Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,740,658 |
C→A |
I318I (ATC→ATA) |
avtA → |
valine‑‑pyruvate aminotransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,740,658 | 0 | C | A | 100.0%
| 96.9
/ NA
| 28 | I318I (ATC→ATA) | avtA | valine‑‑pyruvate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (11/17); total (11/17) |
TTAAGCGTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTAT > NC_000913/3740582‑3740734
|
ttAAGCGTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCca > 2:669578/1‑81 (MQ=255)
aGCGTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCcatc < 2:669579/80‑1 (MQ=255)
gCGTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCcatcat < 1:669580/81‑1 (MQ=255)
cGTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCAtt > 2:669616/1‑81 (MQ=255)
gTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTc > 1:669627/1‑81 (MQ=255)
gTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTc < 2:669581/81‑1 (MQ=255)
tAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTcg < 2:669582/81‑1 (MQ=255)
aaCGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTcgc > 1:669618/1‑81 (MQ=255)
aCGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTcgcc > 1:669610/1‑81 (MQ=255)
ctgcGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTAttt < 2:669583/80‑1 (MQ=255)
aaCAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTg < 2:669584/81‑1 (MQ=255)
aCAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGc > 1:669624/1‑81 (MQ=255)
gTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTg < 2:669585/81‑1 (MQ=255)
atcaAACCGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGAt < 1:669586/80‑1 (MQ=255)
ccGTTTTACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATaaa < 2:669587/81‑1 (MQ=255)
ttACTACCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCgg < 2:669588/80‑1 (MQ=255)
ctacCAGCGTGTTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAgg < 2:669589/81‑1 (MQ=255)
gtgtTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCAttt < 1:669591/81‑1 (MQ=255)
gtgtTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCAttt < 1:669590/81‑1 (MQ=255)
gtgtTCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCAttt > 2:669630/1‑81 (MQ=255)
ttCAGGAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTAATTCATAAACCGGAAGGAGCCATTTTcc < 2:669592/81‑1 (MQ=255)
gAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTgg > 1:669635/1‑81 (MQ=255)
gAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTgg > 1:669621/1‑81 (MQ=255)
gAAACTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTgg < 2:669593/81‑1 (MQ=255)
aCTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTa > 1:669626/1‑81 (MQ=255)
aCTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTa > 1:669625/1‑81 (MQ=255)
aCTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTa < 2:669594/81‑1 (MQ=255)
cTATAGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTAt < 2:669595/81‑1 (MQ=255)
|
TTAAGCGTAACGATCTGCTGCGCCTGTCTGAAACAGTCATCAAACCGTTTTACTACCAGCGTGTTCAGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTAT > NC_000913/3740582‑3740734
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
