Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,834,019 |
C→T |
V173V (GTG→GTA) |
yicI ← |
alpha‑D‑xyloside xylohydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,834,019 | 0 | C | T | 100.0%
| 101.7
/ NA
| 30 | V173V (GTG→GTA) | yicI | alpha‑D‑xyloside xylohydrolase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/16); total (14/16) |
ACGGGATATTTTTATACGCCTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGCACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCTCAAACATATAAT > NC_000913/3833943‑3834095
|
aCGGGATATTTTTATACGCCTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTAc > 2:688076/1‑79 (MQ=255)
tataCGCCTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAgc > 2:688095/1‑81 (MQ=255)
ataCGCCTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAgc < 1:688044/80‑1 (MQ=255)
cTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTcc < 2:688045/81‑1 (MQ=255)
ttCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTccc < 1:688046/79‑1 (MQ=255)
cAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGAc > 1:688091/1‑81 (MQ=255)
cAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGAc > 1:688088/1‑81 (MQ=255)
aGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGAcc < 2:688047/81‑1 (MQ=255)
aGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGAc < 2:688048/80‑1 (MQ=255)
cTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTa > 1:688100/1‑80 (MQ=255)
tgtgCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAAc > 1:688107/1‑81 (MQ=255)
tgCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTg > 2:688079/1‑81 (MQ=255)
gccgTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTc > 1:688068/1‑81 (MQ=255)
gTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgc > 2:688094/1‑80 (MQ=255)
cccGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAATGCGCTCTCCCAGACCGTAAACTGTTTcgccta > 2:688103/1‑79 (MQ=255)
cccGGTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgtcaa > 2:688102/1‑81 (MQ=255)
ggTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgccgacgc < 2:688049/81‑1 (MQ=255)
ggTTCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgccaacgc < 1:688050/81‑1 (MQ=255)
ttCCAGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTcgccaacgcca < 2:688051/81‑1 (MQ=255)
aGGTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGAt < 2:688052/81‑1 (MQ=255)
ggTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATc < 1:688054/81‑1 (MQ=255)
ggTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATc < 1:688053/81‑1 (MQ=255)
gTCTCTACCGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATc > 2:688099/1‑80 (MQ=255)
ccGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGc < 2:688055/80‑1 (MQ=255)
ccGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCt < 1:688056/81‑1 (MQ=255)
cGTCTGGCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCt > 2:688093/1‑80 (MQ=255)
gCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCTCAAACat < 1:688058/81‑1 (MQ=255)
gCCATTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCTCAAACa < 1:688057/80‑1 (MQ=255)
aTTGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCTCAAACAtata < 1:688059/81‑1 (MQ=255)
tGCGTACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCTCAAACATATAAt > 2:688096/1‑81 (MQ=255)
|
ACGGGATATTTTTATACGCCTGTTCAGTACTTGTGCCGCCGTCCCGGTTCCAGGTCTCTACCGTCTGGCCATTGCGCACCAGGGCAGTAAAGCGCTCTCCCAGACCGTAAACTGTTTCGCCAACGCCAAGATCAAGCCGCTCAAACATATAAT > NC_000913/3833943‑3834095
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
