937613/1‑139 (MQ=255)

                                                      ccGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCCAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTc                                                                         <  1:913156/139‑1 (MQ=255)

                                                              aCGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTa                                                                 >  2:732643/1‑139 (MQ=255)

                                                                     ctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTAc                                                             >  1:344495/1‑136 (MQ=255)

                                                                     ctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTAc                                                             <  2:344495/136‑1 (MQ=255)

                                                                     ctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTc                                                          >  1:928439/1‑139 (MQ=255)

                                                                            aaGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                                   <  1:1094627/139‑1 (MQ=255)

                                                                            aaGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                                   <  2:809643/139‑1 (MQ=255)

                                                                            aaGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                                   <  1:937613/139‑1 (MQ=255)

                                                                            aaGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                                   <  1:781263/139‑1 (MQ=255)

                                                                                  tCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTa                                                                                                             >  2:514551/1‑75 (MQ=255)

                                                                                  tCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTa                                                                                                             <  1:514551/75‑1 (MQ=255)

                                                                                   cAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTa                                            <  1:732643/139‑1 (MQ=255)

                                                                                   cAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTa                                            >  1:1271514/1‑139 (MQ=255)

                                                                                     aaCAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTaaa                                          >  1:1315/1‑139 (MQ=255)

                                                                                      aCAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                                   <  2:1246758/129‑1 (MQ=255)

                                                                                      aCAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                                   >  1:1246758/1‑129 (MQ=255)

                                                                                         gCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGt                                                                          <  2:40293/103‑1 (MQ=255)

                                                                                         gCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGt                                                                          >  1:40293/1‑103 (MQ=255)

                                                                                          cGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCatga                                                                                                                            <  2:68970/52‑1 (MQ=255)

                                                                                          cGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCatga                                                                                                                            >  1:68970/1‑52 (MQ=255)

                                                                                          cGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTGTTTAAAAgtgt                                     >  2:892658/1‑139 (MQ=255)

                                                                                          cGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAgtgt                                     >  2:4078/1‑139 (MQ=255)

                                                                                            tttGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGc                                   >  2:614584/1‑139 (MQ=255)

                                                                                                    acgGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCg                                                                           >  1:861338/1‑91 (MQ=255)

                                                                                                    acgGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCg                                                                           <  2:861338/91‑1 (MQ=255)

                                                                                                    acgGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCt                           >  2:1272352/1‑139 (MQ=255)

                                                                                                    acgGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACACGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCt                           >  1:1306636/1‑139 (MQ=255)

                                                                                                        cGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGc                       >  1:1351140/1‑139 (MQ=255)

                                                                                                           gaTTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGt                    <  2:134616/139‑1 (MQ=255)

                                                                                                              tGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGaa                 <  2:1306636/139‑1 (MQ=255)

                                                                                                                tATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAAcg               >  2:833626/1‑139 (MQ=255)

                                                                                                                  tCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAAcgcg             <  1:833626/139‑1 (MQ=255)

                                                                                                                   cGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACgcgc            <  1:1272352/139‑1 (MQ=255)

                                                                                                                          tctcGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTc                                                                         >  2:1240459/1‑71 (MQ=255)

                                                                                                                          tctcGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTc                                                                         <  1:1240459/71‑1 (MQ=255)

                                                                                                                          tctcGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGTa     >  1:278333/1‑139 (MQ=255)

                                                                                                                             cGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGTAGGc  <  1:892658/139‑1 (MQ=255)

CCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCGTAGGC  >  NC_000913/3991924‑3992187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A