Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,033,383 |
T→A |
L80Q (CTG→CAG) |
trkH → |
K(+) transporter TrkH |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,033,383 | 0 | T | A | 96.8%
| 103.9
/ ‑4.6
| 31 | L80Q (CTG→CAG) | trkH | K(+) transporter TrkH |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (13/17); minor base G (0/1); total (13/18) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 |
CGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTT > NC_000913/4033311‑4033453
|
cGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCg > 2:794360/1‑81 (MQ=255)
aaCCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTc < 1:794312/81‑1 (MQ=255)
aaCCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTc < 2:794313/81‑1 (MQ=255)
aaCCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTc < 1:794311/81‑1 (MQ=255)
aaaGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTgcg > 1:794361/1‑81 (MQ=255)
aaaGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTgcg > 2:794350/1‑81 (MQ=255)
aaGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTgcgc < 1:794314/81‑1 (MQ=255)
gagaAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTc > 1:794362/1‑81 (MQ=255)
aaaGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCt > 1:794351/1‑81 (MQ=255)
aaaGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCt > 2:794352/1‑81 (MQ=255)
aaaGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCt < 1:794315/81‑1 (MQ=255)
aGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCttt < 1:794316/81‑1 (MQ=255)
gAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTAtc < 2:794317/81‑1 (MQ=255)
aaaTCCCGTGGGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTtctc < 2:794318/80‑1 (MQ=255)
aaaTCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTAtcttct < 2:794319/79‑1 (MQ=255)
aaTCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCgg < 1:794320/81‑1 (MQ=255)
aaTCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCg > 1:794355/1‑80 (MQ=255)
gTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGcc > 1:794363/1‑81 (MQ=255)
gTTTCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCt > 1:794371/1‑81 (MQ=255)
tttCTGATAGTGGTGCTGTTCTGGACCGTGCGGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTc < 2:794321/81‑1 (MQ=255)
tttCTGATAGTGGTGCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCt > 2:794370/1‑80 (MQ=255)
gtggtgCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTAcc < 2:794323/81‑1 (MQ=255)
gtggtgCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTAcc < 1:794322/81‑1 (MQ=255)
gtgCTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGAt < 1:794324/81‑1 (MQ=255)
cTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTTGGAAAGCCCGAACCTCACGATTACCGATGCg > 2:794374/1‑81 (MQ=255)
cTGTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGc < 1:794325/80‑1 (MQ=255)
gTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGtt > 1:794356/1‑81 (MQ=255)
gTTCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGtt > 2:794359/1‑81 (MQ=255)
ttCTGGACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGttt < 2:794326/81‑1 (MQ=255)
gACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGtttttt < 2:794328/79‑1 (MQ=255)
gACCGTGCAGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGttttt < 2:794327/78‑1 (MQ=255)
|
CGAACCGCAAAGAGAAAGGCGAACTTAAATCCCGTGAGGGGTTTCTGATAGTGGTGCTGTTCTGGACCGTGCTGGGTAGCGTCGGTGCGCTCCCTTTTATCTTCTCGGAAAGCCCGAACCTCACGATTACCGATGCGTTTTTT > NC_000913/4033311‑4033453
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
