Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,992,054	C→T	P301L (CCA→CTA)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,992,054	0	C	T	100.0%	160.2 / NA	50	P301L (CCA→CTA)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/28); total (22/28)

TCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCG  >  NC_000913/3991921‑3992182

tCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACt                                                                                                                             >  1:117030/1‑139 (MQ=255)

   ccATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                          <  1:740264/139‑1 (MQ=255)

   ccATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                          <  2:154412/139‑1 (MQ=255)

   ccATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                          <  2:445905/139‑1 (MQ=255)

        cAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCatgatg                                                                                                                     <  1:89240/139‑1 (MQ=255)

           aGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCGCGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTg                                                                                                                  >  2:179689/1‑139 (MQ=255)

           aGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCat                                                                                                                         >  2:720883/1‑132 (MQ=255)

           aGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCat                                                                                                                         <  1:720883/132‑1 (MQ=255)

           aGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTg                                                                                                                  >  2:385698/1‑139 (MQ=255)

           aGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTg                                                                                                                  >  1:543715/1‑139 (MQ=255)

             cGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGa                                                                                                                <  2:117030/139‑1 (MQ=255)

                tACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCg                                                                                                             >  1:347674/1‑139 (MQ=255)

                  cTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCgtg                                                                                                           <  2:52946/139‑1 (MQ=255)

                   tGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCgtgt                                                                                                          >  2:637101/1‑139 (MQ=255)

                          acTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                          >  2:44539/1‑116 (MQ=255)

                          acTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCa                                                                                                                          <  1:44539/116‑1 (MQ=255)

                          acTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGa                                                                                                                <  2:118818/126‑1 (MQ=255)

                          acTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGa                                                                                                                >  1:118818/1‑126 (MQ=255)

                            tgctgctgGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGCCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATa                                                                                                 >  2:63037/1‑139 (MQ=255)

                                      aGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCg                                                                                       >  1:243179/1‑139 (MQ=255)

                                      aGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCg                                                                                       >  1:690265/1‑139 (MQ=255)

                                          tATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATCCTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTg                                                                                   <  2:543715/139‑1 (MQ=255)

                                                     aTACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTAcc                                                                        <  1:6557/139‑1 (MQ=255)

                                                      tACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTAccc                                                                       >  2:420557/1‑139 (MQ=255)

                                                         ccGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTc                                                                    <  1:750010/139‑1 (MQ=255)

                                                         ccGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTc                                                                    <  2:9135/139‑1 (MQ=255)

                                                          cGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCt                                                                   <  1:637101/139‑1 (MQ=255)

                                                           gAACCCGCGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTg                                                                  <  1:179689/139‑1 (MQ=255)

                                                            aaCCCACGTCTGCTGGCGAAAGATATCAATCAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTgg                                                                 <  1:472344/139‑1 (MQ=255)

                                                            aaCCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTgg                                                                 >  2:117627/1‑139 (MQ=255)

                                                            aaCCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTgg                                                                 >  1:261701/1‑139 (MQ=255)

                                                                     ctgctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTAc                                                        <  1:213121/139‑1 (MQ=255)

                                                                       gctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGaa                                                                                 >  2:360037/1‑112 (MQ=255)

                                                                       gctgGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGaa                                                                                 <  1:360037/112‑1 (MQ=255)

                                                                           gCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCgct                                                  <  2:364010/139‑1 (MQ=255)

                                                                           gCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCgct                                                  >  1:394708/1‑139 (MQ=255)

                                                                             gAAAGATATCAAACATCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCgctgc                                                <  1:63037/139‑1 (MQ=255)

                                                                             gAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCgctgc                                                <  1:478273/139‑1 (MQ=255)

                                                                             gAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCgctgc                                                <  1:331732/139‑1 (MQ=255)

                                                                               aaGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCtt                                              <  2:243179/139‑1 (MQ=255)

                                                                                             cGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAgtgt                                >  2:33472/1‑139 (MQ=255)

                                                                                                  gCACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGaa                           <  1:117627/139‑1 (MQ=255)

                                                                                                   cACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTc                                                                    <  1:210765/97‑1 (MQ=255)

                                                                                                   cACGACGGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTc                                                                    >  2:210765/1‑97 (MQ=255)

                                                                                                       acgGCGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCt                      >  1:224875/1‑139 (MQ=255)

                                                                                                           cGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCgc                                                                     <  2:548702/88‑2 (MQ=255)

                                                                                                           cGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCgc                                                                     >  1:548702/1‑87 (MQ=255)

                                                                                                           cGAGATTGTATCGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGc                  >  2:676105/1‑139 (MQ=255)

                                                                                                                      cGTTTGGTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGCGCGAAAAGCTCAGCCGTGAACgcgc       <  2:394708/139‑1 (MQ=255)

                                                                                                                           ggTCTCGATCTATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCg  <  1:420557/139‑1 (MQ=255)

TCCCCATACAAAGCGGTACTGAAAACACTGCTGCTGGAAGCCTATTCCTGGGAATACCCGAACCCACGTCTGCTGGCGAAAGATATCAAACAGCGTTTGCACGACGGCGAGATTGTATCGTTTGGTCTCGATCCATACTGCATGATGCTGGAGCGTGTTACTGAATACCTGACGGCGATTGAAGATTTTACCCGTCTGGATTTAGTACGTCGCTGCTTCTATTTAAAAGTGTGCGAAAAGCTCAGCCGTGAACGCGCCTGCG  >  NC_000913/3991921‑3992182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A