Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,985,601 |
G→A |
S136F (TCT→TTT) |
aslA ← |
arylsulfatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,985,601 | 0 | G | A | 100.0%
| 97.1
/ NA
| 29 | S136F (TCT→TTT) | aslA | arylsulfatase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/16); total (13/16) |
CCCGTACATTGGCGGCATCAGAATGCCGTGGTGGATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTGTAGGGTTACC > NC_000913/3985525‑3985677
|
cccGTACATTGGCGGCATCAGGATGCCGTGGTGGATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGc < 2:720526/80‑1 (MQ=255)
ccGTACATTGGCGGCATCAGAATGCCGTGGTGGATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCtt < 2:720527/81‑1 (MQ=255)
cGTACATTGGCGGCATCAGAATGCCGTGGTGGATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTg > 1:720564/1‑81 (MQ=255)
gTACATTGGCGGCATCAGAATGCCGTGGTGGATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTgg < 2:720528/81‑1 (MQ=255)
gTACATTGGCGGCATCAGAATGCCGTGGTGGATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTgg > 1:720571/1‑81 (MQ=255)
tACATTGGCGGCATCAGAATGCCGTGGTGGATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTGGt > 1:720584/1‑81 (MQ=255)
ttGGCGGCATCAGAATGCCGTGGTGGATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTGGTTgag < 2:720529/81‑1 (MQ=255)
ggcggcATCAGAATGCCGTGGTGGATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTGGTTGAGaa > 2:720579/1‑81 (MQ=255)
gcATCAGAATGCCGTGGTGGATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTGGTTGAGAATACg < 2:720530/81‑1 (MQ=255)
aTCAGAATGCCGTGGTGGATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTGGTTGAGAATACGcc < 2:720531/81‑1 (MQ=255)
aGAATGCCGTGGTGGATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTGGTTGAGAATACGCCg > 1:720563/1‑79 (MQ=255)
aGAATGCCGTGGTGGATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTGGTTGAGAATACGCCGaa < 1:720532/81‑1 (MQ=255)
aaTGCCGTGGTGGATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTGGTTGAGAATACGCCGAAGt < 2:720533/81‑1 (MQ=255)
aaTGCCGTGGTGGATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTGGTTGAGAATACGCCGAAGt < 2:720534/81‑1 (MQ=255)
atggaGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTGGCTGAGAATACGCCGAAGTTAAAATCAGcccc > 1:720567/1‑81 (MQ=255)
ggaGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCctg > 1:720575/1‑81 (MQ=255)
tATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCctggctg < 2:720535/81‑1 (MQ=255)
ttGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCctggctgg > 1:720569/1‑80 (MQ=255)
ttGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCtggctggc < 1:720536/81‑1 (MQ=255)
gTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCaa > 1:720566/1‑81 (MQ=255)
tCCGGTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAAc < 1:720537/81‑1 (MQ=255)
ggTGAGAATGGTGGCGCGGGTTGGGGAAAAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGc > 2:720583/1‑81 (MQ=255)
agaATGGTGGCGCGGGTTGGGGAAAAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTc < 2:720538/80‑1 (MQ=255)
aaTGGTGGCGCGGGTTGGGGAAAAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGat < 2:720539/81‑1 (MQ=255)
ggtggCGCGGGTTGGGGAAAAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATc > 2:720574/1‑81 (MQ=255)
tggCGCGGGTTGGGGAAAAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTg < 1:720540/81‑1 (MQ=255)
gcgcGGGTTGGGGAAAAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGgt < 1:720541/81‑1 (MQ=255)
gggAAAAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTGTAGGGTTAc > 1:720580/1‑81 (MQ=255)
gAAAAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTGTAGGGTTAcc > 1:720560/1‑80 (MQ=255)
|
CCCGTACATTGGCGGCATCAGAATGCCGTGGTGGATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTGTAGGGTTACC > NC_000913/3985525‑3985677
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
