Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,218,365	C→T	E238K (GAG→AAG)	aer ←	fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,218,365	0	C	T	100.0%	137.7 / NA	43	E238K (GAG→AAG)	aer	fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/25); total (18/25)

TCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACA  >  NC_000913/3218229‑3218500

tcgttcAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTAt                                                                                                                                       >  2:325635/1‑139 (MQ=255)

       gttcaTCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCg                                                                                                                                >  2:534226/1‑139 (MQ=255)

           aTCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTc                                                                                                                            <  1:173019/139‑1 (MQ=255)

                                       ccATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCgg                                                                                                <  2:347956/139‑1 (MQ=255)

                                         aTTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTc                                                                                              <  1:683097/139‑1 (MQ=255)

                                          tttCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCg                                                                                             <  2:223559/139‑1 (MQ=255)

                                                 cacTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCtt                                                                                      <  1:63710/139‑1 (MQ=255)

                                                  acTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTc                                                                                     <  1:154465/139‑1 (MQ=255)

                                                      gACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTg                                                                                 >  2:151699/1‑139 (MQ=255)

                                                              gCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATgg                                                                         <  1:270265/139‑1 (MQ=255)

                                                                 tGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCGGTGCCTGATGGGCa                                                                      <  2:234717/139‑1 (MQ=255)

                                                                  gagaCATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCa                                                                      >  1:127101/1‑138 (MQ=255)

                                                                  gagaCATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCa                                                                      <  2:127101/138‑1 (MQ=255)

                                                                        tCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGc                                                                       <  2:169903/131‑1 (MQ=255)

                                                                        tCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGc                                                                       >  1:169903/1‑131 (MQ=255)

                                                                             aaTTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGTCCCAGCTTATCGCTGCGATTCAGATGCt                                                                                                                     <  1:477523/80‑1 (MQ=255)

                                                                             aaTTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCt                                                                                                                     >  2:477523/1‑80 (MQ=255)

                                                                                 aGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCtt                                                                                      >  1:447097/1‑107 (MQ=255)

                                                                                 aGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCtt                                                                                      <  2:447097/107‑1 (MQ=255)

                                                                                  gCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCt                                                                                                                     <  2:739468/75‑1 (MQ=255)

                                                                                  gCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCt                                                                                                                     >  1:739468/1‑75 (MQ=255)

                                                                                   ccAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGgcgc                                                    >  1:727051/1‑139 (MQ=255)

                                                                                   ccAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGgcgc                                                    >  2:178528/1‑139 (MQ=255)

                                                                                   ccAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGgcgc                                                    >  2:25712/1‑139 (MQ=255)

                                                                                   ccAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGgcgc                                                    >  1:262509/1‑139 (MQ=255)

                                                                                      aCGTCACATCAGGCCAAGTTTCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGcaca                                                 <  2:565772/139‑1 (MQ=255)

                                                                                      aCGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAg                                                                                                                          >  2:516926/1‑66 (MQ=255)

                                                                                      aCGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAg                                                                                                                          <  1:516926/66‑1 (MQ=255)

                                                                                       cGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACaa                                                >  2:626190/1‑139 (MQ=255)

                                                                                       cGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCACACaa                                                >  1:729201/1‑139 (MQ=255)

                                                                                              tCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCa                                                                      >  1:128606/1‑110 (MQ=255)

                                                                                              tCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCa                                                                      <  2:128606/110‑1 (MQ=255)

                                                                                                  gCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCa                                     >  1:661025/1‑139 (MQ=255)

                                                                                                            ccTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCa                                                                      <  1:671112/96‑1 (MQ=255)

                                                                                                            ccTACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCa                                                                      >  2:671112/1‑96 (MQ=255)

                                                                                                              tACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCtt                         <  1:435677/139‑1 (MQ=255)

                                                                                                              tACCGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCtt                         <  1:654006/139‑1 (MQ=255)

                                                                                                                 cGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGcc                      <  1:325635/139‑1 (MQ=255)

                                                                                                                 cGCACGTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGcc                      <  1:25712/139‑1 (MQ=255)

                                                                                                                    aCGTAATGTCAGCCCCAGCTTATCGCTGCGAGTCAGATGCTCAACACTATTACGTTCTCTGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCcaac                   <  2:423757/139‑1 (MQ=255)

                                                                                                                      gTAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCcaacaa                 <  1:265840/139‑1 (MQ=255)

                                                                                                                       tAATGTCAGCCCCAGCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAAt                <  2:727051/139‑1 (MQ=255)

                                                                                                                                     gCTTATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGcaca  <  2:661025/139‑1 (MQ=255)

TCGTTCAGTTCATCGGTGCCTTTCGCCAGCGTCTCACTGCCATTTCTGACACTGGACACCTGGCTTGAGACATCGTTAATTAGCCAACGGCACATCAGGCCAAGTTGCCCTACCGCACGTAATGTCAGCCCCAGCTCATCGCTGCGATTCAGATGCTCAACACTATTACGTTCTCCGGTCGCCACCTTCAGTGCCTGATGGGCAACATTTTCTATCGGGCGCACAATCTGCCATTCAAAACAGGCGCTTGCCAACAATACCACTAACGCACA  >  NC_000913/3218229‑3218500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A