Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,933,819	C→T	F14F (TTC→TTT)	rbsA →	D‑ribose ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,933,819	0	C	T	100.0%	92.3 / NA	30	F14F (TTC→TTT)	rbsA	D‑ribose ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/12); total (18/12)

AACCGCAGAAAGTCAGGCGGTAATTCGCAGCGGAGAATGTTCTCCGTATGCGAATATCATTCTCTGTGCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTCCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGTGCTTACTGGCATCTATACTCGCGATGCCG  >  NC_000913/3933686‑3933952

aaCCGCAGAAAGTCAGGCGGTAATTCGCAGCGGAGAATGTTCTCCGTATGCGAATATCATTCTCTGTGCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCggg                                                                                                                                  >  2:270739/1‑139 (MQ=255)

      aGAAAGTCAGGCGGTAATTCGCAGCGGAGAATGTTCTCCGTATGCGAATATCATTCTCTGTGCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTaaa                                                                                                                            >  1:33165/1‑139 (MQ=255)

                                             gTATGCGAATATCATTCTCTGTGCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCcg                                                                                     >  2:73284/1‑139 (MQ=255)

                                                 gCGAATATCATTCTCTGTGCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGtt                                                                                                       <  2:459303/117‑1 (MQ=255)

                                                 gCGAATATCATTCTCTGTGCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGtt                                                                                                       >  1:459303/1‑117 (MQ=255)

                                                      tatCATTCTCTGTGCTGCCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATGAAGCCTTTCCGGGCGTAAAAGCCCTCCCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGgc                                                                            >  2:441003/1‑139 (MQ=255)

                                                           ttCTCTGTGCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCtgg                                                                       >  1:126804/1‑139 (MQ=255)

                                                                  tgCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCa                                                                                                            >  1:326590/1‑95 (MQ=255)

                                                                  tgCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCa                                                                                                            <  2:326590/95‑1 (MQ=255)

                                                                  tgCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGa                                                                >  1:314821/1‑139 (MQ=255)

                                                                       gCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACg                                                           >  1:416245/1‑139 (MQ=255)

                                                                       gCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACg                                                           >  1:45391/1‑139 (MQ=255)

                                                                          tGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGgcg                                                        <  1:605268/139‑1 (MQ=255)

                                                                            aCGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGcgcg                                                      >  1:102489/1‑139 (MQ=255)

                                                                            aCGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGcgcg                                                      >  1:153143/1‑139 (MQ=255)

                                                                                      gCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATcca                                            >  2:13207/1‑139 (MQ=255)

                                                                                         gTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATccacca                                         >  1:646223/1‑139 (MQ=255)

                                                                                         gTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATccacca                                         <  2:700210/139‑1 (MQ=255)

                                                                                                 aGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGaaa                                 <  1:73284/139‑1 (MQ=255)

                                                                                                     ttACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGTGc                             >  1:478853/1‑139 (MQ=255)

                                                                                                          tCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGTGCTTACt                        <  2:126804/139‑1 (MQ=255)

                                                                                                           cAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGgcg                                                        >  2:762837/1‑106 (MQ=255)

                                                                                                           cAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGgcg                                                        <  1:762837/106‑1 (MQ=255)

                                                                                                           cAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGTGCTTACTg                       <  2:345738/139‑1 (MQ=255)

                                                                                                           cAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGTGCTTACTg                       <  2:344646/139‑1 (MQ=255)

                                                                                                             gCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGt                               <  1:84256/129‑1 (MQ=255)

                                                                                                             gCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGt                               >  2:84256/1‑129 (MQ=255)

                                                                                                                    ggCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGTGCTTACTGGCATCtata              <  1:244390/139‑1 (MQ=255)

                                                                                                                            tAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGTGCTTACTGGCATCTATACTCGCGAt      <  1:454228/139‑1 (MQ=255)

                                                                                                                                gCCTTTCCGGGGGTAAAAGCCCTCACGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGATGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGTGCTTACTGGCATCTATACTCGCGATGCCg  >  2:265758/1‑139 (MQ=255)

AACCGCAGAAAGTCAGGCGGTAATTCGCAGCGGAGAATGTTCTCCGTATGCGAATATCATTCTCTGTGCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTCCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGTGCTTACTGGCATCTATACTCGCGATGCCG  >  NC_000913/3933686‑3933952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A