Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,924,204 |
C→T |
R477H (CGT→CAT) |
mnmG ← |
5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,924,204 | 0 | C | T | 100.0%
| 116.5
/ NA
| 33 | R477H (CGT→CAT) | mnmG | 5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/17); total (16/17) |
CGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCA > NC_000913/3924128‑3924275
|
cggcgCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAgacgctgatgct > 1:707116/1‑80 (MQ=255)
cggcgCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAgacgctgatgct > 1:707107/1‑80 (MQ=255)
cggcgCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGatgctc < 2:707050/81‑1 (MQ=255)
cggcgCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGatgctc < 2:707049/81‑1 (MQ=255)
gTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCg < 2:707051/81‑1 (MQ=255)
cAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGa > 2:707125/1‑80 (MQ=255)
aGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGat < 2:707052/80‑1 (MQ=255)
ggTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGatat < 1:707053/81‑1 (MQ=255)
cATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCaa < 1:707054/81‑1 (MQ=255)
aTTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAg < 1:707055/81‑1 (MQ=255)
tCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGtt > 1:707102/1‑81 (MQ=255)
cGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTtctc < 1:707056/79‑1 (MQ=255)
ggCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGtt < 2:707057/81‑1 (MQ=255)
gCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGtt > 1:707113/1‑80 (MQ=255)
gCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTa < 2:707058/81‑1 (MQ=255)
cTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTa > 1:707112/1‑80 (MQ=255)
gCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAg > 2:707108/1‑81 (MQ=255)
aGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAgcg > 2:707115/1‑81 (MQ=255)
cTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAgcgcg < 1:707059/81‑1 (MQ=255)
gccgACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCaa > 1:707120/1‑81 (MQ=255)
gACGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGt > 2:707119/1‑81 (MQ=255)
cGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTc < 2:707060/81‑1 (MQ=255)
cGGGGTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTc < 2:707061/81‑1 (MQ=255)
ggggTTACCCAGGTCGATTTCAGACGCTGGTGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCg > 1:707111/1‑81 (MQ=255)
ggggTTACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCg > 1:707127/1‑81 (MQ=255)
ttACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCAt < 1:707063/81‑1 (MQ=255)
ttACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCAt < 1:707062/81‑1 (MQ=255)
ttACCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCAt < 2:707064/81‑1 (MQ=255)
aCCCAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATc < 1:707065/80‑1 (MQ=255)
cccAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATcca > 1:707123/1‑81 (MQ=255)
ccAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATccac > 2:707118/1‑81 (MQ=255)
cAGGTCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATccac > 2:707114/1‑80 (MQ=255)
tCGATTTCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGc > 2:707122/1‑81 (MQ=255)
tttCAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAg > 1:707121/1‑81 (MQ=255)
cAGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGtt < 2:707066/80‑1 (MQ=255)
aGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCa < 1:707067/81‑1 (MQ=255)
aGACGCTGATGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCa > 1:707117/1‑81 (MQ=255)
|
CGGCGCAGTCAGGTGAGCATTCACTTCGGCTGCAGCTTCCGCCGACGGGGTTACCCAGGTCGATTTCAGACGCTGACGCTCACGCTCGATATTCTCAAGTTTCTCGTTAAAGCGCGCCCAACGTTCGTCATCCACCAGGCCCAGTTCA > NC_000913/3924128‑3924275
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
