Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A1 F1 I2 R1 | 524 | 31.4 | 1748232 | 97.8% | 1709770 | 80.6 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,020,179 | C→T | R186C (CGT→TGT) noncoding (202/252 nt) |
ubiJ → esrE → |
ubiquinone biosynthesis accessory factor UbiJ small RNA EsrE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,020,179 | 0 | C | T | 100.0% | 110.4 / NA | 32 | R186C (CGT→TGT) noncoding (202/252 nt) | ubiJ esrE | ubiquinone biosynthesis accessory factor UbiJ small RNA EsrE |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (12/20); total (12/20) | |||||||||||
ACTGAAGAGTGGCGTATGGCACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGCGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTGAAGTACGGCGCCTATAT > NC_000913/4020107‑4020255 | aCTGAAGAGTGGCGTATGGCACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGtt > 1:728211/1‑81 (MQ=255) aaGAGTGGCGTATGGCACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGAt < 1:728172/80‑1 (MQ=255) gagTGGCGTATGGCACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGcc < 1:728173/81‑1 (MQ=255) gTGGCGTATGGCACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCt < 2:728174/81‑1 (MQ=255) tGGCACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAAc > 1:728226/1‑81 (MQ=255) gCACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACgg > 2:728209/1‑81 (MQ=255) aCCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCt > 2:728227/1‑81 (MQ=255) cccGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTg < 2:728175/81‑1 (MQ=255) ccGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTgg < 1:728176/81‑1 (MQ=255) ccGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTATTGATGCCCTGACCAAACGGCTg > 1:728221/1‑80 (MQ=255) ggTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGaa < 2:728177/81‑1 (MQ=255) tGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACTAAACGGTTGGAAAAACTg > 1:728225/1‑79 (MQ=255) aGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGc < 1:728178/81‑1 (MQ=255) gCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTaa < 1:728179/80‑1 (MQ=255) ccTGGTTTGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAAt > 2:728228/1‑81 (MQ=255) tGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGcc < 1:728181/80‑1 (MQ=255) tGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGcc < 2:728180/80‑1 (MQ=255) tGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGcc < 2:728182/80‑1 (MQ=255) tGCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGc > 2:728244/1‑79 (MQ=255) tGCGGAAGAGACGGCTGCCGTCGAGTGTGCTATTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGc > 1:728231/1‑79 (MQ=255) gCGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAg > 2:728229/1‑81 (MQ=255) cGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAgg > 2:728223/1‑81 (MQ=255) cGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAgg > 2:728222/1‑81 (MQ=255) cGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAg < 2:728183/80‑1 (MQ=255) cGGAAGAGACGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAAAGGCGCCAgg < 2:728184/81‑1 (MQ=255) agaCGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTGAAGt < 1:728185/81‑1 (MQ=255) agaCGGCTGCCGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTGAAGt < 1:728186/81‑1 (MQ=255) ccGTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTGAAGTACGGCGCCt < 2:728188/81‑1 (MQ=255) ccGTCGAGTGTGCTATTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTGAAGTACGGCGcc < 1:728187/80‑1 (MQ=255) gTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTGAAGTACGGCGCCtat < 2:728190/81‑1 (MQ=255) gTCGAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTGAAGTACGGCGCCtat < 2:728189/81‑1 (MQ=255) gAGTGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTGAAGTACGGCGCCTatat < 2:728191/80‑1 (MQ=255) | ACTGAAGAGTGGCGTATGGCACCCGGTCCGCTTGAAGTGGCCTGGTTTGCGGAAGAGACGGCTGCCGTCGAGCGTGCTGTTGATGCCCTGACCAAACGGCTGGAAAAACTGGAGGCTAAATGACGCCAGGTGAAGTACGGCGCCTATAT > NC_000913/4020107‑4020255 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |