Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,940,461 |
G→A |
A51V (GCC→GTC) |
hsrA ← |
putative transporter HsrA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,940,461 | 0 | G | A | 100.0%
| 140.7
/ NA
| 41 | A51V (GCC→GTC) | hsrA | putative transporter HsrA |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/21); total (20/21) |
CGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTGGCATCA > NC_000913/3940383‑3940538
|
cGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACt < 1:710587/81‑1 (MQ=255)
gACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACt > 1:710643/1‑80 (MQ=255)
aCGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGa > 2:710651/1‑81 (MQ=255)
cgcgTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGAt < 2:710588/81‑1 (MQ=255)
gTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGc < 1:710589/81‑1 (MQ=255)
gAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATcgc < 2:710590/81‑1 (MQ=255)
aaGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATcgcg < 2:710591/81‑1 (MQ=255)
gATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGagag > 1:710633/1‑81 (MQ=255)
aTCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAgagg < 1:710592/81‑1 (MQ=255)
ggCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAgaggag > 2:710634/1‑80 (MQ=255)
ggCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAgaggag > 1:710638/1‑80 (MQ=255)
cATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGAtt > 2:710637/1‑80 (MQ=255)
cGCTTACCGGAATAAGCATCGCCACCGTCAGCGTTTAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGc < 2:710593/81‑1 (MQ=255)
gCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGc > 2:710647/1‑80 (MQ=255)
gCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCt < 2:710594/81‑1 (MQ=255)
tACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTAt > 1:710595/1‑80 (MQ=255)
aCCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTAt < 2:710596/79‑1 (MQ=255)
gAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGa < 1:710597/81‑1 (MQ=255)
aaGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGc > 2:710644/1‑81 (MQ=255)
aGCATCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCg < 2:710598/81‑1 (MQ=255)
aTCGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGt < 1:710599/81‑1 (MQ=255)
cGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGt > 1:710654/1‑79 (MQ=255)
cGCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGt > 2:710639/1‑79 (MQ=255)
gCCACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTaa < 2:710600/80‑1 (MQ=255)
cACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGc > 1:710671/1‑81 (MQ=255)
cACCGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGc > 2:710648/1‑81 (MQ=255)
cGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCgg > 2:710646/1‑80 (MQ=255)
cGTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGt < 1:710601/81‑1 (MQ=255)
gTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTa > 2:710649/1‑81 (MQ=255)
gTCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTa > 2:710658/1‑81 (MQ=255)
tCAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTAt > 1:710656/1‑81 (MQ=255)
cAGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTAt > 2:710660/1‑80 (MQ=255)
aGCGTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTAtt < 1:710602/80‑1 (MQ=255)
gTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAg > 2:710657/1‑80 (MQ=255)
gTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGa < 1:710603/81‑1 (MQ=255)
gTATAACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGa < 1:710604/81‑1 (MQ=255)
taACTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAAt < 2:710605/80‑1 (MQ=255)
cTGATGATGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTg < 1:710606/81‑1 (MQ=255)
tgatgatgaCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTgg > 2:710673/1‑81 (MQ=255)
tgatgatgaCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTgg > 2:710682/1‑81 (MQ=255)
gatgatGACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTgg > 2:710655/1‑80 (MQ=255)
atgatgACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTGGc < 2:710607/80‑1 (MQ=255)
atgACTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTGGCATCa < 1:710608/81‑1 (MQ=255)
|
CGACGCGTACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTGGCATCA > NC_000913/3940383‑3940538
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
