Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,775,339 |
C→T |
R306C (CGT→TGT) |
mtlD → |
mannitol‑1‑phosphate 5‑dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,775,339 | 0 | C | T | 100.0%
| 124.0
/ NA
| 36 | R306C (CGT→TGT) | mtlD | mannitol‑1‑phosphate 5‑dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/20); total (16/20) |
CGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTG > NC_000913/3775261‑3775414
|
cGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGt > 1:676738/1‑81 (MQ=255)
cGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGt > 1:676730/1‑81 (MQ=255)
agaACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATc < 1:676675/80‑1 (MQ=255)
gaACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATc > 2:676745/1‑79 (MQ=255)
gaACCCGTATATGAGAGATGATGTAGAGCGTGTAGGCCGTATGCGAATGAGTAAACTGAGTGCTGGCGAGTGTCTGagc > 2:871113/1‑77 (MQ=255)
cccGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGcc < 2:676676/81‑1 (MQ=255)
ccGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCa < 1:676677/81‑1 (MQ=255)
ccGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCa > 1:676726/1‑81 (MQ=255)
cGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCAc < 1:676678/81‑1 (MQ=255)
gTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCTGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACt < 1:676679/81‑1 (MQ=255)
gTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACt > 2:676742/1‑81 (MQ=255)
cTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTc > 2:676680/1‑81 (MQ=255)
aaaGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCt < 2:676681/77‑1 (MQ=255)
aaaGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTc < 1:676682/78‑1 (MQ=255)
tgTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGa < 1:676683/81‑1 (MQ=255)
tgTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGa < 2:676684/81‑1 (MQ=255)
gagCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAata < 1:676685/80‑1 (MQ=255)
gagCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAata < 1:676686/80‑1 (MQ=255)
cgcgTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGt > 1:676746/1‑81 (MQ=255)
tAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGc < 1:676687/81‑1 (MQ=255)
ggCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCca > 1:676743/1‑81 (MQ=255)
gCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCcac < 1:676688/81‑1 (MQ=255)
cGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACAt < 1:676689/81‑1 (MQ=255)
cGTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACAt < 2:721812/81‑1 (MQ=255)
gTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACAt > 2:676735/1‑80 (MQ=255)
gTCAGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACAt > 2:676739/1‑80 (MQ=255)
aGCCACTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATaaaa < 2:676690/81‑1 (MQ=255)
aCTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCt < 2:676691/81‑1 (MQ=255)
aCTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCt > 1:676740/1‑81 (MQ=255)
cTGCGTAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTg < 2:676692/81‑1 (MQ=255)
tAAACTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTg > 1:676741/1‑80 (MQ=255)
aaCTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGaa < 1:676693/80‑1 (MQ=255)
aCTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAgg > 1:676747/1‑81 (MQ=255)
cTGAGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAgg > 2:676748/1‑80 (MQ=255)
aGTGCTGGCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTa < 2:676694/79‑1 (MQ=255)
gCGACTGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTg > 2:676755/1‑81 (MQ=255)
|
CGTTTTGAGAACCCGTATCTGAAAGATGATGTAGAGCGCGTAGGCCGTCAGCCACTGCGTAAACTGAGTGCTGGCGACCGTCTGATCAAGCCACTGCTCGGTACGCTGGAATATGGTCTGCCACATAAAAACCTGATTGAAGGTATTGCCGCTG > NC_000913/3775261‑3775414
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
